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Ecotoxicology

, Volume 28, Issue 3, pp 354–366 | Cite as

The novel pesticide flupyradifurone (Sivanto) affects honeybee motor abilities

  • Hannah HesselbachEmail author
  • Ricarda Scheiner
Article

Abstract

Honeybees and other pollinators are threatened by changing landscapes and pesticides resulting from intensified agriculture. In 2018 the European Union prohibited the outdoor use of three neonicotinoid insecticides due to concerns about pollinators. A new pesticide by the name of “Sivanto” was recently released by Bayer AG. Its active ingredient flupyradifurone binds to the nicotinic acetylcholine receptor (AchR) in the honeybee brain, similar to neonicotinoids. Nevertheless, flupyradifurone is assumed to be harmless for honeybees and can even be applied on flowering crops. So far, only little has been known about sublethal effects of flupyradifurone on honeybees. Intact motor functions are decisive for numerous behaviors including foraging and dancing. We therefore selected a motor assay to investigate in how far sublethal doses of this pesticide affect behavior in young summer and long-lived winter honeybees. Our results demonstrate that flupyradifurone (830 µmol/l) can evoke motor disabilities and disturb normal motor behavior after a single oral administration (1.2 µg/bee). These effects are stronger in long-lived winter bees than in young summer bees. After offering an equal amount of pesticide (1.0–1.75 µg) continuously over 24 h with food the observed effects are slighter. For comparisons we repeated our experiments with the neonicotinoid imidacloprid. Intriguingly, the alterations in behavior induced by this pesticide (4 ng/bee) were different and longer-lasting compared to flupyradifurone, even though both substances bind to nicotinic acetylcholine receptors.

Keywords

Honeybee Motor ability Acetylcholine receptor Insecticide Flupyradifurone Imidacloprid 

Notes

Acknowledgements

We thank our departmental beekeeper Dirk Ahrens for beekeeping. We thank Laura Degirmenci for help with the manuscript.

Funding

We thank the Heinrich Stockmeyer Stiftung, Bad Rothenfelde, for financial support to HH.

Author contributions

HH gained data. Both authors wrote the main manuscript text and HH prepared all of the figures. Both authors reviewed the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10646_2019_2028_MOESM1_ESM.pdf (317 kb)
Supplementary Information.
10646_2019_2028_MOESM2_ESM.mp4 (968 kb)
Supplementary Movie 1.
10646_2019_2028_MOESM3_ESM.mp4 (688 kb)
Supplementary Movie 2.

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Authors and Affiliations

  1. 1.Biocenter, Behavioral Physiology and Sociobiology, Am HublandUniversity of WürzburgWürzburgGermany
  2. 2.Faculty of Veterinary Medicine, Institute of Veterinary Anatomy, Histology and EmbryologyUniversity of LeipzigLeipzigGermany

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