Differences Between the Intestinal Lumen Microbiota of Aberrant Crypt Foci (ACF)-Bearing and Non-bearing Rats

Abstract

Background

Multiple factors including host–microbiota interaction could contribute to the conversion of healthy mucosa to sporadic precancerous lesions. An imbalance of the gut microbiota may be a cause or consequence of this process.

Aim

The goal was to investigate and analyze the composition of gut microbiota during the genesis of precancerous lesions of colorectal cancer.

Methods

To analyze the composition of gut microbiota in the genesis of precancerous lesions, a rat model of 1, 2-dimethylhydrazine (DMH)-induced aberrant crypt foci (ACF) was established. The feces of these rats and healthy rats were collected for 16S rRNA sequencing.

Results

The diversity and density of the rat intestinal microbiota were significantly different between ACF-bearing and non-bearing group. ACF were induced in rats treated with DMH and showed increased expression of the inflammatory cytokines IL-6, IL-8, and TNF-α. Firmicutes was the most predominant phylum in both ACF-bearing and non-bearing group, followed by Bacteroidetes. Interestingly, although the density of Bacteroidetes decreased from the fifth week to the 17th week in both groups, it was significantly reduced in ACF-bearing group at the 13th week (P < 0.01). At the genus level, no significant difference was observed in the most predominant genus, Lactobacillus. Instead, Bacteroides and Prevotella were significantly less abundant (P < 0.01), while Akkermansia was significantly more abundant (P < 0.05) in ACF-bearing group at the 13th week.

Conclusion

Imbalance of the intestinal microbiota existed between ACF-bearing and non-bearing rats, which could be used as biomarker to predict the genesis of precancerous lesions in the gut.

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Funding

This Project is supported by the “Project of special Fund for science and technology of Sichuan” (Number: LY-55).

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Affiliations

Authors

Contributions

XLX and HAL conceived the project. TYH, TX, RPY, and YL performed the experiments. WBL and XLX analyzed the data and wrote/edited the manuscript.

Corresponding author

Correspondence to Hanan Long.

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Conflict of interest

Authors confirm that there is no conflict of interest.

Electronic supplementary material

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Supplemental Table 1

Operational taxonomic units (OTUs) produced bysequencing16S RNA genes of rats. NGS, non-bearing group stool; AGS, ACF-bearing group stool (XLSX 4108 kb)

Supplemental Table 2

Data of relative contributions of dominant phyla and genera in the intestinal lumen microbiota. NGS, non-bearing group stool; AGS, ACF-bearing group stool. “Others” represents the unclassified bacteria (XLSX 36 kb)

Supplemental Table 3

Relative abundance, median, and range of the predominant genera in the gut microbiota of ACF-bearing and non-bearing group.“Others” represents the unclassified bacteria (DOCX 24 kb)

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Xiao, X., Long, W., Huang, T. et al. Differences Between the Intestinal Lumen Microbiota of Aberrant Crypt Foci (ACF)-Bearing and Non-bearing Rats. Dig Dis Sci 63, 2923–2929 (2018). https://doi.org/10.1007/s10620-018-5180-7

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Keywords

  • Aberrant crypt foci (ACF)
  • Intestinal microbiota
  • Rat model
  • Precancerous lesions