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Effects of Macrophage Metalloelastase on the Basic Fibroblast Growth Factor Expression and Tumor Angiogenesis in Murine Colon Cancer

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Abstract

Background and Aim

Previous studies have shown that overexpression of macrophage metalloelastase (MME) suppresses tumor growth in mice. The purpose of this study was to investigate the effects of MME on basic fibroblast growth factor (bFGF) expression and tumor angiogenesis in murine colon cancer.

Methods

Murine CT-26 colon cancer cells stably transfected with MME were inoculated subcutaneously. Reverse-transcriptase polymerase chain reaction (RT-PCR), immunoblotting, and immunohistochemistry were used to explore the bFGF mRNA and protein expression. Immunohistochemical staining of CD34 was used to measure the microvessel density (MVD).

Results

bFGF mRNA levels in tumor tissues of CT-26-EGFP and nontransfected cells were respectively 2.7-fold (0.56 ± 0.063 vs. 0.21 ± 0.042) and 2.5-fold (0.53 ± 0.066 vs. 0.21 ± 0.042) higher than that in tumors of CT-26-EGFP-MME cells (p < 0.01). bFGF protein levels exhibited a similar trend. Tumors of CT-26-EGFP-MME cells demonstrated a lower microvessel density (9.35 ± 2.79) than control tumors of CT-26-EGFP cells (22.85 ± 3.80) and nontransfected cells (23.45 ± 4.49) (p < 0.001).

Conclusions

We found that expression of MME inversely correlates with the expression of bFGF and tumor angiogenesis in a model of murine colon cancer. These data indicate that manipulation of MME expression could be a novel modality approach to colon cancer therapy.

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Acknowledgments

This work was supported by the Natural Science and Technology Foundation of Anhui Province (01043904) and the Natural Science Foundation of Education Department of Anhui Province (2003 KG199). We sincerely thank The Key Laboratory of Gene Resource Utilization for Genetic Ministry of Education and Anhui Province and especially M. Haiping Wang, Qi Li, and Lijie Feng.

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Authors and Affiliations

  1. Department of Gastroenterology, the First Affiliated Hospital, Anhui Medical University, Hefei, 230022, Anhui Province, People’s Republic of China

    Zhangwei Xu, Hai Shi, Qiao Mei & Jianming Xu

  2. The Key Laboratory of Gastroenterology of Anhui Province, Hefei, People’s Republic of China

    Zhangwei Xu & Jianming Xu

  3. The Key Laboratory of Gene Resource Utilization for Genetic Diseases, Ministry of Education and Anhui Province, Hefei, 230022, Anhui Province, People’s Republic of China

    Yuxian Shen

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  1. Zhangwei Xu
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Correspondence to Jianming Xu.

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Xu, Z., Shi, H., Mei, Q. et al. Effects of Macrophage Metalloelastase on the Basic Fibroblast Growth Factor Expression and Tumor Angiogenesis in Murine Colon Cancer. Dig Dis Sci 57, 85–91 (2012). https://doi.org/10.1007/s10620-011-1838-0

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  • DOI: https://doi.org/10.1007/s10620-011-1838-0

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