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Environmental Biology of Fishes

, Volume 98, Issue 7, pp 1823–1832 | Cite as

Life at the leading edge: genetic impoverishment of the spotted bass, Micropterus punctulatus, at its Western edge

  • J. W. Ray
  • M. Husemann
  • D. J. Lutz-Carrillo
  • R. S. King
  • P. D. Danley
Article

Abstract

Genetic variation was evaluated for spotted bass (Micropterus punctulatus) at the edge of the species native range in central Texas. We analyzed mitochondrial D-Loop sequences from 64 individuals representing nine sites within the Brazos and Trinity River watersheds. Our primary objectives were to 1) compare the genetic diversity of spotted bass in central Texas to genetic diversity estimates of conspecifics in other geographic regions and to other species from similar geographic regions, to 2) determine whether there was evidence for within or between drainage genetic structure, and to 3) test whether the D-Loop sequence was a reliable marker for discriminating among native and introduced Texas micropterids. Genetic diversity was low relative to sympatric micropterids, as well as for the marker in general, but our results are similar to patterns observed for this species in Oklahoma and fit with broader multi-species patterns observed for this geographic area. AMOVA, SAMOVA, and isolation by distance analyses provided evidence that genetic differentiation exists among population groups. The sequence data suggests the D-Loop would be useful for discriminating among M. floridanus, M. salmoides, and M. dolomieu, but not among M. punctulatus, M. coosae, M. henshalli, and M. treculii.

Keywords

Brazos River D-Loop Genetic diversity Molecular species identification 

Notes

Acknowledgments

We would like to thank J. Taylor, C. Stanley, J. Grimm, B. Kirchner, A. Flores, and E. Hooser for help with sampling specimens in hot Texas summers. We appreciate the constructive criticism and suggestions from the editor D. Noakes and two anonymous reviewers, which greatly improved the quality of the paper. Funding was provided by Texas Commission on Environmental Quality, Section 106 Water Pollution Control Grant # 98665304 to RSK, a Baylor University Summer Undergraduate Research Fellowship to Emily Hooser, and Baylor University funding to PDD and RSK. This project was performed under an approved Baylor University IACUC protocol and with TPWD scientific collection permits.

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • J. W. Ray
    • 1
    • 2
  • M. Husemann
    • 3
    • 4
  • D. J. Lutz-Carrillo
    • 5
  • R. S. King
    • 3
    • 6
  • P. D. Danley
    • 3
  1. 1.The Institute of Ecological, Earth and Environmental SciencesBaylor UniversityWacoUSA
  2. 2.U.S. Army Corps of EngineersLos AngelesUSA
  3. 3.Biology DepartmentBaylor UniversityWacoUSA
  4. 4.General Zoology, Institute of BiologyMartin-Luther University Halle-WittenbergHalle (Saale)Germany
  5. 5.Texas Parks and Wildlife DepartmentA.E. Wood LaboratorySan MarcosUSA
  6. 6.Center for Reservoir and Aquatic Systems ResearchBaylor UniversityWacoUSA

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