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Perchlorate-induced oxidative stress in isolated liver mitochondria

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Abstract

As a new threat to environment all through the world, perchlorate (ClO4 −) was predominantly a thyrotoxin, and its toxic manifestations in non-thyroid were also documented. To date, little is known about the effect of ClO4 − on cell and organelle. To reveal the toxicity of ClO4 − on living organism in-depth, mitochondria isolated from liver of Carassius auratus were incubated with different concentrations of ClO4 −. The results demonstrated that ClO4 −-induced mitochondrial oxidative stress, and subsequently caused a gradual opening of permeability transition pore leading to mitochondrial swelling and lipid peroxidative membrane damage. ClO4 − has a conspicuous inhibition of electron transport chain activity which largely correlated to complexes I and IV. The investigations clearly demonstrated the oxidative stress of ClO4 − in mitochondria, may well reveal cytotoxic effects in vitro that merit further investigation.

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References

  • Albayrak T, Scherhammer V, Schoenfeld N, Braziulis E, Mund T, Bauer MKA, Scheffler IE, Grimm S (2003) The tumor suppressor cybl, a component of the respiratory chain, mediates apoptosis induction. Mol Biol Cell 14:3082–3096

    Article  CAS  Google Scholar 

  • Attanasio R, Scinicariello F, Blount BC, Valentin-Blasini L, Rogers KA, Nguyen DC, Murray HE (2011) Pendrin mediates uptake of perchlorate in a mammalian in vitro system. Chemosphere 84:1484–1488

    Article  CAS  Google Scholar 

  • Baldridge MG, Stahl RL, Gerstenberger SL, Tripoli V, Hutz RJ (2004) In utero and lactational exposure of Long-Evans rats to ammonium perchlorate (AP) disrupts ovarian follicle maturation. Reprod Toxicol 19:155–161

    Article  CAS  Google Scholar 

  • Borjan M, Marcella S, Blount B, Greenberg M, Zhang J, Murphy E, Valentin-Blasini L, Robson M (2011) Perchlorate exposure in lactating women in an urban community in New Jersey. Sci Total Environ 409:460–464

    Article  CAS  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  Google Scholar 

  • Bradford CM, Rinchard J, Carr JA, Theodorakis C (2005) Perchlorate affects thyroid function in eastern mosquitofish (Gambusia holbrooki) at environmentally relevant concentrations. Environ Sci Technol 39:5190–5195

    Article  CAS  Google Scholar 

  • Cañas JE, Cheng QQ, Tian K, Anderson TA (2006) Optimization of operating conditions for the determination of perchlorate in biological samples using preconcentration/preelution ion chromatography. J Chromatogr A 1103:102–109

    Article  Google Scholar 

  • Cela O, Piccoli C, Scrima R, Quarato G, Marolla A, Cinnella G, Dambrosio M, Capitanio N (2010) Bupivacaine uncouples the mitochondrial oxidative phosphorylation, inhibits respiratory chain complexes I and III enhances ROS production: results of a study on cell cultures. Mitochondrion 10:487–496

    Article  CAS  Google Scholar 

  • Dong YL, Zhou PJ, Jiang SY, Pan XW, Zhao XH (2009) Induction of oxidative stress and apoptosis by pentachlorophenol in primary cultures of Carassius carassius hepatocytes. Comp Biochem Phys C 150:179–185

    Google Scholar 

  • Dröse S, Brandt U (2008) The mechanism of mitochondrial superoxide production by the cytochrome bc(1) complex. J Biol Chem 283:21649–21654

    Article  Google Scholar 

  • Feng MB, Li Y, Qu RJ, Wang LS, Wang ZY (2013) Oxidative stress biomarkers in freshwater fish Carassius auratus exposed to decabromodiphenyl ether and ethane, or their mixture. Ecotoxicology 22:1101–1110

    Article  CAS  Google Scholar 

  • Houtkooper RH, Vaz FM (2008) Cardiolipin, the heart of mitochondrial metabolism. Cell Mol Life Sci 65:2493–2506

    Article  CAS  Google Scholar 

  • Indo HP, Davidson M, Yen HC, Suenaga S, Tomita K, Nishii T, Higuchi M, Koga Y, Ozawa T, Majima HJ (2007) Evidence of ROS generation by mitochondria in cells with impaired electron transport chain and mitochondrial DNA damage. Mitochondrion 7:106–118

    Article  CAS  Google Scholar 

  • Jezek P, Hlavata L (2005) Mitochondria in homeostasis of reactive oxygen species in cell, tissues, and organism. Int J Biochem Cell Biol 37:2478–2503

    Article  CAS  Google Scholar 

  • Lemarie A, Grimm S (2009) Mutations in the heme b-binding residue of SDHC inhibit assembly of respiratory chain complex II in mammalian cells. Mitochondrion 9:254–260

    Article  CAS  Google Scholar 

  • Li HP, Feng T, Shi XY, Liang P, Zhang Q, Gao XW (2009) Effects of pyrethroids and endosulfan on fluidity of mitochondria membrane in Chilo suppressalis (Walker). Pestic Biochem Phys 95:72–76

    Article  CAS  Google Scholar 

  • Liu YW, Liu Y, Wang CX, Qu SS, Deng FJ (2000) Microcalorimetric studies of the inhibition of sodium azide on the mitochondrial metabolism of fish liver tissue. Thermochim Acta 351:51–54

    Article  CAS  Google Scholar 

  • Liu Y, Zhou QX, Xie XJ, Lin DS, Dong LX (2010) Oxidative stress and DNA damage in the earthworm Eisenia fetida induced by toluene, ethylbenzene and xylene. Ecotoxicology 19:1551–1559

    Article  CAS  Google Scholar 

  • Muramatsu Y, Maemoto T, Washita A, Matsuoka N (2007) Novel neuroprotective compound SCH-20148 rescues thymocytes and SH-SY5Y cells from thapsigargin-induced mitochondrial membrane potential reduction and cell death. Eur J Pharmacol 563:40–48

    Article  CAS  Google Scholar 

  • Murray CW, Egan SK, Kim H, Beru N, Bolger PM (2008) US food and drug administration’s total diet study: dietary intake of perchlorate and iodine. J Expo Sci Environ Epidemiol 18:571–580

    Article  CAS  Google Scholar 

  • Mustafa SA, Al-Subiai SN, Davies S, Jha AN (2011) Hypoxia-induced oxidative DNA damage links with higher level biological effects including specific growth rate in common carp, Cyprinus carpio L. Ecotoxicology 20:1455–1466

    Article  CAS  Google Scholar 

  • Niki E, Yoshida Y, Saito Y, Noguchi N (2005) Lipid peroxidation: mechanism, inhibition, and biological effects. Biochem Biophys Res Commun 338:668–676

    Article  CAS  Google Scholar 

  • Ohnishi T, Ohnishi ST, Shinzawa-Ito K, Yoshikawa S (2008) Functional role of coenzyme Q in the energy coupling of NADH-CoQ oxidoreductase (complex I): stabilization of the semiquinone state with the application of inside-positive membranepotential to proteliposomes. BioFactors 32:13–22

    Article  CAS  Google Scholar 

  • Paranagama MP, Sakamoto K, Amino H, Awano M, Miyoshi H, Kita K (2010) Contribution of FAD and quinone binding sites to the production of reactive oxygen species from Ascaris suum mitochondrial complex II. Mitochondrion 10:158–165

    Article  CAS  Google Scholar 

  • Parihar MS, Parihar A, Fujita M, Hashimoto M, Ghafourifar P (2009) Alpha-synuclein overexpression and aggregation exacerbates impairment of mitochondrial functions by augmenting oxidative stress. Int J Biochem Cell Biol 41:2015–2024

    Article  CAS  Google Scholar 

  • Park JW, Rinchard J, Liu FJ, Anderson TA, Kendall RJ, Theodorakis CW (2006) The thyroid endocrine disruptor perchlorate affects reproduction, growth, and survival of mosquitofish. Ecotoxicol Environ Safe 63:343–352

    Article  CAS  Google Scholar 

  • Pearce EN, Leung AM, Blount BC, Bazrafshan HR, He X, Pino S, Valentin-Blasini L, Braverman LE (2007) Breast milk iodine and perchlorate using concentrations in lactating Boston-area women. J Clin Endocrinol Metab 92:1673–1677

    Article  CAS  Google Scholar 

  • Rustin P, Chretien D, Bourgeron T, Gerard B, Rötig A, Saudubray JM, Munnich A (1994) Biochemical and molecular investigations in respiratory chain deficiencies. Clin Chim Acta 228:35–51

    Article  CAS  Google Scholar 

  • Sanchez CA, Krieger RI, Valentin-Blasini L, Blount BC, Khandaker N (2006) Perchlorate accumulation and potential exposure from durum wheat irrigated with Colorado River water. J ASTM Int 3:704–710

    Article  Google Scholar 

  • Sanchez CA, Blount BC, Valentin-Blasini L, Lesch SM, Krieger RI (2008) Perchlorate in the feed-dairy continuum of the southwestern United States. J Agric Food Chem 56:5443–5450

    Article  CAS  Google Scholar 

  • Sen T, Sen N, Jana S, Khan FH, Chatterjee U, Chakrabarti S (2007) Depolarization and cardiolipin depletion in aged rat brain mitochondria: relationship with oxidative stress and electron transport chain activity. Neurochem Int 50:719–725

    Article  CAS  Google Scholar 

  • Shao B, Zhu LS, Miao D, Wang J, Wang JH, Xie H, Zhang QM, Du ZK, Zhu SY (2012) DNA damage and oxidative stress induced by endosulfan exposure in zebrafish (Danio rerio). Ecotoxicology 21:1533–1540

    Article  CAS  Google Scholar 

  • Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M (2006) Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem Biol Interact 160:1–40

    Article  CAS  Google Scholar 

  • Zhang FL, Xu ZF, Gao J, Xu B, Deng Y (2008) In vitro effect of manganese chloride exposure on energy metabolism and oxidative damage of mitochondria isolated from rate brain. Environ Toxicol Pharmacol 26:232–236

    Article  CAS  Google Scholar 

  • Zhao YB, Ye LH, Liu HX, Xia Q, Zhang Y, Yang XD, Wang K (2010) Vanadium compounds induced mitochondria permeability transition pore (PTP) opening related to oxidative stress. J Inorg Biochem 104:371–378

    Article  CAS  Google Scholar 

  • Zhao XH, Zhou PJ, Chen X, Dong YL, Jiang SY, Ding L (2011) Microcalorimetric studies of perchlorate on heat production by hepatocytes and mitochondria isolated from Carassius auratus. Chemosphere 83:422–428

    Article  CAS  Google Scholar 

  • Zhu JC, Li CH, Liu Y, Zhang ZH, Hou AX, Qu SS (2006) A microcalorimetric study of the action of mercuric chloride on the metabolism of mitochondria isolated from Cyprinus carpio liver tissue. J Therm Anal Calorim 83:181–186

    Article  CAS  Google Scholar 

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Acknowledgments

The authors gratefully acknowledge the financial supports from the National Hi-Tech. Research and Development, program (863) of China (No. 2007AA06Z418), the National Natural Science Foundation of China (Nos. 20577036, 20777058, 20977070).

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Hubei Biomass-Resource Chemistry and Environmental Biotechnology Key Laboratory, College of Resources and Environmental Sciences, Wuhan University, Wuhan, 430072, Hubei, China

    Xiaohu Zhao, Peijiang Zhou, Xiu Chen, Xi Li & Ling Ding

  2. Micro-element Research Center, Huazhong Agricultural University, Wuhan, 430070, China

    Xiaohu Zhao

Authors
  1. Xiaohu Zhao
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  2. Peijiang Zhou
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  3. Xiu Chen
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  4. Xi Li
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  5. Ling Ding
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Correspondence to Peijiang Zhou.

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Zhao, X., Zhou, P., Chen, X. et al. Perchlorate-induced oxidative stress in isolated liver mitochondria. Ecotoxicology 23, 1846–1853 (2014). https://doi.org/10.1007/s10646-014-1312-9

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  • DOI: https://doi.org/10.1007/s10646-014-1312-9

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