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Application of Tissue Microarrays to Study the Influence of Dexamethasone on NF-κB Expression of Pancreas in Rat with Severe Acute Pancreatitis

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Abstract

To discuss the influence of dexamethasone on NF-κB expression of pancreas in rat with severe acute pancreatitis (SAP). Ninety rat SAP models were divided into the model group and dexamethasone treatment group with 45 rats in each group; another healthy 45 rats were selected to be the sham operation group. The groups were divided into the 3, 6 and 12 h group with 15 rats in each group. The survivals, pancreas pathological changes were observed 3, 6 and 12 h after operation. The changes in expression levels of NF-κB protein of pancreas tissue microarray were observed. The treatment group was significantly lower than the model group at 3 and 6 h (P < 0.05) and than the model group at 12 h in pancreas pathological scores (P < 0.01). The expression level of NF-κB protein of pancreas head of the treatment group was significantly less than that of the model group at 3 h (P < 0.01). The alleviation of pancreatic tissue injury by dexamethasone during SAP might be closely related to its role in inhibiting NF-κB expression and regulating cytokines. The advantages of tissue microarrays in pancreatitis pathological examination include time and energy savings, high efficiency and representative results.

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Acknowledgments

This work was supported by technological foundation project of Traditional Chinese Medicine Science of Zhejiang province (nos. 2003C130 and 2004C142), the foundation project for medical science and technology of Zhejiang province (no. 2003B134), the Grave foundation project for technological and development of Hangzhou (no. 2003123B19), the intensive foundation project for technology of Hangzhou (no. 2004Z006), the foundation project for medical science and technology of Hangzhou (no. 2003A004), and the foundation project for technology of Hangzhou (no. 2005224). The authors are grateful to Chen Li and Tian hua, whose institution is second affiliated hospital of school of medicine, Zhejiang University, for their technical assistance.

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Authors and Affiliations

  1. Department of General Surgery, Hangzhou First People’s Hospital, 261 Huansha Road, Hangzhou, Zhejiang Province, 310006, China

    Xi Ping Zhang

  2. Class s0201 of Seven Year’s Clinical Medicine, Shanxi Medical University, Taiyuan, Shanxi Province, 310001, China

    Ling Zhang

  3. Zhejiang University of Traditional Chinese Medical, Hangzhou, Zhejiang Province, 310053, China

    Hong Miao Xu

  4. Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, 310006, China

    Yan Ping Xu

  5. Department of Gynaecology and Obstetrics, Hangzhou First People’s Hospital, Hangzhou, Zhejiang Province, 310006, China

    Qi Hui Cheng

  6. Supply room, Hangzhou First People’s Hospital, Hangzhou, Zhejiang Province, 310006, China

    Jian Mei Wang

  7. Operating Room, Hangzhou First People’s Hospital, Hangzhou, Zhejiang Province, 310006, China

    Hai Ping Shen

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  1. Xi Ping Zhang
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Zhang, X.P., Zhang, L., Xu, H.M. et al. Application of Tissue Microarrays to Study the Influence of Dexamethasone on NF-κB Expression of Pancreas in Rat with Severe Acute Pancreatitis. Dig Dis Sci 53, 571–580 (2008). https://doi.org/10.1007/s10620-007-9867-4

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