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Microcirculatory Changes in Experimental Mesenteric Longitudinal Ulcers of the Small Intestine in Rats

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Abstract

Rats receiving intracolonic administration of indomethacin develop longitudinal ulcers on the mesenteric side of the small intestine that are similar to those seen in the acute phase of Crohn's disease. To investigate the causative role of microcirculatory disturbances and to elucidate the therapeutic effect of antioxidants on this enteropathy in rats, we serially evaluated changes in regional blood flow of the small intestine using laser Doppler perfusion imaging and the colored microsphere injection method. Both methods disclosed stepwise hyperperfusion limited to the mesenteric side of the small intestine following transient ischemia during the initial 30–60 minutes. In addition, both a radical scavenger and a radical production inhibitor significantly ameliorated the mesenteric longitudinal ulcers. We concluded that ischemia–reperfusion on the mesenteric side accompanying excessive production of radicals might be strongly involved in indomethacin-induced longitudinal ulcers of the small intestine in rats.

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Acknowledgments

The authors thank Mr. Katsunori Ohta, Medical Experimental Center, Kawasaki Medical School, for his help with measurement of intestinal blood flow and Professor David H. Waterbury, Department of Foreign Languages, Kawasaki Medical School, for correcting the English used in this manuscript. We also would like to thank the personnel of the Electron Microscopy Center, the Laboratory Animal Center, the Biochemical Center, the Physiological Function Research Center, the Tissue Culture and Immunology Center and the Medical Experimental Center, Kawasaki Medical School, for assistance in our experiments.

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Authors and Affiliations

  1. Division of Gastroenterology, Department of Medicine, Kawasaki Medical School, Kurashiki, Japan

    Kayoko Shimizu, Hideki Koga & Ken Haruma

  2. Department of Medicine and Clinical Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan

    Hideki Koga & Mitsuo Iida

  3. Division of Gastroenterology, Department of Medicine, Kawasaki Medical School, 577 Matsushima, Kurashiki, 701-0192, Japan

    Kayoko Shimizu

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  1. Kayoko Shimizu
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  2. Hideki Koga
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  4. Ken Haruma
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Correspondence to Kayoko Shimizu.

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Shimizu, K., Koga, H., Iida, M. et al. Microcirculatory Changes in Experimental Mesenteric Longitudinal Ulcers of the Small Intestine in Rats. Dig Dis Sci 52, 3019–3028 (2007). https://doi.org/10.1007/s10620-007-9804-6

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  • DOI: https://doi.org/10.1007/s10620-007-9804-6

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