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Melatonin Attenuates Dextran Sodium Sulfate Induced Colitis with Sleep Deprivation: Possible Mechanism by Microarray Analysis

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Abstract

Background

Inflammatory bowel disease is a chronic inflammatory condition of the gastrointestinal tract. It can be aggravated by stress, like sleep deprivation, and improved by anti-inflammatory agents, like melatonin. We aimed to investigate the effects of sleep deprivation and melatonin on inflammation. We also investigated genes regulated by sleep deprivation and melatonin.

Methods

In the 2 % DSS induced colitis mice model, sleep deprivation was induced using modified multiple platform water bath. Melatonin was injected after induction of colitis and colitis with sleep deprivation. Also mRNA was isolated from the colon of mice and analyzed via microarray and real-time PCR.

Results

Sleep deprivation induced reduction of body weight, and it was difficult for half of the mice to survive. Sleep deprivation aggravated, and melatonin attenuated the severity of colitis. In microarrays and real-time PCR of mice colon tissues, mRNA of adiponectin and aquaporin 8 were downregulated by sleep deprivation and upregulated by melatonin. However, mRNA of E2F transcription factor (E2F2) and histocompatibility class II antigen A, beta 1 (H2-Ab1) were upregulated by sleep deprivation and downregulated by melatonin.

Conclusion

Melatonin improves and sleep deprivation aggravates inflammation of colitis in mice. Adiponectin, aquaporin 8, E2F2 and H2-Ab1 may be involved in the inflammatory change aggravated by sleep deprivation and attenuated by melatonin.

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Acknowledgments

We thank E biogen for help in analyzing micro DNA data.

Conflict of interest

This research was supported by EMBRI Grants 2010-SN-05 from the Eulji University.

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Authors and Affiliations

  1. Department of Gastroenterology, Ajou University School of Medicine, Suwon, Korea

    Sook Hee Chung

  2. Department of Internal Medicine, Eulji Hospital, Eulji University School of Medicine, 280-1 H«agye 1-dong, Nowon-gu, Seoul, 139-711, Korea

    Young Sook Park, Yunju Jo, Sang Bong Ahn, Young Kwan Jo, Byoung Kwan Son & Seong Hwan Kim

  3. Department of Biochemistry, Eulji University School of Medicine, Seoul, Korea

    Ok Soon Kim, Ja Hyun Kim & Haing Woon Baik

  4. Department of Pathology, Eulji University School of Medicine, Seoul, Korea

    Young Ok Hong

  5. Eulji Bio-Medical Research Institute, Eulji University School of Medicine, Seoul, Korea

    Sang Su Kim

  6. Eulji University School of Medicine, Seoul, Korea

    Jae-Ho Shin & Jin-Hyun Jun

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  1. Sook Hee Chung
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Correspondence to Young Sook Park.

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Chung, S.H., Park, Y.S., Kim, O.S. et al. Melatonin Attenuates Dextran Sodium Sulfate Induced Colitis with Sleep Deprivation: Possible Mechanism by Microarray Analysis. Dig Dis Sci 59, 1134–1141 (2014). https://doi.org/10.1007/s10620-013-3013-2

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