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The respiratory burst activity and expression of catalase in white shrimp, Litopenaeus vannamei, during long-term exposure to pH stress

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Abstract

In this study, changes of reactive oxygen species (ROS) and the mRNA expression of catalase of the Pacific white shrimp, Litopenaeus vannamei, exposed to pH (5.4, 6.7, 8.0, and 9.3) stress was investigated at different stress time (24, 48, 72, 96, and 120 h). Level of malondialdehyde (MDA) in shrimp also were assessed. The results revealed that acidic (pH 5.4 and 6.7) or alkaline exposure (pH 9.3) induced production of ROS hemocytes and increase of MDA level in shrimp. Moreover, the catalase mRNA expression in hepatopancreas of L. vannamei was up-regulated in 24 h at pH 5.4, in 72 h at pH 6.7 and in 48 h at pH 9.3, whereas was down-regulated significantly after 72 h acidic (pH 5.4 and 6.7) or alkaline (pH 9.4) exposure. In the present study, there was the relationship between ROS and catalase mRNA expression under normal acidic and alkaline conditions. At pH 8, the increase of catalase transcripts due to up-regulation by ROS, whereas MDA level did not significantly change, suggesting activation of corresponding protective mechanisms of detoxifying ROS is essential for the proper functioning of cells and the survival of shrimps.

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Acknowledgments

This research was supported by the National Natural Science Foundation of China (Grant nos. 30570287 and 30970455), and the Natural Science Foundation of the Guangdong Province in the P.R. China (Grant nos. 06025052 and 8151063101000035), and Guangdong Provincial Oceanic Fisheries Science and Technology Project (Grant no. A201001H02 and A200901H06). We are grateful for the help of Profs. Qili Feng and Sichun Zheng from the laboratory of Molecular Entomology, South China Normal University.

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  1. Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou, 510631, P.R. China

    Wei-Na Wang, Bao-Sheng Li, Jin-Jian Liu, Shi-Juan Su, Juan Wu, Lei Wang & An-Li Wang

  2. College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, 510631, P.R. China

    Lei Shi

  3. Department of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, KS, 66506, USA

    M. J. Alam

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  1. Wei-Na Wang
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Correspondence to Wei-Na Wang.

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Wang, WN., Li, BS., Liu, JJ. et al. The respiratory burst activity and expression of catalase in white shrimp, Litopenaeus vannamei, during long-term exposure to pH stress. Ecotoxicology 21, 1609–1616 (2012). https://doi.org/10.1007/s10646-012-0937-9

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