Skip to main content
SpringerLink
Log in

Population genetic diversity of Prenant’s schizothoracin, Schizothorax prenanti, inferred from the mitochondrial DNA control region

  • Brief Communication
  • Published:
Environmental Biology of Fishes Aims and scope Submit manuscript

Abstract

Wild populations of Prenant’s schizothoracin, Schizothorax prenanti, were collected from the Qingyi, Dadu, and Muli rivers in upper reaches of the Yangtze River. Genetic variation and population structure were examined based on analysis of the mitochondrial DNA control region. Nucleotide sequence analysis defined 30 haplotypes in 41 individuals. The nucleotide and haplotype diversities were very high, and genetic distances between the Dadu and Qingyi populations were much smaller than between either of them and the Muli population. The analysis of molecular variance demonstrated that most variation occurred within samples, and the differentiation between Muli and Dadu or Qingyi was significant. Estimates of gene flow indicated reproductive isolation between Muli and the other populations, and this divergence might be related to geographical location. The results are mostly consistent with the findings from AFLP analysis and also suggested considerable genetic diversity of the populations in the Muli, Dadu, and Qingyi rivers.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

References

  • Alvarado Bremer JR, Mejuto J, Greig TW, Ely B (1996) Global population structure of the swordfish (Xiphias gladius L.) as revealed by analysis of the mitochondrial DNA control region. J Exp Mar Biol Ecol 197:295–310

    Article  Google Scholar 

  • Brown JR, Beckenbach AT, Smith MJ (1993) Intraspecific DNA sequence variation of the mitochondrial control region of white sturgeon (Acipenser transmontanus). Mol Biol Evol 10:326–341

    PubMed  CAS  Google Scholar 

  • Ding R (ed) (1994) The fishes of Sichuan, China. Sichuan Publishing House of Science and Technology, Chengdu, Sichuan, China, p 641 (in Chinese)

    Google Scholar 

  • Excoffier L, Laval G, Schneider S (2005) Arlequin Ver. 3.0: an integrated software package for population genetics data analysis. Evol Bioinform Online 1:47–50

    CAS  Google Scholar 

  • Excoffier L, Smouse P, Quattro J (1992) Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131:479–491

    PubMed  CAS  Google Scholar 

  • Grunwald C, Stabile J, Waldman JR, Gross R, Wirgin I (2002) Population genetics of shortnose sturgeon Acipenser brevirostrum based on mitochondrial DNA control region sequences. Mol Ecol 11:1885–1898

    Article  PubMed  CAS  Google Scholar 

  • Guarniero I, Franzellitti S, Ungaro N, Tommasini S, Piccinetti C, Tinti F (2002) Control region haplotype variation in the central Mediterranean common sole indicates geographical isolation and population structuring in Italian stocks. J Fish Biol 60:1459–1474

    Article  Google Scholar 

  • Hudson RR, Slatkin M, Maddison WP (1992) Estimation of levels of gene flow from DNA sequence data. Genetics 132:583–589

    PubMed  CAS  Google Scholar 

  • Kimura M (1980) A simple method for estimating evolutionary rate of base substitution through comparative studies of nucleotide sequences. J Mol Evol 16:111–120

    Article  PubMed  CAS  Google Scholar 

  • Kumar S, Tamura K, Nei M (2004) MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163

    Article  PubMed  CAS  Google Scholar 

  • Liu H, Tzeng C-S, Teng H-Y (2002) Sequence variations in the mitochondrial DNA control region and their implications for the phylogeny of the Cypriniformes. Can J Zool 80:569–581

    Article  CAS  Google Scholar 

  • Meyer A (1993) Evolution of mitochondrial DNA in fish. In: Hochachka PW, Mommsen TP (eds) Biochemistry and molecular biology of fishes. Elsevier Science Publishers, Amsterdam, pp 1–38

    Google Scholar 

  • Moritz C, Dowling TE, Brown WM (1987) Evolution of animal mitochondrial DNA: relevance for population biology and systematics. Ann Rev Ecol Syst 18:269–292

    Article  Google Scholar 

  • Nei M (1987) Molecular evolutionary genetics. Columbia University Press, New York

    Google Scholar 

  • Nugroho E, Ferrell DJ, Smith P, Taniguchi N (2001) Genetic divergence of kingfish from Japan, Australia and New Zealand inferred by microsatellite DNA and mitochondrial DNA control region markers. Fish Sci 67:843–850

    Article  CAS  Google Scholar 

  • Nyakaana S, Arctander P, Siegismund HR (2002) Population structure of the African savannah elephant inferred from mitochondrial control region sequences and nuclear microsatellite loci. Heredity 89:90–98

    Article  PubMed  CAS  Google Scholar 

  • Rozas J, Sánchez-DelBarrio JC, Messeguer X, Rozas R (2003) DnaSP, DNA polymorphism analyses by the coalescent and other methods. Bioinformatics 19:2496–2497

    Article  PubMed  CAS  Google Scholar 

  • Sato S, Kojima H, Ando J, Ando H, Wilmot RL, Seeb LW, Efremov V, LeClair L, Buchholz W, Jin D-H, Urawa S, Kaeriyama M, Urano A, Abe S (2004) Genetic population structure of chum salmon in the Pacific Rim inferred from mitochondrial DNA sequence variation. Environ Biol Fish 69:37–50

    Article  Google Scholar 

  • Song J, Song Z, Yue B, Zheng W (2006) Assessing genetic diversity of wild populations of Prenant’s schizothoracin, Schizothorax prenanti, using AFLP markers. Environ Biol Fish 77:79–86

    Article  Google Scholar 

  • Stepien CA, Faber JE (1998) Population genetic structure, phylogeography and spawning philopatry in walleye (Stizostedion vitreum) from mitochondrial DNA control region sequences. Mol Ecol 7:1757–1769

    Article  PubMed  CAS  Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24:4876–4882

    Article  Google Scholar 

  • Weir BS, Cockerham CC (1984) Estimating F-statistics for the analysis of population structure. Evolution 38:1358–1370

    Article  Google Scholar 

  • Wilson AC, Cann RL, Carr SM, George M, Gyllensten UB, Helm-Bychowski KM, Higuchi RG, Palimbi SR, Prager EM, Sage RD, Stoneking M (1985) Mitochondrial DNA and two perspectives on evolutionary genetics. Biol J Linn Soc 26:375–400

    Article  Google Scholar 

Download references

Acknowledgments

This work was funded by the National Natural Science Foundation of China (No. 30670290) and Science and Technology Bureau of Sichuan Province, China (No. 03JY029-071-2). We would like to thank Taiming Yan, Chunlin He, and Tianming Xie for their help during sample collection. We are grateful to the anonymous reviewers for their helpful comments on the manuscript, and Josh Walters and David Punzalan for their revisions of English writing.

Author information

Authors and Affiliations

  1. Key Laboratory of Bio-resources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan University, Chengdu, 610064, China

    Zhaobin Song, Jun Song & Bisong Yue

Authors
  1. Zhaobin Song
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jun Song
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Bisong Yue
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Bisong Yue.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Song, Z., Song, J. & Yue, B. Population genetic diversity of Prenant’s schizothoracin, Schizothorax prenanti, inferred from the mitochondrial DNA control region. Environ Biol Fish 81, 247–252 (2008). https://doi.org/10.1007/s10641-007-9197-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10641-007-9197-6

Keywords

Search

Navigation