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Effects of alkali stress on growth, free amino acids and carbohydrates metabolism in Kentucky bluegrass (Poa pratensis)

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Abstract

Soil alkalization is one of the most prominent adverse environmental factors limiting plant growth, while alkali stress affects amino acids and carbohydrates metabolism. The objective of this study was conducted to investigate the effects of alkali stress on growth, amino acids and carbohydrates metabolism in Kentucky bluegrass (Poa pratensis). Seventy-day-old plants were subjected to four pH levels: 6.0 (control), 8.0 (low), 9.4 (moderate) and 10.3 (severe) for 7 days. Moderate to severe alkali stress (pH >9.4) caused a significant decline in turf quality and growth rate in Kentucky bluegrass. Soluble protein was unchanged in shoots, but decreased in roots as pH increased. The levels of amino acids was kept at the same level as control level at 4 days after treatment (DAT) in shoots, but greater at 7 DAT, when plants were subjected to severe (pH 10.3) alkali stress. The alkali stressed plants had a greater level of starch, water soluble carbohydrate and sucrose content, but lower level of fructose and glucose. Fructan and total non-structural carbohydrate (TNC) increased at 4 DAT and decreased at 7 DAT for alkali stressed plants. These results suggested that the decrease in fructose and glucose contributed to the growth reduction under alkali stress, while the increase in amino acids, sucrose and storage form of carbohydrate (fructan, starch) could be an adaptative mechanism in Kentucky bluegrass under alkali stress.

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References

  • Ashraf M, Harris PC (2004) Potential biochemical indicators of salinity tolerance in plants. Plant Sci 166:3–16

    Article  CAS  Google Scholar 

  • Barnett NM, Naylor A (1966) Amino acid and protein metabolism in Bermuda grass during water stress. Plant Physiol 41:1222–1230

    Article  CAS  Google Scholar 

  • Bie Z (2004) Effects of sodium sulfate and sodium bicarbonate on the growth, gas exchange and mineral composition of lettuce. Sci Hortic 99:215–224

    Article  CAS  Google Scholar 

  • Bohnert HJ, Su H, Shen B (1999) Molecular mechanisms of salinity tolerance. In: Shinozaki K, Yamaguchi-Shinozaki Y (eds) Molecular responses to cold, drought, heat and salt stress in higher plants. Landes Bioscience, Austin, pp 29–60

    Google Scholar 

  • Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  Google Scholar 

  • Bray EA, Bailey-Serres J, Weretilnyk E (2000) Responses to abiotic stresses. In: Buchanan BB, Gruissem W, Jones RL (eds) Biochemistry and molecular biology of plants. American Society of Plant Physiologists, Rockville, MD, pp 1158–1203

  • Cardarelli M, Rouphae Y, Rea E, Colla G (2010) Mitigation of alkaline stress by arbuscular mycorrhiza in zucchini plants grown under mineral and organic fertilization. J Plant Nutr Soil Sci 173:778–787

    Article  CAS  Google Scholar 

  • Clark RB, Zeto SK (1996) Mineral acquisition by mycorrhizal maize grown on acid and alkaline soil. Soil Biol Biochem 28:1495–1503

    Article  CAS  Google Scholar 

  • Clark GT, Zuther E, Outred HA, McManus MT, Heyer AG (2004) Tissue-specific changes in remobilisation of fructan in the xerophytic tussock species Festuca novae-zelandiae in response to a water deficit. Funct Plant Biol 31:377–389

    Article  CAS  Google Scholar 

  • De Gara L, De Pinto MC, Moliterni V, D’Egidio MG (2003) Redox regulation and storage processes during maturation in kernels of Triticum durum. J Exp Bot 54:249

    Article  Google Scholar 

  • Delory GE, King EJ (1945) A sodium carbonate-bicarbonate buffer for alkaline phosphatases. Biochem J 39:245

    CAS  Google Scholar 

  • Díaz P, Borsani O, Márquez A, Monza J (2005) Nitrogen metabolism in relation to drought stress responses in cultivated and model Lotus species. Lotus Newslett 35:83–92

    Google Scholar 

  • Downs CG, Somerfield SD, Davey MC (1997) Cytokinin treatment delays senescence but not sucrose loss in harvested broccoli. Postharvest Biol Technol 11:93–100

    Article  CAS  Google Scholar 

  • Dubey R (1997) Photosynthesis in plants under stressful conditions. In: Pessarakli M (ed) Handbook of photosynthesis. Marcel Dekker, New York, pp 859–875

    Google Scholar 

  • Fageria NK, Baligar VC (1999) Growth and nutrient concentrations of common bean, lowland rice, corn, soybean, and wheat at different soil ph on an inceptisol. J Plant Nutr 22:1495–1507

    Article  CAS  Google Scholar 

  • Farooq M, Wahid A, Lee D, Ito O, Siddque K (2009) Advance in drought resistance of rice. Crit Rev Plant Sci 28:199–217

    Article  CAS  Google Scholar 

  • Fu J, Dernoeden PH (2008) Carbohydrate metabolism in creeping bentgrass as influenced by two summer irrigation practices. J Am Soc Hortic Sci 133:678–683

    Google Scholar 

  • Gilbert GA, Gadush MV, Wilson C, Madore MA (1998) Amino acid accumulation in sink and source tissues of Coleus blumei Benth. during salinity stress. J Exp Bot 49:107–114

    CAS  Google Scholar 

  • Gill P, Sharma A, Singh P, Bhullar S (2001) Effect of various abiotic stresses on growth, soluble sugars and water relationships of sorghum seedlings grown in light and darkness. Bulg J Plant Physiol 27:72–84

    CAS  Google Scholar 

  • Greenway H, Munns R (1980) Mechanism of salt tolerance in nonhalophytes. Annu Rev Plant Physiol 31:149–190

    Article  CAS  Google Scholar 

  • Hoagland DR, Arnon DI (1950) The water-culture method for growing plants without soil. Calif Agric Exp Stat 347:1–32

    Google Scholar 

  • Hoch G, Popp M, Körner C (2002) Altitudinal increase of mobile carbon pools in Pinus cembra suggests sink limitation of growth at Swiss treeline. Oikos 98:361–374

    Article  CAS  Google Scholar 

  • Hsiao TC (1973) Plant responses to water deficit. Ann Rev Plant Physiol 24:519–570

    Article  CAS  Google Scholar 

  • Huang B, Liu L (2009) Physiological responses of creeping bentgrass to heat stress affected by phosphonate fungicide application. Intl Turfgrass Soc Res J 11:799–806

    Google Scholar 

  • Kafi M, Stewart W, Borland A (2003) Carbohydrate and proline contents in leaves, roots, and apices of salt-tolerant and salt-sensitive wheat cultivars. Russ J Plant Physiol 50:155–162

    Article  CAS  Google Scholar 

  • Kerepesi I, Galiba G (2000) Osmotic and salt stress-induced alteration in soluble carbohydrate content in wheat seedlings. Crop Sci 40:482–487

    Article  CAS  Google Scholar 

  • Kerkeb L, Krmer U (2003) The role of free histidine in xylem loading of nickel in Alyssum lesbiacum and Brassica juncea. Plant Physiol 131:716

    Article  CAS  Google Scholar 

  • Klotke J, Kopka J, Gatzke N, Heyer AG (2004) Impact of soluble sugar concentrations on the acquisition of freezing tolerance in accessions of Arabidopsis thaliana with contrasting cold adaptation evidence for a role of raffinose in cold acclimation. Plant Cell Environ 27:1395–1404

    Article  CAS  Google Scholar 

  • Krapp A, Stitt M (1995) An evaluation of direct and indirect mechanisms for the ‘sink-regulation’ of photosynthesis in spinach: changes in gas exchange, carbohydrates, metabolites, enzyme activities and steady-state transcript levels after cold-girdling source leaves. Planta 195:313

    Article  CAS  Google Scholar 

  • Lea PJ, Sodek L, Parry MAJ, Shewry PR, Halford NG (2007) Asparagine in plants. Ann Appl Biol 150:1–26

    Article  CAS  Google Scholar 

  • Less H, Galili G (2008) Principal transcriptional programs regulating plant amino acid metabolism in response to abiotic stresses. Plant Physiol 147:316–330

    Article  CAS  Google Scholar 

  • Li R, Volenec JJ, Joern BC, Cunningham SM (1996) Seasonal changes in nonstructural carbohydrates, protein, and macronutrients in roots of alfalfa, red clover, sweet clover, and birdsfoot trifoil. Crop Sci 36:617–623

    Article  CAS  Google Scholar 

  • Li Y, Shi D, Li Y, Wang Y (2002) Effect of complex alkali-saline stress on sorghum seedlings. Rain Fed Crops 22:41–45

    Google Scholar 

  • Livingston DP, Hincha DK, Heyer AG (2009) Fructan and its relationship to abiotic stress tolerance in plants. Cell Mol Life Sci 66:2007–2023

    Article  CAS  Google Scholar 

  • Mansour MMF (2000) Nitrogen containing compounds and adaptation of plants to salinity stress. Biol Plant 43:491–500

    Article  CAS  Google Scholar 

  • Marcum K (2008) Handbook of turfgrass management and physiology. CRC Press, Boca Raton

    Google Scholar 

  • Martino CD, Delfine S, Pizzuto R, Loreto F, Fuggi A (2003) Free amino acids and glycine betaine in leaf osmoregulation of spinach responding to increasing salt stress. New Phytol 158:455–463

    Article  Google Scholar 

  • Mayer R, Cherry J, Rhodes D (1990) Effects of heat shock on amino acid metabolism of cowpea cells. Plant Physiol 94:796–810

    Article  CAS  Google Scholar 

  • Mothes K (1940) Zur biosythese der säureamide asparagin und glutamin. Planta 30:726–756

    Article  CAS  Google Scholar 

  • Paradiso A, Cecchini C, De Gara L, D’Egidio MG (2006) Functional, antioxidant and rheological properties of meal from immature durum wheat. J Cereal Sci 43:216–222

    Article  CAS  Google Scholar 

  • Rai VK (2002) Role of amino acids in plant responses to stresses. Biol Plant 45:481–487

    Article  CAS  Google Scholar 

  • Rolland F, Baena-Gonzalez E, Sheen J (2006) Sugar sensing and signaling in plants: conserved and novel mechanisms. Annu Rev Plant Biol 57:675–709

    Article  CAS  Google Scholar 

  • Sasaki H, Ichimura K, Oda M (1996) Changes in sugar content during cold acclimation and deacclimation of cabbage seedlings. Ann Bot 78:365

    Article  CAS  Google Scholar 

  • Shi D, Sheng Y (2005) Effect of various salt-alkaline mixed stress conditions on sunflower seedlings and analysis of their stress factors. Environ Exp Bot 54:8–21

    Article  CAS  Google Scholar 

  • Shi D, Zhao K (1997) Effects of sodium chloride and carbonate on growth of puccinellia tenuiflora and on present state of mineral elements in nutrient solution. Acta Pratacult Sinica 6:51–61

    Google Scholar 

  • Smith D (1972) Carbohydrate reserves of grasses. Academic Press, London

    Google Scholar 

  • Stoop JMH, Pharr DM (1994) Growth substrate and nutrient salt environment alter mannitol-hexose partitioning in celery petioles. J Am Soc Hortic Sci 119:237

    CAS  Google Scholar 

  • Szabados L, Savouré A (2010) Proline: a multifunctional amino acid. Trends Plant Sci 15:89–97

    Article  CAS  Google Scholar 

  • Takahashi M, Nakanishi H, Kawasaki S, Nishizawa NK, Mori S (2001) Enhanced tolerance of rice to low iron availability in alkaline soils using barley nicotianamine aminotransferase genes. Nat Biotechnol 19:466–469

    Article  CAS  Google Scholar 

  • Unno H, Yamamoto S (2004) Effect of salt stress on water soluble carbohydrates in leaf sheaths of forage grasses. Grassland Sci 50:300–303

    CAS  Google Scholar 

  • Van den Ende W, Valluru R (2009) Sucrose, sucrosyl oligosaccharides, and oxidative stress: scavenging and salvaging? J Exp Bot 60:9–18

    Article  Google Scholar 

  • Yang J, Zhang J, Wang Z, Zhu Q, Liu L (2004) Activities of fructan-and sucrose-metabolizing enzymes in wheat stems subjected to water stress during grain filling. Planta 220:331–343

    Article  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, the Chinese Academy of Sciences, Wuhan, Hubei, 430074, People’s Republic of China

    Pingping Zhang, Jinmin Fu & Longxing Hu

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  1. Pingping Zhang
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  2. Jinmin Fu
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  3. Longxing Hu
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Correspondence to Longxing Hu.

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Zhang, P., Fu, J. & Hu, L. Effects of alkali stress on growth, free amino acids and carbohydrates metabolism in Kentucky bluegrass (Poa pratensis). Ecotoxicology 21, 1911–1918 (2012). https://doi.org/10.1007/s10646-012-0924-1

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