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Environmental Biology of Fishes

, Volume 100, Issue 5, pp 535–549 | Cite as

Thermal sensitivity of white muscle lactate dehydrogenase isolated from a lake trout, (Salmo trutta), inhabiting lake Plav, Montenegro

  • Miroslav Nikcevic
  • Branislav Mickovic
  • Zoran Gacic
  • Dragoslava Zivadinovic
Article

Abstract

To shed light on thermoadaptive properties of Salmo trutta from lake Plav (Montenegro), we undertook kinetic studies of pyruvate reduction rates and thermal stability analyses of white muscle LDH. We compared these with the data obtained for trout of the same, confirmed by us, Danubian lineage living in rivers and streams of Serbia and Montenegro. We also tested the effect of acclimation in captivity at 4 and 14 °C. The lake trout was of a typical smoltified phenotype (the size, the elongated silver colored body). At physiological substrate concentration, the breaks in the Arrhenius plots (critical temperature - Tc) correlated with acclimation temperatures or habitat water temperatures. Q10 values for temperatures above Tc were close to one, in all cases except 4 °C acclimated trout. At temperatures below Tc Q10 was close to two, except in the case of 14 °C acclimated trout. Lake trout had a highest Q10 values at temperatures below Tc. It was conspicuous that within the entire range of tested temperatures the differences in Q10 resulted from the effect of environmental temperature. Higher Q10 values were obtained with LDH isolated from trout acclimated to 4 °C compared with LDH acclimated to 14 °C. Ea values were much lower at a temperature below Tc compared with temperatures above Tc. Thermal stability of muscle LDH was lower after acclimation to 14 compared to 4 °C, while extremely high thermostability was obtained with the lake trout enzyme. Our data support the concept that Tc values have distinct physiological significance.

Keywords

Lake trout Temperature Smoltification Thermostability Muscle LDH 

Notes

Acknowledgements

We thank Dr. A. Apostolidis for his help with DNA sequencing and Dr. M. Paunović for providing the map with the geographic location of Lake Plav and the rivers where we sampled brown trout.

Compliance with Ethical Standards

Ethical approval

Treatment of animals was conducted in accordance with both national and international animal welfare standards and under supervison of IMSI’s Animal Care Comittee.

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Copyright information

© Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  1. 1.Institute for Multidisciplinary Research, Department of Natural Resources and Environmental SciencesUniversity of BelgradeBelgradeSerbia
  2. 2.Galveston CollegeGalvestonUSA

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