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The characteristics of multifocal electroretinogram in isolated perfused porcine eye

Cellular contributions to the in vitro porcine mfERG

Documenta Ophthalmologica volume 117, pages 205–214 (2008)Cite this article

Abstract

We investigated characteristics of multifocal electroretinograms (mfERG) from in vitro perfused porcine eyes. TTX, NMDA, APB, and PDA were used to identify contributions to the mfERG from inner retinal neurons, ON-pathway, OFF-pathway, and photoreceptors. The cellular contributions of the first-order kernel (K1) in an isolated perfused porcine mfERG came from both inner and outer retina, and were similar to those of in vivo porcine mfERG. In addition, application of isoflurane and propofol caused interference with the mfERG response which resembled inner retinal activities sensitive to TTX + NMDA application. Improved understanding of the cellular origins of the perfused porcine mfERG, in the absence of anesthetic agents, is useful for identifying changes shown in the waveform under anesthesia.

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Acknowledgments

The authors would like to thank Dr. Mohammad Shahidullah for helpful advice on perfusion set-up. This study was supported by the Competitive Earmark Research Grants (PolyU 5415/06M) from the Research Grants Committee of the Hong Kong SAR, and by the Niche Areas—Glaucoma Research (1-BB76) from The Hong Kong Polytechnic University.

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Affiliations

  1. Laboratory of Experimental Optometry (Neuroscience), School of Optometry, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong SAR, China

    Yiu-Fai Ng, Henry H. L. Chan, Chi-Ho To & Maurice K. H. Yap

Authors
  1. Yiu-Fai Ng
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  2. Henry H. L. Chan
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  3. Chi-Ho To
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  4. Maurice K. H. Yap
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Correspondence to Henry H. L. Chan.

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Ng, YF., Chan, H.H.L., To, CH. et al. The characteristics of multifocal electroretinogram in isolated perfused porcine eye. Doc Ophthalmol 117, 205–214 (2008). https://doi.org/10.1007/s10633-008-9124-y

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  • DOI: https://doi.org/10.1007/s10633-008-9124-y

Keywords

  • Multifocal electroretinography
  • Pig
  • Anesthesia
  • Retina