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Bioaccumulation and ROS generation in Coontail Ceratophyllum demersum L. exposed to phenanthrene

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Abstract

Phenanthrene bioaccumulation, induction free radicals and their consequent biochemical responses in coontail (Ceratophyllum demersum L.) were examined. Plants were exposed to different levels (0.01, 0.02, 0.05, 0.07 and 0.1 mg/l) of phenanthrene for 10 days. Results showed that the phenanthrene concentration in the plants was exponentially correlated to exposure concentration (R 2 = 0.958) and phenanthrene exposure significantly increased the total free radicals and superoxide anion in the plants. The activities of antioxidant enzymes and the contents of glutathione were determined. The superoxide dismutase (SOD) activity and reduced glutathione (GSH) content were inhibited, while the catalase (CAT), peroxidase (POD), glutathione-s-transferase (GST) activities and oxidized glutathione (GSSG) content were significantly induced. Changes in the contents of chlorophyll and malondialdehyde (MDA) indicated that the MDA content was enhanced after phenanthrene exposure and the contents of chlorophyll were significantly increased in the 0.01 mg/l group. These experimental data demonstrated that the bioaccumulation of phenanthrene induced the production of free radicals and ROS, and changed the antioxidant defense system, ultimately resulting in oxidative damage in C. demersum.

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Acknowledgments

We thank Yunxia Sui for analysis of EPR spectra. This work was supported by the National Fundamental Research Foundation of China (2009CB421604) and Natural Science Foundation of China (40902067).

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Authors and Affiliations

  1. State Key Laboratory of Pollution Control and Resources Reuse, School of Environment, Nanjing University, Nanjing, 210093, China

    Ying Yin, Xiaorong Wang, Liuyan Yang & Hongyan Guo

  2. Department of Hydrosciences, Nanjing University, Nanjing, 210093, China

    Yuanyuan Sun

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  1. Ying Yin
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  2. Xiaorong Wang
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Correspondence to Xiaorong Wang.

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Yin, Y., Wang, X., Yang, L. et al. Bioaccumulation and ROS generation in Coontail Ceratophyllum demersum L. exposed to phenanthrene. Ecotoxicology 19, 1102–1110 (2010). https://doi.org/10.1007/s10646-010-0492-1

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