Surfactants decrease the toxicity of ZnO, TiO2 and Ni nanoparticles to Daphnia magna
The objective of the study was the estimation of the effect of surfactants on the toxicity of ZnO, TiO2 and Ni nanoparticles (ENPs) towards Daphnia magna. The effect of hexadecyltrimethylammonium bromide (CTAB), triton X-100 (TX100) and 4-dodecylbenzenesulfonic acid (SDBS) was tested. The Daphtoxkit F™ test (conforming to OECD Guideline 202 and ISO 6341) was applied for the toxicity testing. Both the surfactants and the ENPs were toxic to D. magna. The addition of ENPs to a solution of the surfactants caused a significant reduction of toxicity of ENPs. The range of reduction of the toxicity of the ENPs depended on the kind of the ENPs and their concentration in the solution, and also on the kind of surfactant. For nano-ZnO the greatest reduction of toxicity was caused by CTAB, while for nano-TiO2 the largest drop of toxicity was observed after the addition of TX100. In the case of nano-Ni, the effect of the surfactants depended on its concentration. Most probably the reduction of toxicity of ENPs in the presence of the surfactants was related with the formation of ENPs aggregates that inhibited the availability of ENPs for D. magna.
KeywordsNanoparticles Daphnia magna Surfactants Toxicity
In the near future engineered nanoparticles (ENPs) may become a new contaminant for the environment. This is indicated by the great increase of their production and application in various areas of life (Gottschalk et al. 2013). Both the production and the use of materials containing ENPs in their composition may, as indicated by recent research, lead to their release into environment (Gottschalk et al. 2011; Windler et al. 2012). Therefore it is extremely important to acquire detailed knowledge on the fate of ENPs in the environment, and especially on the factors that may have a significant effect on their mobility and, in particular, their toxicity towards various groups of organisms. Stability of ENPs in aqueous environments is a key factor controlling their transport and fate in aqueous environments (Sharma 2009; Lowry et al. 2012). In the aquatic environment ENPs interact among themselves and with other ENPs or larger particles. This process is determined by the properties of the ENPs as well as ENPs interactions with other compounds, both natural (e.g. natural organic matter, aquatic colloids) and anthropogenic (e.g. surfactants) (Lin et al. 2010). Aggregation reduces the overall specific surface area of ENPs and interfacial free energy and thus will limit the reactivity of ENPs (Saleh et al. 2008; Prathna et al. 2011; Lowry et al. 2012). The literature provides frequent indications of the effect of humic and fulvic acids on the aggregation of CNTs (Saleh et al. 2008). Another group that may affect ENPs solubility, mobility and dispersion are surfactants (Yang et al. 2010; Oleszczuk and Xing 2011). For example, nano-ZnO coated with the surfactant sodium dodecyl sulfate was stable in soil suspension for 14 days without changes in particle size distribution (Gimbert et al. 2007). Surfactants are often used to purify ENPs or as dispersants for application purposes (Bhushan 2010). Non-ionic and ionic surfactants are commonly used e.g. to coat nano-TiO2 to remain dispersed (i.e. stable) during the fabrication of paints and cosmetics (Tkachenko et al. 2006). In addition, in ecotoxicological studies surfactants are frequently used for the stabilization of ENPs, which may have a direct effect on toxicity. In view of the above information it is, therefore, extremely important to acquire knowledge on the effect of surfactants on the toxicity of ENPs. The few studies conducted so far have been concerned with plants and indicated an increase in the toxicity of ENPs in the presence of surfactants (Barrena et al. 2009; Stampoulis et al. 2009). Stampoulis et al. (2009) showed that sodium dodecyl sulfate confounded and, in most cases, amplified, the effects of ENPs on exposed C. pepo plants. Similarly, stabilizer coatings used to ensure the dispersibility and stability of Au, Ag, and Fe3O4 ENPs in water affected Cucumis sativus and Lactuca sativa (lettuce) seeds more than the ENPs alone (Barrena et al. 2009). Whereas, there is a lack of information on how surfactants may affect the toxicity of ENPs with relation to other organisms. Studies show that the toxicity of surfactants depends not only on their kind (cationic, anionic and non-ionic) but also on a number of other factors (e.g. their structure) (Ying 2006). Therefore, depending on what surfactant is used for the stabilization of ENPs one can expect diverse effects both on the part of the surfactants themselves, and on that of the surfactants and the ENPs.
The objective of the study presented here was the estimation of the toxicity of various ENPs—nano-ZnO, nano-TiO2 and nano-Ni in the presence of ionic (cationic—hexadecyltrimethylammonium bromide and anionic—4-dodecylbenzenesulfonic acid) and non-ionic (Triton X-100) surfactants towards Daphnia magna. It is estimated that among inorganic nanomaterials (apart from Ag) the highest production is characteristic of nano-ZnO and nano-TiO. ZnO and TiO2 NPs are widely used in the consumer products (sunscreen products, textiles, paints, coatings and antibacterial agents) which needs the detailed assessment of their potential toxicity to different organisms. The growing scale of production of NPs involves the risk of their release into the environment. Ni nanoparticles, on the other hand, are used in production catalysts, battery electrodes and diesel–fuel additives and also may released to environment. While for ZnO and TiO2 there are a lot of data on their toxicity, in the case of Ni NPs data on this subject are limited. Thus, it is important to evaluate the effect of the surfactants on both the common nanoparticles, such as ZnO and TiO2 but also less popular NPs, such as Ni NPs.
Materials and methods
Nanoparticles ZnO (nano-ZnO), TiO2 (nano-TiO2, mainly anatase form) and Ni (nano-Ni) were purchased from Sigma-Aldrich (USA). CAS numbers of used metal and metal oxide nanoparticles were: 1314-13-2 (nano-ZnO), 13463-67-7 (nano-TiO2), 7440-02-0 (nano-Ni). The ENPs (the purity was around 99.5 %) were used as powder. The primary particle size of ENPs was as follows: nano-ZnO < 100 nm; nano-TiO2 < 21 nm; nano-Ni < 100 nm. The size of ENPs was determined by transmission electron microscope (JEM-3010 TEM JEOL, Ltd., Japan). Surfactants (4-dodecylbenzenesulfonic acid—SDBS, hexadecyltrimethylammonium bromide—CTAB, triton X-100—TX-100) were purchased from Sigma-Aldrich (USA). All solutions were prepared using analytical grade reagents and HPLC grade water (POCH, Gliwice, Poland).
Samples of ENPs as well as surfactants were prepared in the ISO medium for Daphtoxkit F bioassay (5.75 mg/L KCl, 64.75 mg/L NaHCO3, 123.25 mg/L MgSO4 × 7H2O, 294.0 mg/L CaCl2 × 2H2O). In the each steps of experiment, the standard ISO medium without ENPs and surfactants was used as a control.
The first to be determined was the toxicity of solutions of the ENPs, and that of the surfactants. The toxicity of the ENPs was assayed within the range of concentrations from 0.05 to 1000 mg/L. Whereas, the toxicity of the surfactants was determined within the range of concentrations from 0.005 to 0.5 mg/L for SDBS and CTAB, and from 0.6 to 500 mg/L for TX100. The different ranges of surfactants concentration were tested because of their various toxicity towards D. magna.
For the purpose of determination of the effect of the surfactants on the toxicity of the ENPs such concentrations of the surfactants were chosen that caused immobility of the test organisms at the level of 10 %, i.e. for SDBS and CTAB-0.01 mg/L and for TX100-1 mg/L. The ENPs were added to surfactant solution, at the same range of concentrations at which their own toxicity was assayed (0.05–1000 mg/L). The solutions of ENPs and of the ENPs with surfactants were sonicated for 30–minute at temperature of 25 °C in an ultrasound bath (Polsonic, 250 W, 50 Hz) before application on the test plates.
The Daphtoxkit F™ bioassay (Microbiotest, Ghent, Belgium) was used to estimate effect of surfactants on the toxicity of ENPs to crustacean D. magna. The whole procedure was carried out according to the user’s manual (Daphtoxkit 1996). The Daphtoxkit F test is performed in accordance with test procedures of OECD Guideline 202 and ISO 6341. The each test vessel contained 20 mL of the test solution and ten neonates (less than 24 h old).After 48 h the number of dead neonates was estimated. In order to check the correct execution of the test animals, the reference test was conducted with using the reference toxicant potassium dichromate (K2Cr2O7). The quality control test was successful.
The ISO medium with ENPs alone and ENPs with surfactants was characterized using dynamic light scattering (DLS) (Zetasizer 3000, Malvern), transmission electron microscopy (TEM) (Tecnai G2 T20 X-TWIN, FEI) and scanninig electron microscopy with energy dispersive spectrometry (SEM–EDS) (Quanta™ 3D FEG, FEI with EDAX SDD Apollo detector). For these analysis, the samples were prepared in following way: NPs at concentration of 100 mg/L were suspended in: (1) the ISO medium used in the test, (2) SDBS solution (0,01 mg/L), (3) CTAB solution (0,01 mg/L) and (4) TX-100 solution (1 mg/L) and pontificated for 30 min. The size of aggregates and zeta potential were measured by DLS technique (Zetasizer 3000, Malvern). The pH and O2 of samples was measured. The SEM–EDS analyses were conducted to observe nanoparticles on/inside D. magna. The SEM–EDS measurements were made with the high vacuum with accelerating voltage mode. Using the EDS maps it is possible to see Zn, Ti and Ni particles in/on D. magna. The EDS diagrams (below SEM–EDS maps) show spectrums, which present characteristics X-rays of sample atoms induced by electron beam (30 keV). The SEM–EDS maps (with 50-fold magnification) and were obtained for whole organism just after the exposure time. The EDS spectra confirm and correspond with the contribution of elements (Zn, Ti, Ni) in the whole sample area. In this particular case the organisms were taken from the solutions of NPs at concentration of 100 mg/L suspended in: (1) the ISO medium used in the test, (2) SDBS solution (0,01 mg/L), (3) CTAB solution (0,01 mg/L) and (4) TX-100 solution (1 mg/L).
Effect concentrations (EC) were derived from full log-logistic (surfactants) or linear (ENPs) concentration effect curves. The reported EC50 values are the average of three independently replicates. The differences between toxicological data (EC50 for NPs only and NPs with surfactants) were evaluated using the Kruskal–Wallis test followed by post hoc Nemenyi test. Other differences between NPs and NPs with surfactants (particle size or zeta potential) were determined using a one-way analysis of variance (ANOVA) followed by Dunnett’s post hoc test.
Results and discussion
Effect of surfactants on D. magna
The available literature data on the toxicity of surfactants are sparse. Surfactants input into environment through the discharge of sewage effluents into surface waters and application of sewage sludge on land. Therefore, estimation of the toxicity of surfactants is very important in the prediction of environmental hazard related with their presence in the environment. Lewis (1991) observed a chronic and sub-lethal toxicity of anionic, cationic and non-ionic surfactants with relation to aquatic organisms, that appeared at concentrations higher than 0.1 mg/L. Emmanuel et al. (2005) studied the toxicity of three surfactants (CTAB, TX100 and SDS) towards D. magna and observed that the toxicity of the surfactants was as follows: cationic surfactants > anionic surfactants > non-ionic surfactants. The same tendency was observed in the present study (Fig. 1). The highest toxicity towards D. magna was characteristic of CTAB, followed by SDBS. TX-100 was characterised by the lowest toxicity (Fig. 1). The values of EC50 calculated on the basis of the results were similar to those presented by Panouillères et al. (2007) and Emmanuel et al. (2005). The values of EC50 determined by those authors for D. magna were 0.087/0.024, 41.2/29.2 and 89.3/38.1 mg/L, respectively, for CTAB, SDBS and TX100. The sole difference observed in this study was a significantly higher toxicity of SDBS.
Behaviour of ENPs in ISO medium in presence of surfactants
The toxicity of ENPs to D. magna
Influence of surfactants on ENPs toxicity to D. magna
The addition of the ENPs to solutions of all the surfactants caused a reduction of their toxicity within the whole range of concentrations applied (Fig. 6). The exception were particles of nano-ZnO, in the case of which at the highest concentration tested (1000 mg/L) no significant differences were noted between the toxicity in the solutions with and those without the surfactants (Fig. 6a). The greatest reduction of toxicity for nano-ZnO was observed after the application of CTAB. Depending on the nano-ZnO concentration, the reduction of toxicity of nano-ZnO varied from 30 (100 mg/L) to 100 % (the total reduction of toxicity in the lowest nano-ZnO concentration). The presence of both TX100 and SDBS reduced the toxicity of nano-ZnO at a similar level which varied from 15 to 87 %. The greatest reduction of toxicity (>60 %) was observed at the lowest range of concentrations (0.05–0.5 mg/L nano-ZnO) (Fig. 6a). As in the case of nano-ZnO, all of the surfactants under study also reduced the toxicity of nano-TiO2. However, the reduction of toxicity was not as significant as was the case with nano-ZnO. Also, distinct differences were noted in the reduction of toxicity among the surfactants (Fig. 6b). In the case of TiO2 the best reduction was obtained for TX100 (80–100 %/the total reduction of toxicity), followed by CTAB (55–100 %/the total reduction of toxicity) and finally SDBS (25–66 %). As opposed to nano-ZnO, greater reduction of toxicity was observed for higher concentrations of nano-TiO2. In addition, even at the highest concentration tested the toxicity of nano-TiO2 in solutions containing the surfactants was significantly lower compared to the solution without any surfactants. The toxicity of nano-Ni was also reduced under the effect of the surfactants, with no significant differences observed among the surfactants for most of the concentrations tested. The reduction of toxicity (from 0.05 to 10 mg/L nano-Ni) varied from 60 to 100 % (the total reduction of toxicity). Only at the highest concentration tested significant differences were observed among all the surfactants. The best reduction was obtained for TX100 (80 %), followed by SDBS (70 %), and the lowest for CTAB (60 %) for the highest concentration of nano-Ni.
Studies on the effect of surfactants on the toxicity of ENPs are relatively scarce (Barrena et al. 2009; Stampoulis et al. 2009) even though their effect on the behaviour of ENPs in the environment is commonly known (Tkachenko et al. 2006). Stampoulis et al. (2009), similarly to Barrena et al. (2009), observed an increase of the toxicity of ENPs in the presence of surfactants with relation to plants. The results obtained in this study are different, which is most probably related with the fact of using a different test organism.
Although a reduction of toxicity after the addition of the surfactants was observed for all ENPs, the hypothesis suggested earlier that the reduction of bioavailability or surface coating reduces the toxicity of ENPs in the presence of surfactants cannot be applied to nano-TiO2 and nano-Ni. Both for nano-TiO2 (Fig. 7b) and nano-Ni (Fig. 7c) the concentration of ENPs on the surface/inside of D. magna was distinctly higher than or similar to that in solutions containing nano-TiO2 in SDBS solution and nano-TiO2 in CTAB solution as well as nano-Ni in SDBS solution and nano-Ni in CTAB solution compared to experiment without surfactants. In spite of that, the surfactants reduced the toxic effect of the ENPs. However only in the case of nano-TiO2 clear regularity may be observed. One can clearly see that the reduction of toxicity (Fig. 6b) in the presence of surfactants depends on the concentration of Ti on the surface/inside of D. magna (Fig. 7b). No similar relation was noted in the case of nano-Ni (Fig. 7c). Analysing the results relating to nano-TiO2 and nano-Ni one should assume that the reduction of toxicity of ENPs in the presence of surfactants may have another/additional mechanism than just accumulation/surface coating of ENPs. Gaiser et al. (2011) suggested that besides particle size and solubility, interactions between particles and food materials in the test media may affect the toxicity of ENPs. However, during the test (D. magna was only feed before experiment according to the procedure), which excludes that factor as a possible one. Das et al. (2013) suggest that the toxicity of ENPs is a combination of the release of ions from particles and D. magna direct interactions with the ENPs. Reduction of toxicity undoubtedly results from interactions of ENPs with surfactant particles. Dabrunz et al. (2011) demonstrated that after a few hours most of nano-TiO2 sink to the bottom of the test beakers. The addition of surfactants to the solution increases the size of aggregates, as a result of which they become heavier and sediment faster compared to ENPs alone (Chibowski et al. 2007). ENPs settled on the substrate are not only harder to uptake by D. magna than when suspended in the solution, but also direct contact of ENPs with D. magna is limited. In addition, the binding of ENPs by surfactants may inhibit the solubility of ZnO and Ni, which—according to certain authors—determines primarily the ecotoxicity of ENPs. That last issue, however, requires additional research.
The presence of surfactants considerably reduced the toxicity of all tested ENPs. Although earlier studies showed that surfactants may increase the toxicity of ENPs towards plants, an opposite tendency was observed in this study. This indicates that in the analysis of the toxicity of ENPs it is very important to take into account various groups of organisms, because of different potential mechanisms of ENPs toxicity. Generalization of results may lead to erroneous conclusions. A positive aspect of the results obtained is that the toxicity of ENPs can be reduced as a result of their contact with surfactants, which reduces the risk to the environment. On the other hand, however, surfactants—increasing the aggregation of ENPs—reduce their mobility and that may mean a longer time of retention of those contaminants. These problems assume a growing importance in view of the every greater production of surfactants and ENPs alone. It should also be emphasised that surfactants are used for the stabilisation of ENPs in ecotoxicological studies. That may lead to incorrect estimation of the toxicity of ENPs. Underestimation of the environmental hazard may lead to serious environmental consequences, with potential effect on various groups of organisms.
The work was funded in the frame of Grant No. N523 616639 financed in 2010–2012 from the budget of Ministry of Science and Information Society Technologies (Poland).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
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