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An Anti-adenoma Antibody, Adnab-9, May Reflect the Risk for Neoplastic Progression in Familial Hamartomatous Polyposis Syndromes

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Abstract

Patients with the hamartomatous polyposis Peutz-Jeghers and familial juvenile polyposis syndromes are predisposed to colorectal cancer but lack early genetic alterations found in adenomatous premalignant lesions. We studied hamartomatous polyps for the expression of an early preneoplastic colorectal neoplasia risk marker also found in familial adenomatous polyposis patients. Retrospective, genetic, and hospital archival tissue immunohistochemistry using Adnab-9, a premalignant marker often found in Paneth-like cells (PCs), was performed on sections of polyps from eight patients with Peutz-Jeghers syndrome, eight patients with familial juvenile polyposis, and 36 hyperplastic polyp control sections. Anti-α-defensin 5 (AD5), a universal PC marker, was also used to label a subgroup of sections. Hamartomatous polyposis patients also underwent specific genetic analysis. Eighty-nine percent of Peutz-Jeghers syndrome polyps labeled with Adnab-9 compared with 63% for AD5; 88% of familial juvenile polyposis sections also labeled with Adnab-9. Of the 36 hyperplastic polyp sections, only four (11%) labeled with Adnab-9 and one (3%) with AD5. Adnab-9 labeling of PCs in the epithelial elements of hamartomatous colonic lesions of hereditary hamartomatous syndrome patients reflects the predisposition to colorectal cancer, further justifying early intervention strategies.

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Abbreviations

APC:

Adenomatous polyposis coli

CRC:

Colorectal cancer

AEC:

3-amino-9-ethyl-carbazole

FJP:

Hereditary familial juvenile polyposis syndrome

IBD:

Inflammatory bowel disease

OD:

Optical densitometry

PJS:

Peutz-Jeghers syndrome

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Acknowledgements

The authors would like to express their gratitude to Susan Katz, BA, for assistance with specimen collection. The work was supported in part by a Department of Veterans Affairs (VAMC) grant awarded to Dr. Martin Tobi, a Kaiser Permanente Research Foundation grant to Dr. Michael Lawson, and a VAMC Merit Review Grant to Dr. Russell Jacoby. This paper is dedicated to the memory of Irwin Stein.

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Authors and Affiliations

  1. John D. Dingell VAMC Departments of Medicine, Gastroenterology and Pathology, Wayne State University School of Medicine, Detroit, MI, USA

    Martin Tobi, Michael Kam, Nadeem Ullah, Kashif Qureshi, Violeta Yordanova, James Hatfield, Suzanne E. G. Fligiel & Paula Sochacki

  2. Section Gastroenterology MED III/GI, Philadelphia VAMC, Philadelphia, PA, 19104, USA

    Martin Tobi

  3. Department of Medicine, The Milton S. Hershey Medical Center, The Pennsylvania State University College of Medicine, Hershey, PA, USA

    Thomas McGarrity

  4. Wm. S. Middelton Memorial VAMC, University Wisconsin Comprehensive Cancer Center, Madison, WI, USA

    Carolyn Cole & Russell Jacoby

  5. Kaiser Permanente Medical Group, Sacramento, CA, USA

    Michael Lawson

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  1. Martin Tobi
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  2. Michael Kam
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  3. Nadeem Ullah
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  4. Kashif Qureshi
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  5. Violeta Yordanova
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  7. Suzanne E. G. Fligiel
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  8. Paula Sochacki
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  9. Thomas McGarrity
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  10. Carolyn Cole
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  11. Michael Lawson
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  12. Russell Jacoby
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Correspondence to Martin Tobi.

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Tobi, M., Kam, M., Ullah, N. et al. An Anti-adenoma Antibody, Adnab-9, May Reflect the Risk for Neoplastic Progression in Familial Hamartomatous Polyposis Syndromes. Dig Dis Sci 53, 723–729 (2008). https://doi.org/10.1007/s10620-007-9947-5

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  • DOI: https://doi.org/10.1007/s10620-007-9947-5

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