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Intestinal Ischemic Preconditioning After Ischemia/Reperfusion Injury in Rat Intestine: Profiling Global Gene Expression Patterns

Digestive Diseases and Sciences volume 55, pages 1866–1877 (2010)Cite this article

Abstract

Objective

Intestinal ischemia/reperfusion (IR) injury involves activation of inflammatory mediators, mucosal necrosis, ileus, and alteration in a variety of gene products. Ischemic preconditioning (IPC) reduced all the effects of intestinal injury seen in IR. In an effort to investigate the molecular mechanisms responsible for the protective effects afforded by IPC, we sought to characterize the global gene expression pattern in rats subjected to IPC in the setting of IR injury.

Methods

Rats were randomized into five groups: (1) Sham, (2) IPC only (3) IR, (4) Early IPC + IR (IPC → IR), and (5) Late IPC + IR (IPC → 24 h → IR). At 6 h after reperfusion, ileum was harvested for total RNA isolation, pooled, and analyzed on complementary DNA (cDNA) microarrays with validation using real-time polymerase chain reaction (PCR). Significance Analysis of Microarray (SAM) software was used to determine statistically significant changes in gene expression.

Results

Early IPC + IR had 5,167 induced and 4 repressed genes compared with the other groups. SAM analysis revealed 474 out of 10,000 genes differentially expressed among the groups. Early and Late IPC + IR had more genes involved in redox hemostasis, the immune/inflammatory response, and apoptosis than either the IPC only or IR alone groups.

Conclusion

The transcriptional profile suggests that IPC exerts its protective effects by regulating the gene response to injury in the intestine.

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Acknowledgments

This work was supported by NIGMS grants T32 GM 08792, P50 GM38529, the Department of Defense “DREAMS” grant (B.C.K.), and endowment funds from The James T. and Nancy B. Willerson Chair (B.C.K.).

Author information

Affiliations

  1. Department of Pediatric Surgery, University of Texas Medical School at Houston, 6431 Fannin Street, Suite 5.246, Houston, TX, 77030, USA

    Stacey D. Moore-Olufemi, Fernando Jimenez & Charles S. Cox Jr.

  2. Department of Surgery, University of Texas Medical School at Houston, Houston, TX, USA

    Norio Sato, Rosemary A. Kozar, Frederick A. Moore, Ravi S. Radhakrishnan & Shinil Shah

  3. Department of Internal Medicine, University of Texas Medical School at Houston, Houston, TX, USA

    Bruce C. Kone

  4. Department of Integrative Biology and Pharmacology, University of Texas Medical School at Houston, Houston, TX, USA

    Bruce C. Kone

  5. Department of Trauma Research Center, University of Texas Medical School at Houston, Houston, TX, USA

    Stacey D. Moore-Olufemi, Norio Sato, Rosemary A. Kozar, Frederick A. Moore, Ravi S. Radhakrishnan, Bruce C. Kone & Charles S. Cox Jr.

  6. The Brown Foundation Institute of Molecular Medicine for the Prevention of Human Diseases, Houston, TX, USA

    Bruce C. Kone

  7. Department of Cancer Genetics, M.D. Anderson Cancer Center, Houston, TX, USA

    Shodimu-Emmanuel Olufemi

  8. Department of Molecular Genetics, M.D. Anderson Cancer Center, Houston, TX, USA

    Steve Lott

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  1. Stacey D. Moore-Olufemi
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  2. Shodimu-Emmanuel Olufemi
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  3. Steve Lott
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  6. Frederick A. Moore
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  8. Shinil Shah
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  9. Fernando Jimenez
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  10. Bruce C. Kone
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Correspondence to Charles S. Cox Jr..

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Moore-Olufemi, S.D., Olufemi, SE., Lott, S. et al. Intestinal Ischemic Preconditioning After Ischemia/Reperfusion Injury in Rat Intestine: Profiling Global Gene Expression Patterns. Dig Dis Sci 55, 1866–1877 (2010). https://doi.org/10.1007/s10620-009-0980-4

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  • DOI: https://doi.org/10.1007/s10620-009-0980-4

Keywords

  • Ischemia/reperfusion
  • Gene expression
  • Intestine
  • Preconditioning