Abstract
Information regarding sexual maturity and reproductive cycles in skates has largely been based on gross morphological changes within the reproductive tract. While this information has proved valuable in obtaining life history information, it also necessitates sacrificing the skates to obtain this data. In contrast, few studies have used circulating steroid hormones to establish when these batoids become reproductively capable or for the determination of reproductive cyclicity. This study summarizes our current knowledge of hormonal analyses in determining skate reproductive status and offers information that suggests analysis of circulating steroid hormone concentrations provide a means to determine size at sexual maturity and asses reproductive cycles without the need to sacrifice the skate.
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References
Agresti A (2002) Categorical data analysis, 2nd edn. John Wiley and Sons, Inc, New York
Borg B (1994) Androgens in teleost fishes. Comp Biochem Physiol 109C:219–245
Callard G, Cuevas ME (1992) In vitro steroid secretion by staged spermatocysts (Sertoli/germ cell units) of dogfish (Squalus acanthias ) testis. Gen Comp Endocrinol 88:151–165
Cuevas ME, Callard GV (1989) In vitro secretion by Sertoli/ germ cell units (spermatocysts) derived from dogfish (Squalus acanthias) testis. Bull Mount Desert Island Biol Lab 28:30–31
Du Bois W, Mak P, Callard GV (1989) Sertoli cell function during spermatogenesis: the shark testis model. Fish Physiol Biochem 7:221–227
Ebert DA (2005) Reproductive biology of skates, Bathyraja (Ishiyama), along the eastern Bering Sea continental slope. J Fish Biol 66:618–649
Francis M, Maolagain CO, Stevens D (2001) Age, growth, and sexual maturity of two New Zealand endemic skates, Dipturus nasutus and D. innominatus. NZ J Mar Freshwater Res 35:831–842
Gelsleichter J, Rasmussen LEL, Manire CA, Tyminski J, Chang B, Lombardi-Carlson L (2002) Serum steroid concentrations and development of reproductive organs during puberty in male bonnethead sharks, Sphyrna tiburo. Fish Phys Biochem 26:389–401
Heupel MR, Whittier JM, Bennet MB (1999) Plasma steroid hormone profiles and reproductive biology of the epaulette shark, Hemiscyllium ocellatum. J Exp Zool 284:586–594
Kneebone J, Sulikowski JA, Ferguson DE, and Tsang PCW (2007) Endocrinological investigation into the reproductive cycles of two sympatric skate species, Malacoraja senta and Amblyraja radiata, in the western Gulf of Maine. Environ Bio Fish (This issue)
Koob TJ, Tsang P, Callard IP (1986) Plasma estradiol, testosterone and progesterone levels during the ovulatory cycle of the little skate, Raja erinacea. Biol Reprod 35:267–275
Koob TJ, Callard IP (1999) Reproductive endocrinology of female elasmobranchs: Lessons from the little skate (Raja erinacea) and spiny dogfish (Squalus acanthias). J Exp Zool 284:557–574
Manire CA, Rasmussen LEL, Hess DL, Hueter HE (1995) Serum steroid hormones and the reproductive cycle of the female bonnethead shark, Sphyrna tiburo. Gen Comp Endocrinol 97: 366–376
Manire CA, Rasmussen LEL, Gross TS (1999) Serum steroid hormones including 11-ketotestosterone, 11-ketoandrostenedione, and dihydroprogesterone in juvenile and adult bonnethead sharks, Sphyrna tiburo. J Exp Zool 284:595–603
Pratt HL (1988) Elasmobranch gonad structure—a description and survey. Copeia 1988:719–729
Rasmussen LEL, Murru FL (1992) Long term studies of serum concentrations of reproductively related steroid hormones in individual captive carcharhinids. Aus J Mar Freshwater Res 43:273–281
Rasmussen LEL, Hess DL, Luer CA (1999) Alterations in serum steroid concentrations in the clearnose skate, Raja eglanteria: correlations with season and reproductive status. J Exp Zool 284:575–585
Ruocco NL, Lucifora LO, Dı´az de Astarloa JM, Wöhler O (2006) Reproductive biology and abundance of the white-dotted skate, Bathyraja albomaculata, in the southwest Atlantic. ICES J Mar Sci 63:105–116
Snelson FF Jr., Rasmussen LEL, Johnson MR, Hess DL (1997) Serum concentrations of steroid hormones during reproduction in the Atlantic stingray, Dasyatis sabina. Gen Comp Endocrinol 108:67–79
Sulikowski JA, Tsang PCW, Howell WH (2004) Annual changes in steroid hormone concentrations and gonad development in the winter skate, Leucoraja ocellata. Mar Biol 144:845–853
Sulikowski JA, Tsang PCW, Howell WH (2005a) Age and size at sexual maturity for the winter skate, Leucoraja ocellata, in the western Gulf of Maine based on morphological, histological and steroid hormone analyses. Environ Biol Fish 72:429–441
Sulikowski JA, Kneebone J, Elzey S, Danley P, Howell WH, Tsang PCW (2005b) The reproductive cycle of the thorny skate, Amblyraja radiata, in the Gulf of Maine. Fish Bull 103:536–543
Sulikowski JA, Kneebone J, Elzey S, Howell WH, Tsang PCW. Tsang (2006) Using the composite parameters of reproductive morphology, histology and steroid hormones to determine age and size at sexual maturity for the thorny skate, Amblyraja radiata, in the western Gulf of Maine. J Fish Biol 69:1449–1465
Sumpter JP, Dodd JM (1979) The annual reproductive cycle of the female lessor spotted dogfish, Scyliorhinus canicula, and its endocrine control. J Fish Biol 15:687–695
Tricas TC, Maruska KP, Rasmussen LEL (2000) Annual cycles of steroid hormone production, gonad development, and reproductive behavior in the Atlantic stingray. Gene Comp Endocrinol 118:209–225
Tsang P, Callard IP (1982) Steroid production by isolated skate ovarian follicular cells. Bull Mt Desert Isl Biol Lab 22:96–97
Tsang P, Callard IP (1987) Morphological and endocrine correlates of the reproductive cycle of the aplacental viviparous dogfish, Squalus acanthias. Gen Comp Endocrinol 66:182–189
Tsang PCW, Callard IP (1992) Regulation of ovarian steroidogenesis in vitro in the viviparous shark, Squalus acanthias. J Exp Zool 261:97–104
Walmsley-Hart SA, Sauer WHH, Buxton CD (1999) The biology of the skates Raja wallacei and R. pullopunctata (Batoidea: Rajidae) on the Agulhas Bank, South Africa. South African J Mar Sci 21:165–179
Zeiner SJ, Wolf PG (1993) Growth characteristics and estimates of age at maturity of two species of skates (Raja binoculata and Raja rhina) from Monterey Bay, California. Conserv. Biol. Elasmobr., NOAA Technical Report NMFS. 115 pp
Acknowledgements
We thank Joe Jurek of the F/V Mystique Lady for all skate collections. Further thanks are extended to Scott Elzey and Suzie Biron (University of New Hampshire, Department of Zoology) for their help in processing of skates. This project was supported by a grant from the Northeast Consortium (NA16FL1324) to PCWT and JAS. This is contribution number #6 from University of New England’s Marine Science Center.
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Sulikowski, J.A., Driggers, W.B., Ingram, G.W. et al. Profiling plasma steroid hormones: a non-lethal approach for the study of skate reproductive biology and its potential use in conservation management. Environ Biol Fish 80, 285–292 (2007). https://doi.org/10.1007/s10641-007-9257-y
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DOI: https://doi.org/10.1007/s10641-007-9257-y