Abstract
Background
The complex mechanisms of colorectal cancer (CRC) pathogenesis and progression remain poorly understood. This study endeavors to unravel the role of UBXN11within the context of CRC.
Methods
UBXN11 expression level in CRC, stomach adenocarcinoma and esophageal carcinoma, and the overall survival in corresponding cancers were analyzed using UALCAN database. Human CRC cell lines and xenograft mouse model with UBXN11 overexpression were established to investigate the pathological role of UBXN11 in CRC progression. Luciferase assay, qPCR, and Western blot were performed to dissect the interaction between UBXN11 and NF-κB signaling.
Results
Heightened UBXN11 expression was observed in various digestive tract tumors, which was positively correlated with the reduced overall survival rates in CRC patients. Overexpression of UBXN11 significantly enhanced CRC cell proliferation in vitro and promoted tumor growth in vivo. Mechanistically, UBXN11 promoted CRC tumorigenesis through increasing the activation of NF-κB signaling pathway.
Conclusions
This study underscores the pivotal role of UBXN11 in CRC progression and paves the way for novel therapeutic strategies for CRC treatment.
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Data Availability
The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.
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Yubin Wang has given contributions to the conception of the design of the manuscript, analysis and interpretation of the data, and drafting the manuscript. Xiangbo Chen and Yubin Wang revised it critically. Xiangbo Chen, Changxin Chen, Rensong He, Yisen Huang, and Yubin Wang read and approved the final version of the manuscript.
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The study was approved by the Ethics Committee of Quanzhou First Hospital Affiliated to Fujian Medical University to ensure the adherence to ethical research standards. Written informed consent was also obtained from the participants.
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Chen, X., Chen, C., He, R. et al. UBXN11 Predicts as a Poor Index for Colorectal Cancer and Contributes to the Tumorigenesis by Activating NF-κB Signaling. Dig Dis Sci (2024). https://doi.org/10.1007/s10620-024-08414-9
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DOI: https://doi.org/10.1007/s10620-024-08414-9