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Acute toxicity assessment of N,N-diethyl-m-toluamide (DEET) on the oxygen flux of the dinoflagellate Gymnodinium instriatum

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Abstract

Despite the ubiquitous occurrence of N,N-diethyl-m-toluamide (DEET) in aquatic systems, assessments evaluating the toxicity of DEET on phytoplankton species are summed to a single study on a unicellular green alga. In particular, the toxicological effects of DEET in dinoflagellates are unknown. In this study, we employed the mixotrophic dinoflagellate Gymnodinium instriatum as a study system to evaluate acute effects of DEET on the oxygen flux of laboratory cultures. This study reports an inhibitory reaction model of DEET described by the equation y = 4.99x0.54, where y represents the percent inhibition of oxygen flux and x represents DEET concentration in mg L−1 (r2 = 0.98). Based on this model, the effective concentration of DEET needed to reduce O2 flux by 50 % (EC50) for this species was found to be at 72.9 mg L−1. The reported EC50 is more than five times lower than the EC50 reported previously for the unicellular green algae Chlorella protothecoides. This study raises the question of the potential toxicological effects of DEET in dinoflagellates, in particular those populations inhabiting systems characterized by low water circulation such as enclosed bays and lagoons.

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Acknowledgments

The authors will like to extent our gratitude to Dr. Joseph J. Torres, who kindly provided both high-resolution respirometry systems used in this study. This research was funded by the National Science Foundation (Grant Number OCE-0727883), awarded to Dr. Joseph J. Torres.

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Authors and Affiliations

  1. Center for Environmental Studies, Virginia Commonwealth University, 1000 W Cary St., Richmond, VA, 23284, USA

    Eloy Martinez

  2. Universidad de Puerto Rico en Humacao, Carretera 908 Km. 1.2, Humacao, PR, 00792, USA

    Sylvia M. Vélez & Miguel P. Sastre

  3. College of Marine Science, University of South Florida, 140 7th Ave S, Saint Petersburg, FL, 33701, USA

    Marietta Mayo

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  1. Eloy Martinez
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  2. Sylvia M. Vélez
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Correspondence to Eloy Martinez.

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Martinez, E., Vélez, S.M., Mayo, M. et al. Acute toxicity assessment of N,N-diethyl-m-toluamide (DEET) on the oxygen flux of the dinoflagellate Gymnodinium instriatum . Ecotoxicology 25, 248–252 (2016). https://doi.org/10.1007/s10646-015-1564-z

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