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Estrogen Regulates Duodenal Calcium Absorption Through Differential Role of Estrogen Receptor on Calcium Transport Proteins

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Abstract

Background and Aims

Intestinal calcium absorption from the diet plays important role in maintaining calcium homeostasis in the body. Estrogen exerts wide physiological and pathological effects in the human. Previous studies have shown that estrogen is involved in the intestinal calcium absorption. In this study, we made investigation on the mechanism of estrogen action on duodenal calcium absorption.

Methods

The experiments were performed in mice, human, and human duodenal epithelial cells, SCBN cells. Murine duodenal calcium absorption was measured by using single pass perfusion of the duodenum in vivo. The calcium absorption of SCBN cells was evaluated by calcium imaging system. The expression of calcium transport proteins, transient receptor potential cation channel (TRPV6) and plasma membrane calcium pump (PMCA1b), in the duodenum or SCBN cells were analyzed by western blot.

Results

The duodenal calcium absorption in ovariectomized mice was significantly decreased, compared with control female mice, which returned to control level after 17β-estradiol replacement treatment. Estrogen regulated the expressions of TRPV6 and PMCA1b in murine and human duodenal mucosae and SCBN cells. The further results from SCBN cells showed that 17β-estradiol regulated calcium influx through the respective effects of estrogen receptor (ER) ɑ and β on TRPV6 and PMCA1b.

Conclusion

Estrogen regulates duodenal calcium absorption through differential role of ERɑ and ERβ on duodenal epithelial cellular TRPV6 and PMCA1b. The study further elucidates the mechanism of estrogen on the regulation of intestinal calcium absorption.

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Acknowledgments

This work was supported by National Natural Science Foundation of China to B. Tuo (31660285) and G. Wen (81460112).

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Author notes

  1. Xubiao Nie and Hai Jin have contributed equally to this work.

Authors and Affiliations

  1. Department of Gastroenterology, Affiliated Hospital, Zunyi Medical University, 149 Dalian Road, Zunyi, 563003, China

    Xubiao Nie, Hai Jin, Guorong Wen, Jingyu Xu, Jiaxing An, Xuemei Liu, Rui Xie & Biguang Tuo

  2. Digestive Disease Institute of Guizhou Province, Zunyi, China

    Xubiao Nie, Hai Jin, Guorong Wen, Jingyu Xu, Jiaxing An, Xuemei Liu, Rui Xie & Biguang Tuo

  3. Clinical Medical Research Center of Digestive Diseases of Guizhou Province, Zunyi, China

    Hai Jin, Guorong Wen, Jingyu Xu, Jiaxing An, Xuemei Liu, Rui Xie & Biguang Tuo

  4. Department of Gastroenterology and Hepatology, Hannover Medical School, Hannover, Germany

    Jingyu Xu

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  1. Xubiao Nie
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  2. Hai Jin
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Contributions

BT conceived and designed research; NX, HJ, GW, JX, JA, XL, and RX performed experiments; NX, HJ, and BT analyzed data; NX, HJ, GW, JX, JA, XL, RX, and BT interpreted results of experiments; NX, HJ, and BT prepared figures; NX drafted manuscript; BT edited and revised manuscript; NX, HJ, GW, JX, JA, XL, RX, and BT approved final version of manuscript.

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Correspondence to Biguang Tuo.

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Nie, X., Jin, H., Wen, G. et al. Estrogen Regulates Duodenal Calcium Absorption Through Differential Role of Estrogen Receptor on Calcium Transport Proteins. Dig Dis Sci 65, 3502–3513 (2020). https://doi.org/10.1007/s10620-020-06076-x

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  • DOI: https://doi.org/10.1007/s10620-020-06076-x

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