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Proinflammatory and Anti-inflammatory Cytokines Present in the Acute Phase of Experimental Colitis Treated with Saccharomyces boulardii

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Abstract

Purpose

To study the proinflammatory and anti-inflammatory cytokines present in the acute phase of trinitrobenzene sulfonic acid (TNBS)-induced experimental colitis treated with Saccharomyces boulardii.

Methods

Thirty male Wistar rats were divided into three groups: (1) treated group—received Saccharomyces boulardii for 14 days; (2) non-treated group—received sodium chloride solution for 14 days; (3) control group. Colitis was induced on the seventh day of the study in the treated and the non-treated groups using TNBS (10 mg) dissolved in 50% ethanol. Quantification of cytokines, including interleukin (IL)-1β (IL-1β), IL-6, transforming growth factor-β (TGF-β), IL-10 and tumor necrosis factor-α (TNF-α), in the serum and colonic tissue collected on day 14 were carried out using an enzyme-linked immunosorbent assay (ELISA).

Results

The mean concentrations of TGF-β in both the serum and the colonic tissue of the treated group were statistically higher than that of the control group. The mean concentration of TGF-β in the colonic tissue of the non-treated group was also statistically higher than the control group.

Conclusion

The group treated with Saccharomyces boulardii showed increased amounts of TGF-β, an anti-inflammatory cytokine, during the acute phase of colitis. There were no differences in the amount of TNF-α, IL-1β, IL-6, and IL-10 between the treated and the non-treated or the control groups during the acute phase of experimental colitis induced by TNBS.

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References

  1. Elson CO, Sartor RB, Tennyson G, Riddel R. Experimental models of IBD. Gastroenterology. 1995;109:1344–1367.

    Article  CAS  PubMed  Google Scholar 

  2. Sartor RB. Review article: how relevant to human inflammatory bowel disease are current animal models of intestinal inflammation? Aliment Pharmacol Ther. 1997;11(S3):89–97.

    PubMed  Google Scholar 

  3. Blumberg RS, Saubermann LJ, Strober W. Animal models of mucosal inflammation and their relation to human inflammatory bowel disease. Curr Opin Immunol. 1999;11:648–656.

    Article  CAS  PubMed  Google Scholar 

  4. Morris GP, Beck PL, Heridge MS, Depew WT, Szewczuk MR, Wallace JL. Hapten-induced models of chronic inflammation and ulceration in the rat colon. Gastroenterology. 1989;96:795–803.

    CAS  PubMed  Google Scholar 

  5. Cabezas ME, Gálvez J, Lorente MD, et al. Dietary fiber down-regulates colonic tumor necrosis factor α and nitric oxide production in trinitrobenzenesulfonic acid-induced colitic rats. J Nutr. 2002;132:3263–3271.

    Google Scholar 

  6. Cherbut C, Michel C, Lecannu G. The prebiotic characteristics of fructooligosaccharides are necessary for reduction of TNBS-induced colitis in rats. J Nutr. 2003;133:21–27.

    CAS  PubMed  Google Scholar 

  7. Peran L, Camuesco D, Comalada M, et al. Preventative effects of a probiotic, Lactobacillus salivarius ssp. Salivarius, in the TNBS model of rat colitis. World J Gastroenterol. 2005;11:5185–5192.

    PubMed  Google Scholar 

  8. Surawicz CM, Elmer GW, Speelman P, McFarland LV, Chinn J, Van Belle G. Prevention of antibiotic-associated diarrhea by Saccharomyces boulardii: a prospective study. Gastroenterology. 1989;96:981–988.

    CAS  PubMed  Google Scholar 

  9. McFarland LV, Surawicz CM, Greenberg RN, et al. Prevention of β-lactam-associated diarrhea by Saccharomyces boulardii compared with placebo. Am J Gastroenterol. 1995;90:439–448.

    CAS  PubMed  Google Scholar 

  10. McFarland LV, Surawicz CM, Greenberg RN, et al. A randomized placebo-controlled trial of Saccharomyces boulardii in combination with standard antibiotics for Clostridium difficile disease. JAMA. 1994;271:1913–1918.

    Article  CAS  PubMed  Google Scholar 

  11. Surawicz CM, McFarland LV, Greenberg RN, et al. The search for a better treatment for recurrent Clostridium difficile disease: use of high-dose vancomycin combined with Saccharomyces boulardii. Clin Infect Dis. 2000;31:1012–1017.

    Article  CAS  PubMed  Google Scholar 

  12. Buts JP, Corthier G, Delmee M. Saccharomyces boulardii for Clostridium difficile-associated enteropathies in infants. J Pediatr Gastroenterol Nutr. 1993;16:419–425.

    Article  CAS  PubMed  Google Scholar 

  13. Kurugöl Z, Koturoglu G. Effects of Saccharomyces boulardii in children with acute diarrhea. Acta Paediatr. 2005;94:44–47.

    Article  PubMed  Google Scholar 

  14. Kollaritsch H, Holst H, Grobara P, Wiedermann G. Prevention of traveler’s diarrhea with Saccharomyces boulardii. Results of a placebo-controlled double-blind study. Fortschr Med. 1993;111:152–156.

    CAS  PubMed  Google Scholar 

  15. Bléichner G, Blehaut H, Mentec H, Moyse D. Saccharomyces boulardii prevents diarrhea in critically ill tube-fed patients. A multicenter, randomized, double-blind placebo-controlled trial. Intensive Care Med. 1997;23:517–523.

    Article  PubMed  Google Scholar 

  16. Guslandi M, Mezzi G, Sorghi M, Testoni PA. Saccharomyces boulardii in maintenance treatment of Crohn’s disease. Dig Dis Sci. 2000;45:1462–1464.

    Article  CAS  PubMed  Google Scholar 

  17. Guslandi M, Giollo P, Testoni PA. A pilot trial of Saccharomyces boulardii in ulcerative colitis. Eur J Gastroenterol Hepatol. 2003;15:697–698.

    Article  PubMed  Google Scholar 

  18. Lee SK, Kim HJ, Chi SG, Kim YW. Saccharomyces boulardii ameliorates trinitrobenzenesulphonic acid-induced experimental colitis in rats: attenuation of pro-inflammatory gene expression. Gastrointest Endosc. 2006;63:AB 222.

    Google Scholar 

  19. Dalmasso G, Cottrez F, Imbert V, et al. Saccharomyces boulardii inhibits inflammatory bowel disease by trapping T cells in mesenteric lymph nodes. Gastroenterology. 2006;131:1812–1825.

    Article  CAS  PubMed  Google Scholar 

  20. Rogler G, Andus T. Cytokines in Inflammatory bowel disease. World J Surg. 1998;22:382–389.

    Article  CAS  PubMed  Google Scholar 

  21. Osman N, Adawi D, Adawi D, et al. Modulation of the effects of dextran sulfate sodium-induced acute colitis by the administration of different probiotic strains of Lactobacillus and Bifidobacterium. Dig Dis Sci. 2004;49:320–327.

    Article  CAS  PubMed  Google Scholar 

  22. Fuss IJ, Boirivant M, Lacy B, Strober W. The interrelated roles of TGF-β and IL-10 in the regulation of experimental colitis. J.Immunol. 2002;168:900–908.

    CAS  PubMed  Google Scholar 

  23. Torres MI, Garcia-Martin M, Fernandez MI, Nieto N, Gil A, Rios A. Experimental colitis induced by trinitrobenzenesulfonic acid: an ultrastructural and histochemical study. Dig Dis Sci. 1999;44:2523–2529.

    Article  CAS  PubMed  Google Scholar 

  24. Lamine F, Eutamene H, Fioramonti J, Bueno L, Theodorou V. Colonic responses to Lactobacillus farciminis treatment in trinitrobenzene sulphonic acid-induced colitis in rats. Scand J Gastroenterol. 2004;39:1250–1258.

    Article  CAS  PubMed  Google Scholar 

  25. Foligné B, Nutten S, Steidler L, et al. Recommendations for improved use of the murine TNBS-induced colitis model in evaluating anti-inflammatory properties of lactic acid bacteria: technical and microbiological aspects. Dig Dis Sci. 2006;51:390–400.

    Article  PubMed  Google Scholar 

  26. Sugimoto K, Hanai H, Tozawa K, et al. Curcumin prevents and ameliorates trinitrobenzene sulfonic acid–induced colitis in mice. Gastroenterology. 2002;123:1912–1922.

    Article  CAS  PubMed  Google Scholar 

  27. Coeuret V, Gueguen M, Vernoux JP. Numbers and strains of lactobacilli in some probiotic products. Int J Food Microbiol. 2004;97:147–156.

    Article  PubMed  Google Scholar 

  28. Szajewska H, Setty M, Mrukowicz J, Guandalini S. Probiotics in gastrointestinal diseases in children: hard and not-so-hard evidence of efficacy. J Pediatr Gastroenterol Nutr. 2006;42:454–475.

    Article  PubMed  Google Scholar 

  29. Penna FJ, Filho LA, Calçado AC, Junior HR, Nicolli JR. Up-to-date clinical and experimental basis for the use of probiotics. J Pediatr (Rio J). 2000;76(Suppl 1):S209–S217.

    Google Scholar 

  30. Isolauri E, Kirjavainen PV, Salminen S. Probiotics: a role in the treatment of intestinal infection and inflammation? Gut. 2002;50(Suppl 3):III54–III59.

    Article  PubMed  Google Scholar 

  31. Neurath MF, Fuss I, Kelsall BL, Presky DH, Waegell W, Strober W. Experimental granulomatous colitis in mice is abrogated by induction of TGF-beta-mediated oral tolerance. J Exp Med. 1996;183:2605–2616.

    Article  CAS  PubMed  Google Scholar 

  32. Mulsow JJ, Watson RW, Fitzpatrick JM, O’Connell PR. Transforming growth factor-beta promotes pro-fibrotic behavior by serosal fibroblasts via PKC and ERK1/2 mitogen activated protein kinase cell signaling. Ann Surg. 2005;242:880–889.

    Article  PubMed  Google Scholar 

  33. Monteleone I, Pallone F, Monteleone G. Interleukin-23 and Th17 cells in the control of gut inflammation. Mediators Inflamm. 2009;2009:297645.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Division of Pediatric Gastroenterology, Federal University of Sao Paulo, Sao Paulo, Brazil

    Nathália Nahas Grijó & Vera Lucia Sdepanian

  2. São Leopoldo Mandic Dental Research Center, Campinas, SP, Brazil

    Ricardo Carneiro Borra

  3. Rua dos Otonis 880, Apt 102, CEP: 04025-002, São Paulo, SP, Brazil

    Vera Lucia Sdepanian

Authors
  1. Nathália Nahas Grijó
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  2. Ricardo Carneiro Borra
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  3. Vera Lucia Sdepanian
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Correspondence to Vera Lucia Sdepanian.

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Grijó, N.N., Borra, R.C. & Sdepanian, V.L. Proinflammatory and Anti-inflammatory Cytokines Present in the Acute Phase of Experimental Colitis Treated with Saccharomyces boulardii . Dig Dis Sci 55, 2498–2504 (2010). https://doi.org/10.1007/s10620-009-1072-1

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  • DOI: https://doi.org/10.1007/s10620-009-1072-1

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