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Effects of RNAi-Mediated Matrix Metalloproteinase-2 Gene Silencing on the Invasiveness and Adhesion of Esophageal Carcinoma Cells, KYSE150

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Abstract

Background

Esophageal carcinoma is one of the main malignancies in China. Previous studies indicated that matrix metalloproteinases (MMPs) play important roles in the process of tumor invasion and metastasis in several types of solid tumors. Among all of the MMPs, MMP-2 is one of the MMPs closely associated with tumor invasion. In this study, we suppressed MMP-2 expression with RNA interference and then observed inhibitory effects on the invasion and migration of the esophageal carcinoma cell line KYSE150.

Methods

Three target sequences were selected and siRNA against MMP-2 mRNA were synthesized. After being transfected by the transfection complexes, the MMP-2 expression of KYSE150 cells, which overexpresses MMP-2, were examined by Western blot analysis and real-time polymerase chain reaction (PCR). Cell migration and invasion were measured with migration assay and Boyden chamber assays, respectively.

Results

RNAi against MMP-2 successfully inhibited the mRNA and protein expression of MMP-2 in the esophageal carcinoma cell line KYSE150. MMP-2 knockdown inhibited the invasion and migration of esophageal carcinoma cell line KYSE150.

Conclusions

These findings suggested that the RNAi approach towards MMP-2 may be a potentially effective therapeutic method for the treatment of esophageal carcinoma.

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Acknowledgments

The authors thank Chunting Zhang and Hailin Ren (the second hospital of Tianjin Medical University, China) for their helpful discussion of the manuscript and Shujun Li of the Tianjin Lung Cancer Research Institute for the technical assistance.

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Authors and Affiliations

  1. Thoracic Department, Tianjin Chest Hospital, Tianjin Medical University, Xi’an Road, Tianjin, 300051, People’s Republic of China

    Yu-Guang Shen, Yi-Jun Xu, Zhen-Liang Shi, Hong-Li Han, Da-Qiang Sun & Xun Zhang

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  1. Yu-Guang Shen
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  2. Yi-Jun Xu
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  3. Zhen-Liang Shi
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  4. Hong-Li Han
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  5. Da-Qiang Sun
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  6. Xun Zhang
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Corresponding author

Correspondence to Xun Zhang.

Additional information

Yu-Guang Shen and Yi-Jun Xu contributed equally to this work.

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Shen, YG., Xu, YJ., Shi, ZL. et al. Effects of RNAi-Mediated Matrix Metalloproteinase-2 Gene Silencing on the Invasiveness and Adhesion of Esophageal Carcinoma Cells, KYSE150. Dig Dis Sci 57, 32–37 (2012). https://doi.org/10.1007/s10620-011-1864-y

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  • DOI: https://doi.org/10.1007/s10620-011-1864-y

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