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NaOH-Induced Crohn’s Colitis in Rats: A Novel Experimental Model

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Abstract

Aim

Several models of experimental ulcerative colitis have been previously reported. To date, only one model of Crohn’s colitis that was induced by trinitrobenzene sulfonic acid has been described.

Materials and Methods

Twenty-one male albino rats were divided into three groups: group I: sham control group (n = 7), group II: acetic acid group (n = 7), group III: NaOH group (n = 7). On the day of induction, all rats were lightly anesthetized with intramuscular ketamine (8 mg/kg). A 6F plastic catheter was inserted rectally until the tip was 5 cm proximal to the anus. Then, 2 ml of 0.9% saline, 2 ml of 4% acetic acid, and 2 ml of 6.25% NaOH was administered to groups I, II, and III, respectively. All rats were sacrificed 5 days after colitis induction. The distal colon segment was assessed macroscopically and microscopically. In addition, malondialdehyde (MDA) and nitric oxide (NO) levels of the colonic tissue and changes in body weight were measured.

Results

Macroscopic and microscopic examinations of colonic tissue samples showed morphological similarities to human Crohn’s disease (CD). The MDA and NO levels of the colonic tissues were significantly higher in the NaOH group compared to the acetic acid and sham control groups (P = 0.001).

Conclusion

NaOH may be used to induce Crohn’s colitis as an experimental model.

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Authors and Affiliations

  1. Department of Gastroenterology, Ankara Education and Research Hospital, Keklikpınarı mahallesi, 240 sokak, Duru apartmanı, 8/6, Dikmen, Çankaya, Ankara, Turkey

    Erdem Koçak, Seyfettin Köklü, Erdem Akbal & Adnan Taş

  2. Department of General Surgery, Ankara Education and Research Hospital, Ankara, Turkey

    Gökhan Karaca

  3. Department of Pathology, Ankara Education and Research Hospital, Ankara, Turkey

    Müzeyyen Hesna Astarcı

  4. Department of Biochemistry, Zonguldak Karaelmas University, Zonguldak, Turkey

    Berrak Güven & Murat Can

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  1. Erdem Koçak
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  2. Seyfettin Köklü
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  3. Erdem Akbal
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  4. Adnan Taş
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  5. Gökhan Karaca
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  7. Berrak Güven
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  8. Murat Can
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Correspondence to Erdem Koçak.

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Koçak, E., Köklü, S., Akbal, E. et al. NaOH-Induced Crohn’s Colitis in Rats: A Novel Experimental Model. Dig Dis Sci 56, 2833–2837 (2011). https://doi.org/10.1007/s10620-011-1697-8

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  • DOI: https://doi.org/10.1007/s10620-011-1697-8

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