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Inflammation of Mesenteric Adipose Tissue Correlates with Intestinal Injury and Disease Severity in Rats with Severe Acute Pancreatitis

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Abstract

Background

Visceral adipose tissue (VAT) is related to SAP prognosis. As a depot of VAT, mesenteric adipose tissue (MAT) resides between pancreas and gut, which might affect SAP and the secondary intestinal injury.

Aims

To investigate the changes of MAT in SAP.

Methods

24 SD rats were randomly divided into four groups. 18 rats in SAP group were euthanized in time gradients (6 h, 24 h, and 48 h after modeling) and the others in control group. Blood samples and tissues of pancreas, gut, and MAT were taken for analysis.

Results

Compared to the control group, SAP rats appeared MAT inflammation, presenting higher mRNA expression of TNF-α and IL-6 and lower IL-10, and histological changes after 6 h of modeling, which became worse over time. Flow cytometry showed that B lymphocytes increased in MAT after 24 h of SAP modeling and lasted up to 48 h, earlier than the changes of T lymphocytes and macrophages. The intestinal barrier integrity was damaged after 6 h of modeling, presenting lower mRNA and protein expression of ZO-1 and occludin, higher serum levels of LPS and DAO, with pathological changes, which gradually aggravated after 24 h and 48 h. SAP rats had higher serum levels of inflammatory indicators and revealed histological inflammation of pancreas, the severity of which increased with the passage of modeling time.

Conclusion

MAT appeared inflammation in early-stage SAP, and became worse over time, with the same trend as the intestinal barrier injury and the severity of pancreatitis. B lymphocytes infiltrated early in MAT, which might promote the MAT inflammation.

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Acknowledgments

We thank Zhe Wu for the assistance of research design and analysis. We thank Shizhong Yang for the clinical support.

Funding

This study was supported by the Youth Fund of Beijing Tsinghua Changgung Hospital (12022C1004) and Tsinghua Precision Medicine Foundation (10001020127).

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Authors and Affiliations

  1. Department of Gastroenterology, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, Beijing, 102218, China

    Qing Huang, Jiawen Liu, Zhiyong Zhou, Mingjun Zhang, Yutang Ren, Xuan Jiang & Bo Jiang

Authors
  1. Qing Huang
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  2. Jiawen Liu
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  3. Zhiyong Zhou
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  4. Mingjun Zhang
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  5. Yutang Ren
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Corresponding author

Correspondence to Xuan Jiang.

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All authors declare no competing interests.

Ethical approval

All protocols were approved by the Beijing Tsinghua Changgung Hospital Ethics Committee (22271-4-01).

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Huang, Q., Liu, J., Zhou, Z. et al. Inflammation of Mesenteric Adipose Tissue Correlates with Intestinal Injury and Disease Severity in Rats with Severe Acute Pancreatitis. Dig Dis Sci 68, 2474–2481 (2023). https://doi.org/10.1007/s10620-023-07846-z

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  • DOI: https://doi.org/10.1007/s10620-023-07846-z

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