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Ischemia/Reperfusion in Clamped Lobes Facilitates Liver Regeneration of Non-clamped Lobes After Selective Portal Vein Ligation

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Abstract

Background

Hypertrophy of non-clamped liver lobes and the atrophy of clamped lobes have been shown to be interactive. Here, a rat model of selective lobe occlusion was established to study the effect of contralateral ischemia/reperfusion (I/R) on regeneration of non-clamped lobes.

Methods

Left lateral and middle liver lobes were pretreated with I/R. In the experimental (IR + PVL) group, portal veins of the left and middle lobes were ligated. A group given similar portal vein ligation but no I/R (PVL) was the positive control.

Results

Compared with the PVL group, the IR + PVL had higher, but not remarkable, levels of serum transaminases; weights of non-clamped lobes in the IR + PVL group comparatively increased much more notably. At 24-h post-surgery, the IR + PVL group’s PCNA mRNA was up-regulated compared with the PVL group. At 72-h post-surgery, PCNA protein was up-regulated significantly, while TGF-β1 was down-regulated in the IR + PVL group notably, compared with the PVL group.

Conclusion

The I/R pretreatment given to the clamped lobes facilitates liver regeneration of non-clamped lobes after selective portal vein ligation, which may result from down-regulated TGF-β1 expression in non-clamped lobes.

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Acknowledgments

This study was supported by a Research Fund for the Doctor of Harbin Medical University (no. BS 2007-15), Health Department of Heilongjiang Province (2010-075) and National Natural Science Foundation of China (No. 81170426).

Conflict of interest

None.

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Authors and Affiliations

  1. Department of Hepatopancreatobiliary Surgery, Second Affiliated Hospital of Harbin Medical University, 157 Baojian Road, Harbin, 150086, China

    Jian-hua Yu, Jia-yun Zhao, Zhi-dong Wang & Yun-fu Cui

  2. Department of General Surgery, Shaoxing People’s Hospital, Shaoxing, 312000, China

    Jian-hua Yu

  3. Department of Biochemistry and Molecular Biology, Basic Medical Science College, Harbin Medical University, Harbin, 150081, China

    Wei-guang Zhang & Hui Li

  4. Department of General Surgery, Second Affiliated Hospital of Zhejiang University, Hangzhou, 310003, China

    Gui-xing Jiang

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  1. Jian-hua Yu
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  2. Wei-guang Zhang
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  3. Gui-xing Jiang
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  4. Jia-yun Zhao
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  5. Hui Li
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  6. Zhi-dong Wang
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  7. Yun-fu Cui
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Correspondence to Zhi-dong Wang or Yun-fu Cui.

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Yu, Jh., Zhang, Wg., Jiang, Gx. et al. Ischemia/Reperfusion in Clamped Lobes Facilitates Liver Regeneration of Non-clamped Lobes After Selective Portal Vein Ligation. Dig Dis Sci 57, 3178–3183 (2012). https://doi.org/10.1007/s10620-012-2298-x

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  • DOI: https://doi.org/10.1007/s10620-012-2298-x

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