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Lactobacillus plantarum Prevents the Upregulation of Adhesion Molecule Expression in an Experimental Colitis Model

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Abstract

Background

Lactobacillus consumption has been shown to attenuate the severity of experimental colitis. Whether the effects of Lactobacillus on colitis are related to modulation of leukocyte recruitment into the inflamed intestine is unclear.

Aims

To investigate the effect of Lactobacillus plantarum daily intragastric administration on lymphocyte homing and intestinal inflammation in interleukin 10 (IL-10) knockout mice, an experimental model of colitis.

Methods

Two groups of ten IL-10 knockout mice were fed phosphate buffered saline containing Lactobacillus plantarum 1258 or unmodified vehicle for 4 weeks. Two groups of ten wild-type mice were used as controls. At killing, the bowels were histologically scored and evaluated by transmission electron microscopy. Mucosal addressin cell adhesion molecule 1 (MAdCAM-1) and intercellular adhesion molecule 1 (ICAM-1) expression were determined by immunohistochemistry. The Levels of proinflammatory cytokines, tumor necrosis factor α (TNF-α), and interferon γ (IFN-γ) were determined by ELISA. In addition, levels of CD3, α4β7, ICAM-1, and MAdCAM-1 were determined by reverse-transcription polymerase chain reaction and Western blot.

Results

L. plantarum treatment improved the histological damage score in KO mice compared to untreated KO mice. L. plantarum significantly attenuated the expression of MAdCAM-1, ICAM-1, CD3, and α4β7, but did not affect the levels of TNF-α and IFN-γ when treated KO mice were compared to untreated KO mice.

Conclusions

L. plantarum interfered with the upregulation of adhesion molecules observed in IL-10 knockout mice compared to wild-type mice, attenuating the symptoms of colitis.

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Acknowledgments

This work was supported by the National Key Basic Research Plan Program of China (973 Program), No. 2008CB517403, and the Special Foundation for Academician Jie-Shou Li (Intestinal Barrier Research), No. LJS_2009001.

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Authors and Affiliations

  1. Department of Surgery, The Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University, 600 Yishan Road, 200233, Shanghai, China

    Zhao-Xin Chu, Hong-Qi Chen, Yan-Lei Ma, Yu-Kun Zhou, Ming Zhang, Peng Zhang & Huan-Long Qin

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Correspondence to Huan-Long Qin.

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Chu, ZX., Chen, HQ., Ma, YL. et al. Lactobacillus plantarum Prevents the Upregulation of Adhesion Molecule Expression in an Experimental Colitis Model. Dig Dis Sci 55, 2505–2513 (2010). https://doi.org/10.1007/s10620-009-1063-2

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