Skip to main content

Advertisement

Springer Nature Link
Log in

Life at the leading edge: genetic impoverishment of the spotted bass, Micropterus punctulatus, at its Western edge

  • Published:
Environmental Biology of Fishes Aims and scope Submit manuscript

Abstract

Genetic variation was evaluated for spotted bass (Micropterus punctulatus) at the edge of the species native range in central Texas. We analyzed mitochondrial D-Loop sequences from 64 individuals representing nine sites within the Brazos and Trinity River watersheds. Our primary objectives were to 1) compare the genetic diversity of spotted bass in central Texas to genetic diversity estimates of conspecifics in other geographic regions and to other species from similar geographic regions, to 2) determine whether there was evidence for within or between drainage genetic structure, and to 3) test whether the D-Loop sequence was a reliable marker for discriminating among native and introduced Texas micropterids. Genetic diversity was low relative to sympatric micropterids, as well as for the marker in general, but our results are similar to patterns observed for this species in Oklahoma and fit with broader multi-species patterns observed for this geographic area. AMOVA, SAMOVA, and isolation by distance analyses provided evidence that genetic differentiation exists among population groups. The sequence data suggests the D-Loop would be useful for discriminating among M. floridanus, M. salmoides, and M. dolomieu, but not among M. punctulatus, M. coosae, M. henshalli, and M. treculii.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+
from $39.99 /Month
  • Starting from 10 chapters or articles per month
  • Access and download chapters and articles from more than 300k books and 2,500 journals
  • Cancel anytime
View plans

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

Explore related subjects

Discover the latest articles, books and news in related subjects, suggested using machine learning.

References

  • Bagley JC, Mayden RL, Roe KJ, Holznagel W, Harris PM (2011) Congeneric phylogeographical sampling reveals polyphyly and novel biodiversity within black basses (Centrarchidae: Micropterus). Biol J Linn Soc 104:346–363

    Article  Google Scholar 

  • Bean PT, Lutz-Carrillo DJ, Bonner TH (2013) Rangewide survey of the introgressive status of Guadalupe bass: implications for conservation and management. T Am Fish Soc 142:681–689

    Article  Google Scholar 

  • Borden CW, Krebs RA (2009) Phylogeography and postglacial dispersal of smallmouth bass (Micropterus dolomieu) into the Great Lakes. Can J Fish Aquat Sci 66:2142–2156

    Article  Google Scholar 

  • Cleaveland MK, Votteler TH, Stahle DK, Casteel RC, Banner JL (2011) Extended chronology of drought in south central, southeastern and west Texas. Tex Water J 2:54–96

    Google Scholar 

  • Clement M, Posada D, Crandall K (2000) TCS: a computer program to estimate gene genealogies. Mol Ecol 9:1657–1660

    Article  CAS  PubMed  Google Scholar 

  • Coughlin WD, Echelle AA, van den Bussche RA, Cofer LM, Fisher WL (2003) Genetic structure of spotted bass (Micropterus punctulatus) in the Red and Arkansas River basins: microsatellite and mitochondrial DNA variation. Southwest Nat 48:526–533

    Article  Google Scholar 

  • Donaldson KA, Wilson RR Jr (1999) Amphia-panamic geminates of snook (Percoidei: Centropomidae) provide a calibration of the divergence rate in the mitochondrial DNA Control Region of fishes. Mol Phylogenet Evol 13:208–213

    Article  CAS  PubMed  Google Scholar 

  • Drummond AJ, Rambaut A, Shapiro B, Pybus OG (2005) Bayesian coalescent inference of past population dynamics from molecular sequences. Mol Biol Evol 22:1185–1192

    Article  CAS  PubMed  Google Scholar 

  • Drummond AJ, Ashton B, Buxton S, Cheung M, Cooper A, Duran C, Field M, Heled J, Kearse M, Markowitz S, Moir R, Stones-Havas S, Sturrock S, Thierer T, Wilson A, (2011) Geneious v5.4, Available at http://www.geneious.com/

  • Drummond AJ, Suchard MA, Xie D, Rambaut A (2012) Bayesian phylogenetics with BEAUti and the BEAST 1.7. Mol Biol Evol 29:1969–1973

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Dupanloup I, Schneider S, Excoffier L (2002) A simulated annealing approach to define the genetic structure of populations. Mol Ecol 11:2571–2581

    Article  CAS  PubMed  Google Scholar 

  • Excoffier L, Lischer HE (2010) Arlequin suite ver. 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Res 10:564–567

    Article  Google Scholar 

  • Hein J, Schierup MH, Wiuf C (2005) Gene genealogies, variation and evolution: a primer in coalescent theory. Oxford University Press, Oxford

    Google Scholar 

  • Huelsenbeck JP, Ronquist F (2001) MRBAYES: Bayesian inference of phylogeny. Bioinformatics 17:754–755

    Article  CAS  PubMed  Google Scholar 

  • Husemann M, Ray J, King RS, Hooser EA, Danley PD (2012) Comparative biogeography reveals differences in population genetic structure of five species of stream fish. Biol J Linn Soc 107:867–885

    Article  Google Scholar 

  • Jensen JL, Bohonak AJ, Kelley ST (2005) Isolation by distance, web service. BMC Genet 6:13

    Article  PubMed Central  PubMed  Google Scholar 

  • Kawamura K, Yonekura R, Katano O, Taniguchi Y, Saitoh K (2006) Origin and dispersal of bluegill sunfish, Lepomis macrochirus, in Japan and Korea. Mol Ecol 15:613–621

    Article  CAS  PubMed  Google Scholar 

  • Librado P, Rozas J (2009) DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25:1451–1452

    Article  CAS  PubMed  Google Scholar 

  • Littrell BM, Lutz-Carrillo DJ, Bonner TH, Fries LT (2007) Status of an introgressed Guadalupe bass populations in central Texas streams. N Am J Fish Manag 27:785–791

    Article  Google Scholar 

  • Lutz-Carrillo DJ, Nice CC, Bonner TH, Forstner RJ, Fries LT (2006) Admixture analysis of Florida largemouth bass and northern largemouth bass using microsatellite loci. T Am Fish Soc 135:779–791

    Article  Google Scholar 

  • Meyer A (1993) Evolution of mitochondrial DNA in fish. In Biochemistry and Molecular Biology of Fish (Hochachka and Mommsen eds.) pp 1–38. Elsevier Publishers, Amsterdam

  • Morizot DC, Calhoun SW, Clepper LL, Schmidt ME, Williamson JH, Carmichael GJ (1991) Multispecies hybridization among native and introduced centrarchid basses in central Texas. T Am Fish Soc 120:283–289

    Article  Google Scholar 

  • Mukai T, Sato C (2009) Complete mitochondrial DNA sequences of two haplotypes of the smallmouth bass, Micropterus dolomieu, collected from nonindigenous populations in Japan. Ichthyol Res 56:204–207

    Article  Google Scholar 

  • Near TJ, Kassler TW, Koppelman JB, Dillman CB, Philip DP (2003) Speciation in North American black basses, Micropterus (Actinopterygii: Centrarchidae). Evolution 57:1610–1621

    Article  PubMed  Google Scholar 

  • Near TJ, Bolnick DJ, Wainwright PC (2004) Investigating phylogenetic relationships of sunfishes and black basses (Actinopterygii: Centrarchidae) using DNA sequences from mitochondrial and nuclear genes. Mol Phylogenet Evol 32:344–357

    Article  CAS  PubMed  Google Scholar 

  • Near TJ, Bolnick DJ, Wainwright PC (2005) Fossil calibrations and molecular divergence time estimates in centrarchid fish (Teleostei: Centrarchidae). Evolution 59:1768–1782

    Article  PubMed  Google Scholar 

  • Pease A, Taylor JM, Winemiller KO, King RS (2011) Multiscale environmental influences on fish assemblage structure in Central Texas streams. T Am Fish Soc 140:1409–1427

    Article  Google Scholar 

  • Pierce PC, van den Avyle MJ (1997) Hybridization between introduced spotted bass and smallmouth bass in reservoirs. T Am Fish Soc 126:939–947

    Article  Google Scholar 

  • Posada D (2008) jModeltest: phylogenetic model averaging. Mol Biol Evol 25:1253–1256

    Article  CAS  PubMed  Google Scholar 

  • Rambaut A (2011) FigTree 1.3.1. Available at http://tree.bio.ed.ac.uk/software/figtree

  • Rambaut A, Drummond AJ (2009) Tracer v1.5. Available at http://beast.bio.ed.ac.uk/Tracer

  • Ray JW, Husemann M, Danley PD, King RS (2012) Genetic analysis reveals dispersal of Florida bass haplotypes from reservoirs to rivers in central Texas. T Am Fish Soc 141:1269–1273

    Article  Google Scholar 

  • Stanley CE, Taylor JM, King RS (2012) Coupling fish community structure with instream flow and habitat connectivity between two hydrologically extreme years. T Am Fish Soc 141:1000–1015

    Article  Google Scholar 

  • Templeton AR, Crandall KA, Sing CF (1992) A cladistic analysis of phenotypic associations with haplotypes inferred from restriction endonuclease mapping and DNA sequence data. III. Cladogram estimation. Genetics 132:619–633

    PubMed Central  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

We would like to thank J. Taylor, C. Stanley, J. Grimm, B. Kirchner, A. Flores, and E. Hooser for help with sampling specimens in hot Texas summers. We appreciate the constructive criticism and suggestions from the editor D. Noakes and two anonymous reviewers, which greatly improved the quality of the paper. Funding was provided by Texas Commission on Environmental Quality, Section 106 Water Pollution Control Grant # 98665304 to RSK, a Baylor University Summer Undergraduate Research Fellowship to Emily Hooser, and Baylor University funding to PDD and RSK. This project was performed under an approved Baylor University IACUC protocol and with TPWD scientific collection permits.

Author information

Author notes

  1. J. W. Ray

    Present address: U.S. Army Corps of Engineers, Los Angeles, CA, USA

  2. M. Husemann

    Present address: General Zoology, Institute of Biology, Martin-Luther University Halle-Wittenberg, Halle (Saale), 06120, Germany

Authors and Affiliations

  1. The Institute of Ecological, Earth and Environmental Sciences, Baylor University, Waco, TX, 76798, USA

    J. W. Ray

  2. Biology Department, Baylor University, Waco, TX, 76798, USA

    M. Husemann, R. S. King & P. D. Danley

  3. Texas Parks and Wildlife Department, A.E. Wood Laboratory, 505 Staples Road, San Marcos, TX, 78666, USA

    D. J. Lutz-Carrillo

  4. Center for Reservoir and Aquatic Systems Research, Baylor University, Waco, TX, 76798, USA

    R. S. King

Authors
  1. J. W. Ray
    View author publications

    Search author on:PubMed Google Scholar

  2. M. Husemann
    View author publications

    Search author on:PubMed Google Scholar

  3. D. J. Lutz-Carrillo
    View author publications

    Search author on:PubMed Google Scholar

  4. R. S. King
    View author publications

    Search author on:PubMed Google Scholar

  5. P. D. Danley
    View author publications

    Search author on:PubMed Google Scholar

Corresponding author

Correspondence to M. Husemann.

Additional information

J. W. Ray and M. Husemann contributed equally to this work.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ray, J.W., Husemann, M., Lutz-Carrillo, D.J. et al. Life at the leading edge: genetic impoverishment of the spotted bass, Micropterus punctulatus, at its Western edge. Environ Biol Fish 98, 1823–1832 (2015). https://doi.org/10.1007/s10641-015-0400-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10641-015-0400-x

Keywords