Abstract
The V3-V4 region of the bacterial 16S rRNA in the intestinal contents of three commercial fish species (Larimichthys crocea, Larimichthys polyactis, and Trichiurus lepturus) from the central and southern East China Sea was sequenced in an attempt to understand the intestinal microbial diversity (IMD) and community structure in these populations collected in spring and autumn. Diversity analysis showed that the Shannon index, the Simpson index, the abundance-based coverage estimator (ACE) index, and the Chao1 index of the intestinal samples of the three commercial fish species were all lower in spring than in autumn, indicating that the richness of the intestinal microbiota of these three commercial fish species was lower. At the phylum level, Proteobacteria, Firmicutes, Actinobacteriota, and Bacteroidota were dominant among the intestinal microbiota of the three commercial fish species. Analysis of the composition and structure of the intestinal microbial community showed that the IMD and the community structure of the three commercial fish species were similar in a given season but differed significantly in different seasons. This study shows that the IMD and community structure of fish were significantly affected by season and feeding habits.
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Data availability
The raw data and associated codes for this project have now been uploaded to the NCBI public database. Biology project number: PRJNA913689.
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Acknowledgements
We would like to thank the Third Institute of Oceanography, Ministry of Natural Resources for providing the facilities required. This research was approved by the Animal Care Committee at Third Institute of Oceanography, Ministry of Natural Resources.
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The National Key Research and Development Program of China (2018YFC1406302).
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Jiali Xiang: literature search, data analysis, and writing—original draft. Shigang Liu: species identification and writing—original draft. Puqing Song and Ran Zhang: idea and supervision and species identification. Yuan Li: funding acquisition, material preparation, and data collection.: funding acquisition, the study conception, and design.
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Xiang, J., Liu, S., Song, P. et al. Differences in the diversity and community structure of the intestinal microbiota of commercial fishes in the central and southern East China Sea in spring and autumn. Environ Biol Fish 106, 1619–1629 (2023). https://doi.org/10.1007/s10641-023-01440-y
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DOI: https://doi.org/10.1007/s10641-023-01440-y