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Antimicrobial activity of isolate HL-12 against Clavibacter michiganensis subsp. michiganensis in the presence of cadmium

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Abstract

An actinomycete, strain HL-12, that was isolated from a farmland on the Huajiachi campus of Zhejiang University was capable of inhibiting the growth of Clavibacter michiganensis subsp. michiganensis (Cmm) and was identified as a member of Streptomyces. Its antimicrobial activity against Cmm was measured using the agar plate sensitivity method in pure culture and evaluated by the inhibition ratio of Cmm in soil. The inhibitory activity of strain HL-12 against Cmm following exposure to low concentrations of Cd was greater than the inhibitory activity following exposure to high concentrations of Cd both in liquid culture and in soil. A stronger inhibition was also seen following a 24 h preculture in the presence of Cd in liquid culture. The growth of Cmm in soil was stimulated at low concentrations of Cd (<5.0 mg Cd kg−1 dry soil) but inhibited when cultured in high concentrations of Cd (5.0 and 10.0 mg Cd kg−1 dry soil). A higher inhibition ratio of strain HL-12 against Cmm, which was over 40% after soil incubation for 2 weeks, was observed following exposure to low concentrations of Cd (<5.0 mg Cd kg−1 dry soil).

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Acknowledgments

This work was financially supported by the 863 high technique program 2007AA10Z409 and the National Key Technologies Research and Development Program of China during the 11th Five-Year Plan Period (No. 2006BAJ08B01) and Science and Technology Bureau of Zhejiang Province (No. 2008C23088). Cmm was provided by Prof. Xie G.L.

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  1. Institute of Microbiology, College of Life Sciences, Zhejiang University, 388 Zijinhua Road, Hangzhou, Zhejiang, 310058, People’s Republic of China

    Hai-ping Yuan, Hang Min, Zhen-mei Lv & Zi-mu Li

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  1. Hai-ping Yuan
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  2. Hang Min
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  3. Zhen-mei Lv
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  4. Zi-mu Li
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Correspondence to Hang Min or Zhen-mei Lv.

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Yuan, Hp., Min, H., Lv, Zm. et al. Antimicrobial activity of isolate HL-12 against Clavibacter michiganensis subsp. michiganensis in the presence of cadmium. Ecotoxicology 18, 447–454 (2009). https://doi.org/10.1007/s10646-009-0299-0

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