Abstract
MicroRNAs are a group of small non-coding RNAs with modulator activity of gene expression. Recent studies have uncovered a profound role of microRNAs in liver diseases. This study aimed to investigate a potential relationship between microRNA-223 (miR-223) expression and hepatic ischemia/reperfusion injury in mice. Quantitative RT-PCR analysis showed that miR-223 expression levels were greatly up-regulated in the livers after 75 min ischemia followed by 120 min reperfusion when compared to sham controls (2.59 ± 0.23 vs. 0.83 ± 0.15; P < 0.01). Correlation analysis also revealed that hepatic miR-223 expression level was significantly positively correlated with serum markers of ischemic injury. By prediction assay of miRNA targets mRNA, acyl-CoA synthetase long-chain family member 3, ephrin A1, and ras homolog gene family member B were predicted to be downstream targets of miR-223. Thus, we conclude that hepatic ischemia/reperfusion injury might be another form of liver disease that is associated with alteration in miR-223 expression.
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Acknowledgments
The authors thank Ms. Qiao-Juan Shi for her help in animal experiments and Drs. Li-Ming Xu and Ding Wei for their assistance in histological analysis. This study was supported by the National Natural Science Foundation of China (No. 30571804).
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Chao-Hui Yu and Cheng-Fu Xu contributed equally to this work.
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Yu, CH., Xu, CF. & Li, YM. Association of MicroRNA-223 Expression with Hepatic Ischemia/Reperfusion Injury in Mice. Dig Dis Sci 54, 2362–2366 (2009). https://doi.org/10.1007/s10620-008-0629-8
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DOI: https://doi.org/10.1007/s10620-008-0629-8