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Protective Effects of 3,4-Oxo-isopropylidene-Shikimic Acid on Experimental Colitis Induced by Trinitrobenzenesulfonic Acid in Rats

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Abstract

Background

3,4-Oxo-isopropylidene-shikimic acid (ISA) is a derivative of shikimic acid (SA). SA is extracted from Illicium verum Hook.fil., which has been used in traditional Chinese medicine and used for treating vomiting, stomach aches, insomnia, skin inflammation, and rheumatic pain.

Aims

To investigate the effects and the protective mechanism of 3,4-oxo-isopropylidene-shikimic acid on experimental colitis model induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS) in rats.

Methods

Colitis in rats was induced by colonic administration with TNBS. ISA (50, 100, and 200 mg/kg) was administered for 12 days to experimental colitis rats. The inflammatory degree was assessed by macroscopic damage score, colon weight/length ratios (mg/cm), and myeloperoxidase (MPO) activity. Malondialdehyde (MDA), glutathione (GSH), and nitric oxide (NO) levels, and superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), inducible nitric oxide synthase (iNOS) activities were measured with biochemical methods.

Results

ISA significantly ameliorated macroscopic damage, reduced colon weight/length ratios and the activity of MPO, depressed MDA and NO levels and iNOS activity, and enhanced GSH level, and GSH-Px and SOD activities in the colon tissues of experimental colitis in a dose-dependent manner. Moreover, the effect of ISA (200 mg/kg) was as effective as sulfasalazine (500 mg/kg).

Conclusions

The findings of this study demonstrate the protective effect of ISA on experimental colitis, probably due to an antioxidant action.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No 30973578 and No 30973673).

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Authors and Affiliations

  1. Department of Pharmacy, College of Medicine, Xi’an Jiaotong University, Xi’an, 710061, China

    Jian-Feng Xing, Cui-Yu You & Kai Dong

  2. Beijing University of Traditional Chinese Medicine, Beijing, 100102, China

    Jian-Ning Sun

  3. Department of Pharmacy, The First Affiliated Hospital of Medical College, Xi’an Jiaotong University, Xi’an, 710061, China

    Jin-Yao Sun, Jun Lv & Ya-Lin Dong

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  1. Jian-Feng Xing
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Correspondence to Ya-Lin Dong.

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Xing, JF., Sun, JN., Sun, JY. et al. Protective Effects of 3,4-Oxo-isopropylidene-Shikimic Acid on Experimental Colitis Induced by Trinitrobenzenesulfonic Acid in Rats. Dig Dis Sci 57, 2045–2054 (2012). https://doi.org/10.1007/s10620-012-2155-y

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