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Inorganic mercury exposure: toxicological effects, oxidative stress biomarkers and bioaccumulation in the tropical freshwater fish matrinxã, Brycon amazonicus (Spix and Agassiz, 1829)

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Abstract

Alterations in the antioxidant cellular system have often been proposed as biomarkers of pollutant-mediated toxicity. This study evaluated the effects of mercury on oxidative stress biomarkers and bioaccumulation in the liver, gills, white muscle and heart of the freshwater fish matrinxã, Brycon amazonicus, exposed to a nominal and sub-lethal concentration (~20% of 96 h-LC50) of 0.15 mg L−1 of mercury chloride (HgCl2) for 96 h in a static system. Increases in superoxide dismutase, catalase, glutathione peroxidase (GPx), glutathione S-transferase (GST) and glutathione reductase (GR) were observed in all tissues after HgCl2 exposure, except for white muscle GR activity and hepatic GPx. In the liver and gills, the exposure to HgCl2 also induced significant increases in reduced glutathione (GSH). Conversely, exposure to HgCl2 caused a significant decrease in the GSH levels and an increase in the oxidized glutathione (GSSG) content in the white muscle, while both GSH and GSSG levels increased significantly in the heart muscle. Metallothionein concentrations were significantly high after HgCl2 exposure in the liver, gills and heart, but remained at control values in the white muscle. HgCl2 exposure induced oxidative damage, increasing the lipid peroxidation and protein carbonyl content in all tissues. Mercury accumulated significantly in all the fish tissue. The pattern of accumulation follows the order gills > liver ≫ heart > white muscle. In conclusion, these data suggest that oxidative stress in response to inorganic mercury exposure could be the main pathway of toxicity induced by this metal in fish.

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Acknowledgments

This study was supported by São Paulo State Research Foundation (FAPESP—Proc. 06/50772-6) and the National Council for the Development of Research and Technology (CNPq). The authors are thankful to Águas Claras fish farm, which provided the fish.

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  1. Department of Physiological Sciences, Federal University of São Carlos, UFSCar, Via Washington Luís km 235, São Carlos, SP, Brazil

    Diana Amaral Monteiro, Francisco Tadeu Rantin & Ana Lúcia Kalinin

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  1. Diana Amaral Monteiro
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  3. Ana Lúcia Kalinin
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Correspondence to Ana Lúcia Kalinin.

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Monteiro, D.A., Rantin, F.T. & Kalinin, A.L. Inorganic mercury exposure: toxicological effects, oxidative stress biomarkers and bioaccumulation in the tropical freshwater fish matrinxã, Brycon amazonicus (Spix and Agassiz, 1829). Ecotoxicology 19, 105–123 (2010). https://doi.org/10.1007/s10646-009-0395-1

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