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Naturally Occurring Glycoalkaloids in Potatoes Aggravate Intestinal Inflammation in Two Mouse Models of Inflammatory Bowel Disease

Digestive Diseases and Sciences volume 55, pages 3078–3085 (2010)Cite this article

Abstract

Background

Inflammatory bowel disease (IBD) may be initiated following disruption of the intestinal epithelial barrier. This disruption, in turn, permits luminal antigens unfettered access to the mucosal immune system and leads to an uncontrolled inflammatory response. Glycoalkaloids, which are found in potatoes, disrupt cholesterol-containing membranes such as those of the intestinal epithelium. Glycoalkaloid ingestion through potatoes may play a role in the initiation and/or perpetuation of IBD.

Aim

To determine if commercial and high glycoalkaloids containing fried potato skins aggravate intestinal inflammation using two different animal models of IBD.

Methods

Fried potato skins from commercial potatoes containing low/medium glycoalkaloid levels and high glycoalkaloids potatoes were fed for 20 days to interleukin 10 gene-deficient mice and dextran sodium sulfate-induced colitic mice. Intestinal permeability, mucosal cytokine and myeloperoxidase levels and body weight were determined to assess intestinal injury.

Results

Deep frying potato skins markedly increased glycoalkaloid content. Interleukin 10 gene-deficient mice fed fried commercial potato skins with medium glycoalkaloid content exhibited significantly elevated levels of ileal IFN-γ relative to controls. Mice in the dextran sodium sulfate colitis model that were fed the same strain of potatoes demonstrated significantly elevated levels of pro-inflammatory cytokines IFN-γ, TNF-α, and IL-17 in the colon in addition to an enhanced colonic permeability. Inflammatory response was intensified when the mice were fed potatoes with higher glycoalkaloid contents.

Conclusions

Our results demonstrate that consumption of potato skins containing glycoalkaloids can significantly aggravate intestinal inflammation in predisposed individuals.

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Acknowledgments

Funding

Crohn’s and Colitis Foundation of Canada (CCFC).

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Authors and Affiliations

  1. Division of Gastroenterology, University of Alberta, Zeidler Ledcor Building, Edmonton, AB, T6G 2X8, Canada

    Vadim Iablokov, Beate C. Sydora, Rae Foshaug, Jon Meddings & Richard N. Fedorak

  2. Department of Surgery, University of Alberta, Edmonton, AB, Canada

    Tom Churchill

  3. Alberta Agriculture and Rural Development, Brooks, AB, Canada

    Darcy Driedger

Authors
  1. Vadim Iablokov
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  2. Beate C. Sydora
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  3. Rae Foshaug
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  4. Jon Meddings
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  5. Darcy Driedger
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  6. Tom Churchill
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  7. Richard N. Fedorak
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Correspondence to Vadim Iablokov.

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Iablokov, V., Sydora, B.C., Foshaug, R. et al. Naturally Occurring Glycoalkaloids in Potatoes Aggravate Intestinal Inflammation in Two Mouse Models of Inflammatory Bowel Disease. Dig Dis Sci 55, 3078–3085 (2010). https://doi.org/10.1007/s10620-010-1158-9

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  • DOI: https://doi.org/10.1007/s10620-010-1158-9

Keywords

  • Glycoalkaloid
  • Inflammatory bowel disease
  • Crohn’s disease
  • Ulcerative colitis
  • Intestinal permeability
  • Mucosal inflammation