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Investigation of the fate and effects of acetyl cedrene on Capitella teleta and sediment bacterial community

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Abstract

The fate of the fragrance material, acetyl cedrene (AC), in sediment was examined in a 16 day laboratory experiment using the sediment microbial community subjected to the following treatments: AC (nominal concentration; 0 and 50 μg g−1 dw sediment) and macrofaunal worms (with/without Capitella teleta (formerly Capitella sp. I)). Furthermore effects of AC on microbial respiration in the system were determined by examining CO2 flux. T-RFLP (terminal restriction fragment length polymorphism) was used to analyze PCR (polymerase chain reaction) amplified 16S DNA gene fragments from the sediments to detect changes in the structure and diversity of the bacterial community. In addition, survival of C. teleta in sediment was determined. Lastly, we examined how the interactions between microbes and C. teleta in the sediment affected the above-mentioned parameters. The results showed that there was an interaction between worm treatment and time of sampling on the loss of AC from the sediment. This was caused by AC loss initially being fastest in the sediment with C. teleta present, but at experimental termination there was no significant difference between the two treatments (i.e., with/without worms) in the amount of AC remaining in the sediment. Survival of C. teleta was significantly reduced by AC at experimental termination, but neither microbial respiration nor structure and diversity of the bacterial community were significantly affected.

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Acknowledgments

The authors thank K. Vestberg for assistance with the molecular work and P. Christensen for assistance with the fragrance extraction method. Our thanks also go to G. Banta and A.B. Faarborg for guidance on the CO2 measurement. Lastly, we are grateful to L.D. Rasmussen, National Veterinary Institute, DTU for running the T-RFLP analysis. This work was partly funded by a grant from the Research Institute for Fragrance Materials.

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  1. Department of Microbiology, Institute of Biology, Copenhagen University, Sølvgade 83H, 1307, Copenhagen K, Denmark

    Lea Ellegaard-Petersen & Anders Priemé

  2. Department of Environmental, Social and Spatial Change, Roskilde University, PO BOX 260, Universitetsvej 1, 4000, Roskilde, Denmark

    Henriette Selck & Valery Forbes

  3. Research Institute for Fragrance Materials, 50 Tice Boulevard, Woodcliff Lake, NJ, 07677, USA

    Daniel Salvito

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  1. Lea Ellegaard-Petersen
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  2. Henriette Selck
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  5. Valery Forbes
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Correspondence to Lea Ellegaard-Petersen.

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Ellegaard-Petersen, L., Selck, H., Priemé, A. et al. Investigation of the fate and effects of acetyl cedrene on Capitella teleta and sediment bacterial community. Ecotoxicology 19, 1046–1058 (2010). https://doi.org/10.1007/s10646-010-0486-z

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