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Niche specificity of ammonia-oxidizing archaeal and bacterial communities in a freshwater wetland receiving municipal wastewater in Daqing, Northeast China

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Abstract

Ecophysiological differences between ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) enable them to adapt to different niches in complex freshwater wetland ecosystems. The community characters of AOA and AOB in the different niches in a freshwater wetland receiving municipal wastewater, as well as the physicochemical parameters of sediment/soil samples, were investigated in this study. AOA community structures varied and separated from each other among four different niches. Wetland vegetation including aquatic macrophytes and terrestrial plants affected the AOA community composition but less for AOB, whereas sediment depths might contribute to the AOB community shift. The diversity of AOA communities was higher than that of AOB across all four niches. Archaeal and bacterial amoA genes (encoding for the alpha-subunit of ammonia monooxygenases) were most diverse in the dry-land niche, indicating O2 availability might favor ammonia oxidation. The majority of AOA amoA sequences belonged to the Soil/sediment Cluster B in the freshwater wetland ecosystems, while the dominant AOB amoA sequences were affiliated with Nitrosospira-like cluster. In the Nitrosospira-like cluster, AOB amoA gene sequences affiliated with the uncultured ammonia-oxidizing beta-proteobacteria constituted the largest portion (99 %). Moreover, independent methods for phylogenetic tree analysis supported high parsimony bootstrap values. As a consequence, it is proposed that Nitrosospira-like amoA gene sequences recovered in this study represent a potentially novel cluster, grouping with the sequences from Gulf of Mexico deposited in the public databases.

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Acknowledgments

This research was supported by a studentship from the Graduate School of The University of Hong Kong (KHL), additional financial support of this project was from Environmental Toxicology Education and Research Fund of this laboratory, and National Natural Science Foundation of China (41003031, 41273109, 51378208) and Shanghai Rising-Star Program (12QA1400800), and Fok Ying Tung Education Foundation (141077) (HL). We would like to thank Ms. Jessie Lai and Kelly Lau for support in chemical analysis and Dr. Jing Wang and Mr. Hongshuang Jiao for assistance in field sampling.

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The authors have no conflict of interest in research results report here.

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Authors and Affiliations

  1. Laboratory of Environmental Microbiology and Toxicology, School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong SAR, People’s Republic of China

    Kwok-Ho Lee & Ji-Dong Gu

  2. Laboratory of Microbial Ecology, Guangdong Academy of Forestry, No. 233, Guangshan 1st Road, Guangzhou, People’s Republic of China

    Yong-Feng Wang

  3. State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resource and Environmental Engineering, East China University of Science and Technology, Shanghai, 200237, People’s Republic of China

    Hui Li

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  1. Kwok-Ho Lee
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Correspondence to Ji-Dong Gu.

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Kwok-Ho Lee and Yong-Feng Wang were contributed to this work equally.

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Lee, KH., Wang, YF., Li, H. et al. Niche specificity of ammonia-oxidizing archaeal and bacterial communities in a freshwater wetland receiving municipal wastewater in Daqing, Northeast China. Ecotoxicology 23, 2081–2091 (2014). https://doi.org/10.1007/s10646-014-1334-3

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