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Correlation between an in vitro and an in vivo measure of dioxin sensitivity in birds

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Abstract

We describe a statistically significant correlation between two well-characterized responses to dioxin-like compounds in birds; induction of 7-ethoxyresorufin-O-deethylase (EROD) activity in cultured hepatocytes, and embryo mortality. Data were obtained from a review of the literature. EROD EC50 values for 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and 6 polychlorinated biphenyls (PCBs) were strongly correlated with LD50 values in chicken embryos (r 2 = 0.93, P < 0.005). Similarly, EROD EC50 values for TCDD and a potent dioxin-like compound, PCB 126, were correlated with embryonic LD50 values in different species of birds (chicken, ring-necked pheasant, turkey, double-crested cormorant, and common tern) (r 2 = 0.92, P < 0.005). Our findings contribute to a developing understanding of the molecular basis for differential dioxin sensitivity in birds, and validate the EROD bioassay as a useful predictive tool for ecological risk assessment.

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Acknowledgments

This work was funded in part by Environment Canada’s Wildlife Toxicology and Disease program.

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Correspondence to Jessica A. Head.

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Head, J.A., Kennedy, S.W. Correlation between an in vitro and an in vivo measure of dioxin sensitivity in birds. Ecotoxicology 19, 377–382 (2010). https://doi.org/10.1007/s10646-009-0421-3

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