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Vanadium pentoxide effects on stress responses in wine Saccharomyces cerevisiae strain UE-ME3

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Abstract

Vanadium pentoxide mainly used as catalyst in sulphuric acid, maleic anhydride and ceramics industry, is a pollutant watering redistributed around the environment. Research on biological influence of vanadium pentoxide has gained major importance because it exerts toxic effects on a wide variety of biological systems. In this work we intent to evaluate the effects of vanadium pentoxide ranging from 0 to 2 mM in culture media on a wine wild-type Saccharomyces cerevisiae from Alentejo region of Portugal. Our results show that 2.0 mM vanadium pentoxide in culture medium induced a significant increase of malonaldehyde level and Glutathione peroxidase activity, a slightly increase of Catalase A activity as well as a decrease of wet weight and mitochondrial NADH cit c reductase of S. cerevisiae UE-ME3. Also our results show that cycloheximide prevent cell death when cells grows 30 min in presence of 1.5 mM of vanadium pentoxide.

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Abbreviations

V2O5 :

Vanadium pentoxide

MDA:

Malonaldehyde

CAT A:

Catalase peroxisomal

GPx:

Glutathione peroxidase

ROS:

Reactive oxygen species

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Acknowledgments

We acknowledge to “Instituto de Ciências Agrárias e Ambientais Mediterrânicas” (ICAM) at University of Évora, Portugal, by financial support of this work. We thanks to Engº Paulo Laureano of the Laboratory of Enology of University of Évora for the yielding of the yeast strain.

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Authors and Affiliations

  1. Departamento de Química, Universidade de Évora, R.Romão Ramalho, 59, 7002-671, Évora, Portugal

    Tânia Rosado, Ana Conim, Isabel Alves-Pereira & Rui Ferreira

  2. Instituto de Ciências Agrárias Mediterrânicas (ICAM) Colégio da Mitra, 7000-083, Évora, Portugal

    Isabel Alves-Pereira & Rui Ferreira

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  1. Tânia Rosado
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  2. Ana Conim
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  3. Isabel Alves-Pereira
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  4. Rui Ferreira
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Correspondence to Rui Ferreira.

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Rosado, T., Conim, A., Alves-Pereira, I. et al. Vanadium pentoxide effects on stress responses in wine Saccharomyces cerevisiae strain UE-ME3 . Ecotoxicology 18, 1116–1122 (2009). https://doi.org/10.1007/s10646-009-0363-9

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  • DOI: https://doi.org/10.1007/s10646-009-0363-9

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