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Phytohormone levels in germinating seeds of Zea mays L. exposed to selenium and aflatoxines

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Abstract

Seeds of Zea mays L. were exposed to aflatoxine B1 (AFB1), aflatoxine G1 (AFG1) and selenium (Se) alone and in combination and allowed to germinate. Phytohormone levels of GA-like substances (GAs), trans-Zeatin (t-Z) and Indole-3-acetic acid (IAA) were determined by High Performance Liquid Chromatography (HPLC) when the roots of the germinating seeds reach 1.5–3.0 cm in length. The levels of endogenous hormones decreased in seeds treated with AFB1 and AFG1 compared to control; however an increase was noted in seeds exposed to AFG1 and Se together. AFB1 and Se treatment caused reduced hormone levels in most of the treatments. When plants were exposed to Se alone, the highest levels of GAs, t-Z and IAA were observed in the application of 800 ppm Se. The highest levels of GAs, t-Z and IAA were observed when seeds were treated with 0.2 ppm AFG1 + 8 ppm Se, 0.2 ppm AFG1 + 8 ppm Se and 0.2 ppm AFG1 + 0.08 ppm Se, respectively, whereas the lowest levels of the hormones were observed in 0.2 ppm AFB1 + 8 ppm Se, 0.2 ppm AFB1 + 0.08 ppm Se and 0.1 ppm AFB1, respectively. In conclusion, the levels of phytohormones were reduced by the treatment of AFB1 and AFG1 alone. However Se removed the negative effect of AFB1 on phytohormones, but not AFB1.

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References

  • Abdou RF, Megella SE, Azab SG (1984) Mutogenic effect of aflatoxin B1 and G1 on the Egyptian cotton leaf-worm Spodoptera littoralis (Boisd). Mycopathologia 88:23–26

    Article  CAS  Google Scholar 

  • Ahmad SK (1993) Mycoflora changes and aflatoxin production in stored blackgram seeds. J Stored Prod Res 29:33–36

    Article  CAS  Google Scholar 

  • Anderson HW, Nehring EW, Wichser WR (1975) Aflatoxin contamination of corn in the field. J Agric Food Chem 23:775–782

    Article  CAS  Google Scholar 

  • Andresen I, Becker W, Schluter K, Burges J, Parthier B (1992) The identification of leaf thionin as one of the main jasmonate-induced proteins of barley (Hordeum Vulgare). Plant Mol Biol 19:193–204

    Article  CAS  Google Scholar 

  • Bathnagar D, Garcia S (2001) Aspergillus. In: Labbe RG, Garcia S (eds) Guide to foodborne pathogens. John Wiley and Sons, New York, pp 35–49

    Google Scholar 

  • Battal P, Aslan A, Turker M, Uzun Y (2004) The effect of gaseous air pollutant sulfur dioxide on phytohormone levels in some lichens. Fresinus Environ Bull 13(5):436–440

    CAS  Google Scholar 

  • Bell E, Mullet JE (1991) Lipoxygenase gene expression is modulated in plants by water deficit, wounding, and methyl jasmonate. Mol Gen Genet 230:456–462

    Article  CAS  Google Scholar 

  • Bilgrami KS, Sinha KK (1992) Aflatoxins their biological effects and ecological significance. In: Bhatnager D, Lillehoj EB, Arora DK (eds) Handbook of applied mycology, vol 5. Marcel Dekker, New York, pp 59–86

    Google Scholar 

  • Biswas S, Talukder G, Sharma A (1997) Selenium, salts and chromosome damage. Mutat Res 390:201–205

    CAS  Google Scholar 

  • Busley WF, Wogan GN (1979) Food-borne mycotoxins and alimeniary mycotoxicosis. In: Reiman HP, Bryan FL (eds) Food-borne infections and intoxications, 2nd edn. Academic Press, New York, pp 519–610

    Google Scholar 

  • Cakmak I, Marschner H, Bangert F (1989) Effect of zinc nutritional status on growth, protein metabolism and levels of indole-3-acetic acid and other phytohormones in bean (Phaseolus vulgaris L.). J Exp Bot 40:404–412

    Article  Google Scholar 

  • Chen J, Goetchius MP, Campbell TC, Combs GFJR (1982a) Effects of dietary selenium and vitamin E on hepatic mixed-function oxidase activities and in vivo covalent binding of aflatoxin B1 in rats. J Nutr 112:324–349

    CAS  Google Scholar 

  • Chen J, Goetchius MP, Combs GFJR, Campbell TC (1982b) Effects of dietary selenium and vitamin E on covalent binding of aflatoxin to chick liver cell macromolecules. J Nutr 112:350–355

    CAS  Google Scholar 

  • Chow CK, Gairola GC (1984) Influence of dietary vitamin E and selenium on metabolic activation of chemicals to mutagens. J Agric Food Chem 32:443–447

    Article  CAS  Google Scholar 

  • Combs GFJR (1997) Selenium and cancer. In: Garewal H (ed) Antioxidants and disease prevention. CRC Press, New York, pp 97–113

    Google Scholar 

  • Creelman RA, Tierney ML, Mullet JE (1992) Jasmonic acid/methyl jasmonate accumulate in wounded soybean hypocotyls and modulate wound gene expression. Proc Natl Acad Sci USA 89:4938–4941

    Article  CAS  Google Scholar 

  • Dashek WV, Llewellyn GC (1983) Mode of action of the hepatocarcinogens, aflatoxins in plant systems: a review. Mycopatologia 81:83–94

    Article  CAS  Google Scholar 

  • El-Bayoumy K (2001) The protective role of selenium on genetic damage and on cancer. Mutat Res 475:123–139

    CAS  Google Scholar 

  • El-Naghy MA, Fadl-Allah EM, Samhan M (1999) Effects of Aflotoxin G1 on germination, growth and metabolic activities of some crop plants. Cytobiosis 97:87–93

    CAS  Google Scholar 

  • Farmer EE, Ryan CA (1992) Octadecanoid precursors of jasmonic acid activate the synthesis of woundinducible proteinase inhibitors. Plant Cell 4:129–134

    Article  CAS  Google Scholar 

  • Ferguson LR, Philpott M (2004) Dietary cancer and prevention using antimutagens. Toxicology 198:147–159

    Article  CAS  Google Scholar 

  • Fujioka S, Sakurai A, Yamaguchi I, Murofiishi N, Takahashi N, Kaihari S, Takimota A, Cleland CF (1986) Flowering and Endogenous levels of plant hormones in Lemna species. Plant Cell Physiol 27:151–160

    Google Scholar 

  • Gerald F, Combs GFJR, William PG (1998) Chemopreventive agents: selenium. Pharmacol Ther 79:179–192

    Article  Google Scholar 

  • Gourama H, Bullerman LB (1995) Antimycotic and antiaflatoxigenic effect of lactic acid bacteria a review. J Food Protect 57:1275–1280

    Google Scholar 

  • Gqaleni N, Smith JE, Lacey J (1996) Co-production of aflatoxins and cyclopiazonic acid in isolates of Aspergillus flavus. Food Addit Contam 13:677–685

    CAS  Google Scholar 

  • Groopman JP, Croy RG, Wogan GN (1981) In vitro reactions of aflatoxin B1 aducted DNA. Proc Natl Acad Sci USA 78:5445–5449

    Article  CAS  Google Scholar 

  • Gundlach H, Muller MJ, Kutchan TM, Zenk MH (1992) Jasmonic acid is a signal transducer in elicitor-induced plant cell structures. Proc Natl Acad Sci USA 89:2389–2393

    Article  CAS  Google Scholar 

  • Hamilton SJ (1999) Hypothesis of historical effects from selenium on endangered fish in the Colorado River Basin. Hum Ecol Risk Assess 5:1153–1180

    Article  CAS  Google Scholar 

  • Hela K, Cardwell K, Setamou FM, Phoehlig HM (2000) The influence of storage practices on aflatoxin contamination in maize in four agroecological zones of Benin, west Africa. J Stored Prod Res 36:365–382

    Article  Google Scholar 

  • Hsieh DPH, Wong ZA, Wong JJ, Michas C, Ruebner BH (1977) Comparative metabolism of aflatoxin. In: Rodricks JV, Hesseltine CW, Mehlman MA (eds) Mycotoxins in human and animal health. Pathotox Publishers, Park Forest South, pp 37–50

    Google Scholar 

  • Hodson PV, Hilton JW (1983) The nutritional requirements and toxicity to fish of dietary and waterborne selenium. Ecol Bull (Stockholm) 35:335–340

    CAS  Google Scholar 

  • Kuraishi S, Tasaki K, Sakurai N, Sadatoku K (1991) Changes in levels of cytokinins in etiolated squash seedlings after illumination. Plant Cell Physiol 2:585–591

    Google Scholar 

  • Lemly AD (1985) Toxicology of selenium in a freshwater reservoir: implications for environmental hazard evaluations and safety. Ecotoxicol Environ Saf 10:314–338

    Article  CAS  Google Scholar 

  • Leontopoulos D, Siafaka A, Markaki P (2003) Black olives as substrate for Aspergillus parasiticus growth and aflatoxin B1 production. Food Microbiol 20:119–126

    Article  CAS  Google Scholar 

  • Lillehoj EB, Zuber MS (1988) Distribution of toxin-producing fungi in mature maize kernels from diverse environments. Trop Sci 28:19–24

    Google Scholar 

  • Lutz C (1994) The functioning of the maize market in Benin: spatial and temporal arbitrage on the market of a staple food crop. PhD Thesis, University of Amsterdam

  • Machackova L, Krekule J, Seidlova F, Strnad M (1993) Cytokinins in photoperiodic introduction of flowering Chenopodium species. Physiol Plant 87:160–166

    Article  CAS  Google Scholar 

  • McLean M, Watt MP, Berjak P, Dutton MF 1995. Aflatoxine B1-its effects on an in vitro plant system. Food Addit Contam 12(3):435–443

    CAS  Google Scholar 

  • Ong T (1971). Mutogenic activities of aflatoxins B1 and G1 in Neurospora crassa. Mol Gen Genet 111:159–160

    Article  CAS  Google Scholar 

  • Presser TS, Ohlendorf HM (1987) Biogeochemical cycling of selenium in the San Joaquin Valley. Environ Manage 11:805–821

    Article  CAS  Google Scholar 

  • Rayman MP (2000) The importance of selenium to human health (review). Lancet 356:233–241

    Article  CAS  Google Scholar 

  • Reis J (1978) Effects of mycotoxins on higher plants, algae, fungi and bacteria. In: Wyllie D, Morehouse LG (eds) Mycotoxic fungi, mycotoxins, mycotoxicoses: an encylopaedic handbook mycotoxicoses of man and plants: mycotoxin control and regulatory Practices. Marcel Dekker Inc, New York, pp 119–143

    Google Scholar 

  • Schrauzer GN, White DA, Schneider CJ (1977) Cancer mortality correlation studies-III: statistical associations with dietary selenium intakes. Bioinorg Chem 7:23–31

    Article  CAS  Google Scholar 

  • Sembdener G, Parthier B (1993) The biochemistry and the physiological and molecular actions of jasmonates. Annu Rev Plant Physiol 44:569–589

    Article  Google Scholar 

  • Shi CY, Chua SC, Lee HP, Ong CN (1994) Inhibition of aflatoxin B1-DNA binding and adduct formation by selenium in rats. Cancer Lett 82:203–208

    Article  CAS  Google Scholar 

  • Skorupa JP (1998) Selenium poisoning of fish and wildlife in nature: lessons from twelve real-world examples. In: Frankenberger W, Engberg RA (eds) Environmental chemistry of selenium. Marcel Dekker, New York, pp 315–354

    Google Scholar 

  • Spallholz JE (1994) On the nature of selenium toxicity and carcinostatic activity. Free Rad Biol Med 17:45–64

    Article  CAS  Google Scholar 

  • Wang Q, Little CHA, Sheng CO, Pharis RP (2002) Effect of exogenous gibberellin A4/7 on tracheid production, longitudinal growth and the levels of indole-3-acetic acid and gibberellins A4, A7 and A9 in terminal shoot of Pinus sylvestris seedlings. Physiol Plant 86:202–208

    Article  Google Scholar 

  • Wilbur COG (1983) Selenium – A potential environmental poison and a necessary food constituent. Springfield, IL, pp 134

  • Wogan GN (1992) Aflatoxins as risk factors for hepatocellular carcinoma in humans. Cancer Res 52:2114–2118

    Google Scholar 

  • Zeringue HJ (2002) Effects of methyl jasmonate on phytoalexin production and aflatoxin control in the developing cotton boll. Biochem System Ecol 30:497–503

    Article  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Faculty of Science-Art, Department of Biology, Ataturk University, Erzurum, Turkey

    Güleray Ağar

  2. Faculty of Science-Art, Department of Biology, Yuzuncu Yil University, Van, Turkey

    Musa Türker, Peyami Battal & M. Emre Erez

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  1. Güleray Ağar
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  2. Musa Türker
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  3. Peyami Battal
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  4. M. Emre Erez
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Correspondence to Peyami Battal.

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Ağar, G., Türker, M., Battal, P. et al. Phytohormone levels in germinating seeds of Zea mays L. exposed to selenium and aflatoxines. Ecotoxicology 15, 443–450 (2006). https://doi.org/10.1007/s10646-006-0079-z

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