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Comparative Analysis of Mutational Profile of Sonic hedgehog Gene in Gallbladder Cancer

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Abstract

Background

Gallbladder cancer has high incidence in northeastern India; mortality too is high as the disease is often diagnosed late. Numerous studies have shown the role of sonic hedgehog (shh) in different cancers, an important ligand of the hedgehog signaling pathway.

Aim

This study was carried out to evaluate the shh gene mutations in gallbladder cancer patients.

Methods

PCR-SSCP was performed for shh gene in 50 samples each of gallbladder cancer, cholelithiasis, and control. The samples showing aberration in banding pattern were sequenced.

Results

Variation in banding pattern was observed in 20% gallbladder cancer cases, 10% in cholelithiasis, and none of the control (χ 2 = 11.111; p < 0.05). Sequencing results revealed seven novel point mutations in GBC cases. These novel mutations were found to be associated with histopathology (p < 0.05) and stage (p < 0.05) of gallbladder cancer.

Conclusion

This study reveals several novel individual and repetitive mutations of shh gene in GBC and cholelithiasis samples that may be used as diagnostic markers for gallbladder carcinogenesis.

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Authors and Affiliations

  1. Department of General Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221 005, India

    Ruhi Dixit & Vijay Kumar Shukla

  2. Department of Surgical Oncology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India

    Manoj Pandey

  3. Department of Forensic Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India

    Sunil Kumar Tripathi

  4. Department of Radio Diagnosis, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India

    Amit Nandan Dhar Dwivedi

Authors
  1. Ruhi Dixit
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  2. Manoj Pandey
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  3. Sunil Kumar Tripathi
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  4. Amit Nandan Dhar Dwivedi
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  5. Vijay Kumar Shukla
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Correspondence to Vijay Kumar Shukla.

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All the authors declared that they have no conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional research committee.

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Informed consent was obtained from all individual participants included in the study.

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Dixit, R., Pandey, M., Tripathi, S.K. et al. Comparative Analysis of Mutational Profile of Sonic hedgehog Gene in Gallbladder Cancer. Dig Dis Sci 62, 708–714 (2017). https://doi.org/10.1007/s10620-016-4438-1

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  • DOI: https://doi.org/10.1007/s10620-016-4438-1

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