Abstract
We assessed the potential eco-toxicological risks of the herbicide acetochlor on fungal communities in the microcosm of black soil using 28S rRNA gene-PCR-DGGE and clone library analysis. The acetochlor was applied to black soil at four concentrations (0-control, 50, 150, and 250 mg/kg). The DGGE fingerprint patterns indicated that acetochlor stimulated fungal communities at day 7 after application, after which there was a suppression effect. The fungal communities in acetochlor-treated soil gradually became more like that of the control during the 60-day experimental period. Diversity indices in the 50 and 150 mg/kg acetochlor treatments changed more rapidly than in the 250 mg/kg acetochlor treatment. The cluster analysis indicated a significant change in fungal community structure after application of acetochlor. The impacts were markedly greater in the 150 and 250 mg/kg acetochlor treatments compared with the 50 mg/kg acetochlor treatment. Sequencing of clones showed that acetochlor application resulted in an increase in pathogenic and non-cultivatable fungal populations, which could increase the risk of plant disease outbreaks.
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This project was supported by the National Science Foundation for Young Scientists of China (No. 40701088) and the Knowledge Innovation Project of the Chinese Academy of Science (KZCX2-YW-445).
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Xin-Yu, L., Zhen-Cheng, S., Xu, L. et al. Assessing the effects of acetochlor on soil fungal communities by DGGE and clone library analysis. Ecotoxicology 19, 1111–1116 (2010). https://doi.org/10.1007/s10646-010-0493-0
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DOI: https://doi.org/10.1007/s10646-010-0493-0