, Volume 24, Issue 7–8, pp 1540–1547 | Cite as

Cultivation-dependent analysis of the microbial diversity associated with the seagrass meadows in Xincun Bay, South China Sea

  • Yu-Feng Jiang
  • Juan Ling
  • You-Shao WangEmail author
  • Biao Chen
  • Yan-Ying Zhang
  • Jun-De DongEmail author


Microbial communities have largely existed in the seagrass meadows. A total of 496 strains of the bacteria in the seagrass meadows, which belonged to 50 genera, were obtained by the plate cultivation method from three sites of Xincun Bay, South China Sea. The results showed that Bacillales and Vibrionales accounted for the highest proportions of organisms in all communities. The diversity of the bacteria in the sediment was higher than that associated with seagrass. Thalassia hemperichii possessed the highest abundance of bacteria, followed by Enhalus acoroides and Cymodocea rotundata. Robust seasonal dynamics in microbial community composition were also observed. It was found that microbial activities were closely tied to the growth stage of the seagrass. The microbial distribution was the lowest in site 3. The abundance of the bacteria was linked to the interactions between bacteria and plants, the condition of plant and even the coastal water quality and the nutrition level in the sediment.


South China Sea Xincun Bay The seagrass meadows Bacteria 16S rRNA gene Cultivation 



This research was supported by the National Natural Science Foundation of China (No.41276113, No.41276114, No.41006069, No.41406191 and No. 41430966), the National High Technology Research and Development Program of China (No.2012AA092104, No.2013AA092901 and No.2013AA092902), the Strategic Priority Research Program of the Chinese Academy of Sciences (No.XDA11020202), the Science and technology cooperation projects of Sanya (No.2013YD74), and the Sanya Station Database and the Information System of CERN, the Knowledge Innovation Program of the Chinese Academy of Sciences (No.SQ201218), the Open Fund of Key Laboratory for Ecological Environment in Coastal Areas, State Oceanic Administration (No.201304).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


  1. An QL (2014) Complete genome sequence of Kosakonia sacchari type strain SP1T. Stand Genomic Sci 9:1311–1318CrossRefGoogle Scholar
  2. Andrews JH, Harris RF (2000) The ecology and biogeography of microorganisms on plant surfaces. Annu Rev Phytopathol 38:145–180CrossRefGoogle Scholar
  3. Aravindraja C, Viszwapriya D, Pandian SK (2013) Ultradeep 16S rRNA sequencing analysis of geographically similar but diverse unexplored marine samples reveal varied bacterial community composition. PLoS One 8:e76724CrossRefGoogle Scholar
  4. Bolton JH, Fredrickson J, Elliott L, Metting F Jr (1992) Microbial ecology of the rhizosphere. Soil Microb Ecol 27:27–63Google Scholar
  5. Borum J, Sand JK, Binzer T, Pedersen O, Greve TM (2006) Oxygen movement in seagrasses. In: Seagrasses: biology, ecology and conservation. Springer, NetherlandsGoogle Scholar
  6. Brencic A, Winans SC (2005) Detection of and response to signals involved in host-microbe interactions by plant-associated bacteria. Microbiol Mol Biol Rev 69:155–194CrossRefGoogle Scholar
  7. Cebrian J, Duarte CM (2001) Detrital stocks and dynamics of the seagrass Posidonia oceanica (L.) Delile in the Spanish Mediterranean. Aquat Bot 70:295–309CrossRefGoogle Scholar
  8. Christiaen B, Mcdonald A, Cebrian J, Ortmann AC (2013) Response of the microbial community to environmental change during seagrass transplantation. Aquat Bot 109:31–38CrossRefGoogle Scholar
  9. Dietz H, Fischer M, Schmid B (1999) Demographic and genetic invasion history of a 9-year-old roadside population of Bunias orientalis L. (Brassicaceae). Oecologia 120:225–234CrossRefGoogle Scholar
  10. Escobar-Nino A, Luna C, Luna D, Marcos AT, Canovas D, Mellado E (2014) Selection and characterization of biofuel-producing environmental bacteria isolated from vegetable oil-rich wastes. PLoS One 9:e104063CrossRefGoogle Scholar
  11. Gallego S, Vila J, Nieto JM, Urdiain M, Rossello-Mora R, Grifoll M (2010) Breoghania corrubedonensis gen. nov. sp. nov., a novel alphaproteobacterium isolated from a Galician beach (NW Spain) after the Prestige fuel oil spill, and emended description of the family Cohaesibacteraceae and the species Cohaesibacter gelatinilyticus. Syst Appl Microbiol 33:316–321CrossRefGoogle Scholar
  12. Grime JP et al (2000) The response of two contrasting limestone grasslands to simulated climate change. Science 289:762–765CrossRefGoogle Scholar
  13. Hamisi MI, Lyimo TJ, Muruke M (2007) Cyanobacterial occurrence and diversity in seagrass meadows in coastal Tanzania. West Indian Ocean J Mar Sci 3:113–122Google Scholar
  14. Hamisi MI, Lyimo TJ, Muruke MH, Bergman B (2009) Nitrogen fixation by epiphytic and epibenthic diazotrophs associated with seagrass meadows along the Tanzanian coast, Western Indian Ocean. Aquat Microb Ecol 57:33–42CrossRefGoogle Scholar
  15. Hirano SS, Upper CD (2000) Bacteria in the leaf ecosystem with emphasis on pseudomonas syringae—a pathogen, ice nucleus, and epiphyte. Microbiol Mol Biol Rev 64:624–653CrossRefGoogle Scholar
  16. Holmer M, Bondgaard EJ (2001) Photosynthetic and growth response of eelgrass to low oxygen and high sulfide concentrations during hypoxic events. Aquat Bot 70:29–38CrossRefGoogle Scholar
  17. Jensen SI, Kuhl M, Glud RN, Jørgensen LB, Prieme A (2005) Oxic microzones and radial oxygen loss from roots of Zostera marina. Mar Ecol Prog Ser 293:49–58CrossRefGoogle Scholar
  18. Jose PA (2014) Molecular phylogeny and plant growth promoting traits of endophytic bacteria isolated from roots of seagrass Cymodocea serrulata. Indian J Med Sci 43:571–579Google Scholar
  19. Kim OS et al (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721CrossRefGoogle Scholar
  20. Kumar PS, Khan AB (2013) The distribution and diversity of benthic macroinvertebrate fauna in Pondicherry mangroves, India. Aquat Biosyst 9:15CrossRefGoogle Scholar
  21. Kurtz JC, Yates DF, Macauley JM, Quarles RL, Genthner FJ, Chancy CA, Devereux R (2003) Effects of light reduction on growth of the submerged macrophyte Vallisneria americana and the community of root-associated heterotrophic bacteria. J Exp Mar Biol Ecol 291:199–218CrossRefGoogle Scholar
  22. Lavery PS, Mateo MA, Serrano O, Rozaimi M (2013) Variability in the carbon storage of seagrass habitats and its implications for global estimates of blue carbon ecosystem service. PLoS One 8:e73748CrossRefGoogle Scholar
  23. Leon FD et al (2011) Phenotypic characteristics of isolates of aspergillus section Fumigati from different geographic origins and their relationships with genotypic characteristics. BMC Infect Dis 11:116CrossRefGoogle Scholar
  24. Loreau M et al (2001) Biodiversity and ecosystem functioning: current knowledge and future challenges. Science 294:804–808CrossRefGoogle Scholar
  25. Milbrandt EC, Greenawalt-Boswell J, Sokoloff PD (2008) Short-term indicators of seagrass transplant stress in response to sediment bacterial community disruption. Bot Mar 51:103–111CrossRefGoogle Scholar
  26. Moriarty D, Pollard P (1982) Diel variation of bacterial productivity in seagrass (Zostera capricorni) beds measured by rate of thymidine incorporation into DNA. Mar Biol 72:165–173CrossRefGoogle Scholar
  27. Moulin L, Munive A, Dreyfus B, Boivin-Masson C (2001) Nodulation of legumes by members of the β-subclass of Proteobacteria. Nature 411:948–950CrossRefGoogle Scholar
  28. Nielsen JT, Liesack W, Finster K (1999) Desulfovibrio zosterae sp. nov., a new sulfate reducer isolated from surface-sterilized roots of the seagrass Zostera marina. Int J Syst Bacteriol 49:859–865CrossRefGoogle Scholar
  29. Nubel U et al (1996) Sequence heterogeneities of genes encoding 16S rRNAs in Paenibacillus polymyxa detected by temperature gradient gel electrophoresis. J Bacteriol 178:5636–5643Google Scholar
  30. Pandey S, Sree A, Dash SS, Sethi DP, Chowdhury L (2013) Diversity of marine bacteria producing beta-glucosidase inhibitors. Microb Cell Fact. doi: 10.1186/1475-2859-12-35 Google Scholar
  31. Smith AC, Kostka JE, Devereux R, Yates DF (2004) Seasonal composition and activity of sulfate-reducing prokaryotic communities in seagrass bed sediments. Aquat Microb Ecol 37:183–195CrossRefGoogle Scholar
  32. Sondi I, Salopek-Sondi B (2004) Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. J Colloid Interface Sci 275:177–182CrossRefGoogle Scholar
  33. Sundqvist MK, Sanders NJ, Wardle DA (2013) Community and ecosystem responses to elevational gradients: processes, mechanisms, and insights for global change. Annu Rev Ecol Evol Syst 44:261–280CrossRefGoogle Scholar
  34. Tolli JD, Sievert SM, Taylor CD (2006) Unexpected diversity of bacteria capable of carbon monoxide oxidation in a coastal marine environment, and contribution of the Roseobacter-associated clade to total CO oxidation. Appl Environ Microbiol 72:1966–1973CrossRefGoogle Scholar
  35. Uku J, Bjork M, Bergman B, Diez B (2007) Characterization and comparison of prokaryotic epiphytes associated with three east african seagrasses. J Phycol 43:768–779CrossRefGoogle Scholar
  36. Wang DR et al (2012) Distribution of sea-grass resources and existing threat in Hainan Island. Mar Environ Sci 1:008Google Scholar
  37. Weidner S, Arnold W, Stackebrandt E, Pühler A (2000) Phylogenetic analysis of bacterial communities associated with leaves of the seagrass Halophila stipulacea by a culture-independent small-subunit rRNA gene approach. Microb Ecol 39:22–31CrossRefGoogle Scholar
  38. Weisburg WG, Barns SM, Pelletier DA, Lane DJ (1991) 16s ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703Google Scholar
  39. Welsh DT (2000) Nitrogen fixation in seagrass meadows: regulation, plant–bacteria interactions and significance to primary productivity. Ecol Lett 3:58–71CrossRefGoogle Scholar
  40. Yang D, Huang DJ (2011) Impacts of Typhoons Tianying and Dawei on seagrass distribution in Xincun Bay, Hainan Province, China. Acta Oceanol Sin 30:32–39CrossRefGoogle Scholar
  41. Yang D, Yang C (2009) Detection of seagrass distribution changes from 1991 to 2006 in Xincun Bay, Hainan, with satellite remote sensing. Sensors 9:830–844CrossRefGoogle Scholar
  42. Yu ZQ, Deng H, Wu KW, Du J, Ma M (2012) Nutrient contents of dominant seagrass species and their affecting factors in Hainan Province. J East China Normal Univ (Nat Sci) 4:131–141Google Scholar

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhouChina
  2. 2.Tropical Marine Biological Research Station in Hainan, South China Sea Institute of OceanologyChinese Academy of SciencesSanyaChina
  3. 3.State Key Laboratory of Tropical Oceanography, South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhouChina
  4. 4.University of Chinese Academy of SciencesBeijingChina

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