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Follow-Up Study on CDX1 and CDX2 mRNA Expression in Noncancerous Gastric Mucosae After Helicobacter pylori Eradication

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Abstract

Background

Changes in CDX1/CDX2 in gastric mucosae following Helicobacter pylori eradication have not been clarified yet.

Aims

To evaluate the changes in CDX1/CDX2 expression after H. pylori eradication, in relation to the reversibility of intestinal metaplasia (IM).

Methods

Time course of CDX1/CDX2 expressions was investigated in 176 subjects with various gastroduodenal disorders. Among them, 132 patients were H. pylori positives; H. pylori were eradicated in 107 of them; 13 failed to eradicate; and 12 did not receive H. pylori eradication therapy. Forty-four subjects were H. pylori negatives. Expression levels in CDX1 and CDX2 from noncancerous gastric mucosae of the corpus, as well as the histologic findings of gastric mucosae, were evaluated during the follow-up.

Results

Average follow-up duration was 33.7 months (range 2–97 months). Expression levels in both CDX1 and CDX2 mRNAs were correlated with IM grade in the corpus (ρ = 0.633 and 0.554, respectively, all P < 0.001). Changes in CDX1/CDX2 mRNA expressions following H. pylori eradication showed only insignificant results; IM grade at the antrum and corpus showed a tendency to decrease after H. pylori eradication without statistical significance (P > 0.05). However, histologic improvement of IM at the corpus was associated with a decrease in CDX2 mRNA expression during the follow-up (linear mixed model, P for slope = 0.015).

Conclusions

In this study, eradication of H. pylori did not show any beneficial effects on aberrant CDX1/CDX2 expressions or IM. Reversibility of IM may be associated with a decrease in CDX2 mRNA expression.

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Acknowledgments

This work was supported by the National Research Foundation of Korea (NRF) grant for the Global Core Research Center (GCRC) funded by the Korea government (MSIP, No. 2011-0030001), and by the Seoul National University Bundang Hospital Research Fund (Grant No. 02-2011-051).

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Authors and Affiliations

  1. Department of Internal Medicine, Seoul National University Bundang Hospital, 173-82, Gumi-ro, Bundang-gu, Seongnam, Gyeonggi-do, 463-707, South Korea

    Cheol Min Shin, Nayoung Kim, Hyun Chang & Dong Ho Lee

  2. Department of Internal Medicine and Liver Research Institute, Seoul National University College of Medicine, Seoul, South Korea

    Nayoung Kim, Joo Sung Kim, Dong Ho Lee & Hyun Chae Jung

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  1. Cheol Min Shin
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  2. Nayoung Kim
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Correspondence to Nayoung Kim.

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Supplementary material 1 (TIFF 658 kb)

10620_2016_4048_MOESM2_ESM.tif

Supplementary Figure S2. Changes in CDX1 mRNA expression levels during the follow-up according to the improvement or aggravation of intestinal metaplasia (IM) grade at the corpus (n = 176). Histologic improvement of IM was associated with a decrease in CDX1 mRNA expression levels (A) and aggravation of IM with an increase of CDX1 mRNA levels (C), which was not statistically significant (P values for slope > 0.05) (TIFF 6627 kb)

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Shin, C.M., Kim, N., Chang, H. et al. Follow-Up Study on CDX1 and CDX2 mRNA Expression in Noncancerous Gastric Mucosae After Helicobacter pylori Eradication. Dig Dis Sci 61, 1051–1059 (2016). https://doi.org/10.1007/s10620-016-4048-y

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  • DOI: https://doi.org/10.1007/s10620-016-4048-y

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