Abstract
Background
miR-30a expression is down-regulated and regulates tumor suppressors in various cancers.
Aim
We investigated the mechanisms underlying the biological role of miR-30a in CRC.
Methods
MicroRNA, mRNA, and protein expression were analyzed by quantitative real-time PCR and Western blot. The migration and invasion abilities of CRC were determined by wound healing assay, and trans-well migration and invasion. A luciferase reporter assay was used to confirm the targets of miR-30a.
Results
miR-30a expression was significantly down-regulated in CRC tissues and in CRC tissue with lymph node metastasis compared to CRC tissue without metastasis. Overexpression of miR-30a suppressed migration and invasion through insulin-like growth factor 1 receptor (IGF1R) in CRC cells. miR-30a suppresses IGF1R protein expression and inhibits β-catenin or p-AKT and increases E-cadherin expression. The IGF1R expression level is also up-regulated in CRC tumors and inversely correlated with miR-30a in CRC specimens.
Conclusions
miR-30a functions as a tumor-suppressive miRNA, which may provide a therapeutic strategy for metastasis of CRC.
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Acknowledgments
This project is supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Science, ICT, and Future Planning (NRF-2015R1D1A3A01016026).
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YCL carried out the molecular genetic studies and drafted the manuscript. YRP participated in the sequenced alignment and drafted the manuscript. SLK helped to draft the Materials and methods, STL preformed the statistical analysis. SWK conceived of the study and coordination and helped to draft the manuscript. All authors read and approved the final manuscript.
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The human sample and clinical data used in this study were provided by the Biobank of Chonbuk National University Hospital, a member of the Korea Biobank Network, which is supported by the Ministry of Health, Welfare, and Family Affairs. This work was supported by Research Institute of Clinical Medicine of Chonbuk National University-Biomedical Research Institute of Chonbuk National University Hospital (IRB No; CUH-2016-04-018-002).
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Liu, Y.C., Park, Y.R., Kim, S.L. et al. MicroRNA-30a Inhibits Colorectal Cancer Metastasis Through Down-Regulation of Type I Insulin-Like Growth Factor Receptor. Dig Dis Sci 62, 3040–3049 (2017). https://doi.org/10.1007/s10620-017-4763-z
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DOI: https://doi.org/10.1007/s10620-017-4763-z