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Ecotoxicology

, Volume 24, Issue 7–8, pp 1478–1485 | Cite as

Spatial and vertical distribution of bacterial community in the northern South China Sea

  • Fu-Lin Sun
  • You-Shao WangEmail author
  • Mei-Lin Wu
  • Cui-Ci Sun
  • Hao Cheng
Article

Abstract

Microbial communities are highly diverse in coastal oceans and response rapidly with changing environments. Learning about this will help us understand the ecology of microbial populations in marine ecosystems. This study aimed to assess the spatial and vertical distributions of the bacterial community in the northern South China Sea. Multi-dimensional scaling analyses revealed structural differences of the bacterial community among sampling sites and vertical depth. Result also indicated that bacterial community in most sites had higher diversity in 0–75 m depths than those in 100–200 m depths. Bacterial community of samples was positively correlation with salinity and depth, whereas was negatively correlation with temperature. Proteobacteria and Cyanobacteria were the dominant groups, which accounted for the majority of sequences. The α-Proteobacteria was highly diverse, and sequences belonged to Rhodobacterales bacteria were dominant in all characterized sequences. The current data indicate that the Rhodobacterales bacteria, especially Roseobacter clade are the diverse group in the tropical waters.

Keywords

Northern South China Sea Bacterial community Rhodobacterales DGGE 

Notes

Acknowledgments

This research was supported by the National Natural Science Foundation of China (41406130, 41176101 and 41430966), the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA10020225), the key projects in the National Science & Technology Pillar Program in the Eleventh Five-year Plan Period (2012BAC07B0402) and State Key Laboratory of Tropical Oceanography (No.LTOZZ1402). We also thank the open cruises of the South China Sea Institute of Oceanology in 2011.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10646_2015_1472_MOESM1_ESM.docx (156 kb)
Supplementary material 1 (DOCX 156 kb). Comparison of the microbial diversity of sampling sites in the northern South China Sea

References

  1. Allgaier M, Uphoff H, Felske A, Wagner-Döbler I (2003) Aerobic anoxygenic photosynthesis in roseobacter clade bacteria from diverse marine habitats. Appl Environ Microbiol 69:5051–5059CrossRefGoogle Scholar
  2. Buchan A, González JM, Moran MA (2005) Overview of the marine roseobacter lineage. Appl Environ Microbiol 71:5665–5677CrossRefGoogle Scholar
  3. Calvo-Díaz A, Morán XAG, Nogueira E, Bode A, Varela M (2004) Picoplankton community structure along the Northern Iberian continental margin in late winter–early spring. J Plankton Res 26:1069–1081CrossRefGoogle Scholar
  4. Church MJ (2008) Resource control of bacterial dynamics in the sea. Microbial ecology of the oceans, 2nd edn. Wiley, Hoboken, pp 335–382CrossRefGoogle Scholar
  5. Dang H, Lovell CR (2002) Numerical dominance and phylotype diversity of marine Rhodobacter species during early colonization of submerged surfaces in coastal marine waters as determined by 16S ribosomal DNA sequence analysis and fluorescence in situ hybridization. Appl Environ Microbiol 68:496–504CrossRefGoogle Scholar
  6. Du J, Xiao K, Li L, Ding X, Liu H, Lu Y, Zhou S (2013) Temporal and spatial diversity of bacterial communities in coastal waters of the South China Sea. PLoS One 8:e66968CrossRefGoogle Scholar
  7. Fuhrman J (1992) Bacterioplankton roles in cycling of organic matter: the microbial food web. Primary productivity and biogeochemical cycles in the sea. Plenum Press, New York, pp 361–383CrossRefGoogle Scholar
  8. Fuhrman JA, Hewson I, Schwalbach MS, Steele JA, Brown MV, Naeem S (2006) Annually reoccurring bacterial communities are predictable from ocean conditions. Proc Natl Acad Sci 103:13104–13109CrossRefGoogle Scholar
  9. Giebel H-A, Brinkhoff T, Zwisler W, Selje N, Simon M (2009) Distribution of Roseobacter RCA and SAR11 lineages and distinct bacterial communities from the subtropics to the Southern Ocean. Environ Microbiol 11:2164–2178CrossRefGoogle Scholar
  10. Gilbert JA, Field D, Swift P, Newbold L, Oliver A, Smyth T, Somerfield PJ, Huse S, Joint I (2009) The seasonal structure of microbial communities in the Western English Channel. Environ Microbiol 11:3132–3139CrossRefGoogle Scholar
  11. Graff JR, Rines JEB, Donaghay PL (2011) Bacterial attachment to phytoplankton in the pelagic marine environment. Mar Eco-Prog Ser 441:15–24CrossRefGoogle Scholar
  12. Hodgson DA (2002) In: Whitton BA and Potts M (ed) The ecology of cyanobacteria: their diversity in time and space. J Paleolimn 28:383–384Google Scholar
  13. Legendre L, Rivkin RB (2008) Planktonic food webs: microbial hub approach. Mar Ecol-Prog Ser 365:289–309CrossRefGoogle Scholar
  14. Ling J, Dong J-D, Wang Y-S, Zhang Y-Y, Deng C, Lin L, Wu M-L, Sun F-L (2012) Spatial variation of bacterial community structure of the northern South China Sea in relation to water chemistry. Ecotoxicology 21:1669–1679CrossRefGoogle Scholar
  15. Longnecker K, Sherr B, Sherr E (2006) Variation in cell-specific rates of leucine and thymidine incorporation by marine bacteria with high and with low nucleic acid content off the Oregon coast. Aquat Microb Ecol 43:113–125CrossRefGoogle Scholar
  16. Lu Z, Gan J, Dai M, Cheung AY (2010) The influence of coastal upwelling and a river plume on the subsurface chlorophyll maximum over the shelf of the northeastern South China Sea. J Mar Syst 82:35–46CrossRefGoogle Scholar
  17. Moran M, Belas R, Schell M, Gonzalez J, Sun F, Sun S, Binder B, Edmonds J, Ye W, Orcutt B (2007) Ecological genomics of marine roseobacters. Appl Environ Microbiol 73:4559–4569CrossRefGoogle Scholar
  18. Morris RM, Vergin KL, Cho J-C, Rappé MS, Carlson CA, Giovannoni SJ (2005) Temporal and spatial response of bacterioplankton lineages to annual convective overturn at the Bermuda Atlantic time-series study site. Limnol Oceanogr 50:1687–1696CrossRefGoogle Scholar
  19. Muyzer G, Ramsing N (1995) Molecular methods to study the organization of microbial communities. Water Sci Technol 32:1–9CrossRefGoogle Scholar
  20. Nedwell D, Rutter M (1994) Influence of temperature on growth rate and competition between two psychrotolerant Antarctic bacteria: low temperature diminishes affinity for substrate uptake. Appl Environ Microbiol 60:1984–1992Google Scholar
  21. Ning X-r, Chai F, Xue H, Cai Y, Liu C, Shi J (2004) Physical biological oceanographic coupling influencing phytoplankton and primary production in the South China Sea. J Geophys Res 109Google Scholar
  22. Pan L, Zhang J, Chen Q, Deng B (2006) Picoplankton community structure at a coastal front region in the northern part of the South China Sea. J Plankton Res 28:337–343CrossRefGoogle Scholar
  23. Rooney-Varga JN, Giewat MW, Savin MC, Sood S, LeGresley M, Martin JL (2005) Links between phytoplankton and bacterial community dynamics in a coastal marine environment. Microb Ecol 49:163–175CrossRefGoogle Scholar
  24. Schäfer H, Muyzer G (2001) Denaturing gradient gel electrophoresis in marine microbial ecology. Methods Microbiol 30:425–468CrossRefGoogle Scholar
  25. Selje N, Simon M, Brinkhoff T (2004) A newly discovered Roseobacter cluster in temperate and polar oceans. Nature 427:445–448CrossRefGoogle Scholar
  26. Sherr E, Sherr B (1988) Role of microbes in pelagic food webs: a revised concept. Limnol Oceanogr 33:1225–1227CrossRefGoogle Scholar
  27. Sherr E, Sherr BF, Cowles TJ (2001) Mesoscale variablility in bacterial activity in the Northeast Pacific Ocean off Oregon. USA 25:21–30Google Scholar
  28. Sun FL, Wang YS, Wu ML, Jiang ZY, Sun CC, Chen H (2014) Genetic diversity of bacterial communities and gene transfer agents in northern South China Sea. PLoS One 9(11):e111892CrossRefGoogle Scholar
  29. Suzuki M, Giovannoni S (1996) Bias caused by template annealing in the amplification of mixtures of 16S rRNA genes by PCR. Appl Environ Microbiol 62:625–630Google Scholar
  30. Wagner-Döbler I, Biebl H (2006) Environmental biology of the marine roseobacter lineage. Annu Rev Microbiol 60:255–280CrossRefGoogle Scholar
  31. White PA, Kalff J, Rasmussen JB, Gasol JM (1991) The effect of temperature and algal biomass on bacterial production and specific growth rate in freshwater and marine habitats. Microb Ecol 21:99–118CrossRefGoogle Scholar
  32. Yin K (2002) Monsoonal influence on seasonal variations in nutrients and phytoplankton biomass in coastal waters of Hong Kong in the vicinity of the Pearl River estuary. Mar Ecol-Prog Ser 245:111–122CrossRefGoogle Scholar
  33. Zubkov MV, Sleigh MA, Burkill PH, Leakey RJ (2000) Picoplankton community structure on the Atlantic Meridional Transect: a comparison between seasons. Prog Oceanogr 45:369–386CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Fu-Lin Sun
    • 1
    • 2
  • You-Shao Wang
    • 1
    • 2
    Email author
  • Mei-Lin Wu
    • 1
  • Cui-Ci Sun
    • 1
    • 2
  • Hao Cheng
    • 1
  1. 1.State Key Laboratory of Tropical Oceanography, South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhouChina
  2. 2.Daya Bay Marine Biology Research Station, South China Sea Institute of OceanologyChinese Academy of SciencesShenzhenChina

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