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High Expression of BCCIP β Can Promote Proliferation of Esophageal Squamous Cell Carcinoma

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Abstract

Background

BCCIP was originally identified as a BRCA2 interacting protein in humans and Ustilago maydis. It had low expression in some human cancer tissues. However, recent research indicated that many caretaker genes are also necessary for cell viability and their expression could contribute to tumor progression.

Aim

To characterize whether BCCIP is a caretaker gene in esophageal squamous cell carcinoma (ESCC).

Methods

Western blotting and immunohistochemistry were used to measure the expression of BCCIP β. In vitro studies were used to verify the effects of BCCIP β in Eca109 cells.

Results

Expression of BCCIP β was notably higher in tumor tissues of ESCC and Eca 109 cells. Meanwhile, the immunohistochemistry stain revealed that BCCIP β was positively correlated with clinical pathologic variables such as tumor size and tumor grade, as well as Ki-67, and prompted poor prognosis. In vitro studies such as starvation and refeeding assay along with BCCIP β-shRNA transfection assay demonstrated that BCCIP β expression promoted proliferation of ESCC cells. In addition, BCCIP β downregulation by silencing RNA significantly decreased the rate of colony formation, alleviated cellular apoptosis and increased the chemosensitivity of cisplatin.

Conclusions

This research first put forward that BCCIP β is an oncogene in human ESCC and contributes to the poor outcome of the deadly disease.

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Acknowledgements

This work was supported by Grants from the National Natural Science Foundation of China (Nos. 81171140, 81472272).

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Author notes

    Authors and Affiliations

    1. Department of Gastroenterology, Affiliated Hospital of Nantong University, 19 Qixiu Road, Nantong, 226001, Jiangsu, China

      Lingling Chen, Zhipeng Lin & Runzhou Ni

    2. Department of Medical Oncology, Affiliated Hospital of Nantong University, 19 Qixiu Road, Nantong, 226001, Jiangsu, China

      Sujie Ni, Mei Li, Chaoyan Shen, Li Liang & Wenyan Jiang

    3. Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong, 226001, Jiangsu, China

      Yu Ouyang

    4. Department of Radiology, Affiliated Hospital of Nantong University, 19 Qixiu Road, Nantong, 226001, Jiangsu, China

      Fei Xia

    5. Department of Pathology, Affiliated Hospital of Nantong University, 19 Qixiu Road, Nantong, 226001, Jiangsu, China

      Jianguo Zhang

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    1. Lingling Chen
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    Correspondence to Runzhou Ni or Jianguo Zhang.

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    Lingling Chen and Sujie Ni have contributed equally to this work.

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    Fig. S1

    BCCIP expression in ESCC tissues. Seven paired samples of ESCC tumor tissues (T) and adjacent non-tumor tissues (N). This figure with the primary data suggests that BCCIP α expression is not as evident as in BCCIP β and is even silenced in tumor tissues (TIFF 1157 kb)

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    Chen, L., Ni, S., Li, M. et al. High Expression of BCCIP β Can Promote Proliferation of Esophageal Squamous Cell Carcinoma. Dig Dis Sci 62, 387–395 (2017). https://doi.org/10.1007/s10620-016-4382-0

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    • DOI: https://doi.org/10.1007/s10620-016-4382-0

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