Abstract
Metallothionein (MT) expression was investigated in pregnant mice infected with H. pylori or H. felis and their fetuses and pups. Mice, healthy or infected with H. pylori or H. felis (n = 18/group), were sacrificed 2 weeks after impregnation or 4 weeks postpartum. Pups were sacrificed as fetuses, after birth, or at ages 11 or 28 days. Whole fetuses, stomachs, small intestines, and livers were assayed for MT. MT was increased (P<0.05) by two- and threefold in fetuses from H. pylori- and H. felis-infected mothers, respectively, compared to control fetuses. Stomach MT of H. felis-infected pregnant mice, and newborns and 28-day pups from H. felis-infected mothers, was elevated (P<0.05) twofold compared to that of control mice and pups. Liver MT was decreased (P<0.05) in H. felis-infected mice 4 weeks postpartum (18%) and in their 11-day (69%) and 28-day (53%) pups, while small intestinal MT was decreased in H. felis-infected pregnant mice (17%), H. felis-infected mice 4 weeks postpartum (19%), and their 11-day pups (35%), compared to control mice. H. felis infection altered MT levels of pregnant mice, their fetuses and pups, and mice postpartum, which may be a response to the marked inflammation.
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References
Sanders MK, Peura DA (2002) Helicobacter pylori-associated diseases. Curr Gastroenterol Rep 4:448–454
Bjorkholm B, Falk P, Engstrand L, Nyren O (2003) Helicobacter pylori: resurrection of the cancer link. J Intern Med 253:102–119
Dundon WG, Nishioka H, Polenghi A, Papinutto E, Zanotti G, Montemurro P, Del GG, Rappuoli R, Montecucco C (2002) The neutrophil-activating protein of Helicobacter pylori. Int J Med Microbiol 291:545–550
Gotz JM, Thio JL, Verspaget HW, Offerhaus GJ, Biemond I, Lamers CB, Veenendaal RA (1997) Treatment of Helicobacter pylori infection favourably affects gastric mucosal superoxide dismutases. Gut 40:591–596
Gotz JM, van Kan CI, Verspaget HW, Biemond I, Lamers CB, Veenendaal RA (1996) Gastric mucosal superoxide dismutases in Helicobacter pylori infection. Gut 38:502–506
Noguchi K, Kato K, Moriya T, Suzuki T, Saito M, Kikuchi T, Yang J, Imatani A, Sekine H, Ohara S, Toyota T, Shimosegawa T, Sasano H (2002) Analysis of cell damage in Helicobacter pylori-associated gastritis. Pathol Int 52:110–118
Shirin H, Pinto JT, Liu LU, Merzianu M, Sordillo EM, Moss SF (2001) Helicobacter pylori decreases gastric mucosal glutathione. Cancer Lett 164:127–133
Viarengo A, Burlando B, Ceratto N, Panfoli I (2000) Antioxidant role of metallothioneins: a comparative overview. Cell Mol Biol 46:407–417
Maret W, Vallee BL (1998) Thiolate ligands in metallothionein confer redox activity on zinc clusters. Proc Natl Acad Sci USA 95:3478–3482
Tran CD, Huynh H, Campbell MAF, Van Den Berg M, Van Der Pas M, Philcox JC, Coyle P, Rofe AM, Butler RN (2003) Helicobacter-induced gastritis in mice not expressing metallothionein-I &II. Helicobacter 8:533–541
Tran CD, Campbell MAF, Kolev Y, Chamberlain S, Huynh HQ, Butler RN (2005) Short-term zinc supplementation attenuates Helicobacter felis-induced gastritis in the mouse. J Infect 50:417–424
Lanciers S, Despinasse B, Mehta DI, Blecker UR (1999) Increased susceptibility to Helicobacter pylori infection in pregnancy. Infect Dis Obstet Gynecol 7:195–198
Eaton DL, Toal BF (1982) Evaluation of Cd/hemoglobin affinity assay for rapid determination of metallothionein in biological tissues. Toxicol Appl Pharmacol 66:134–142
Klinowski E, Broide E, Varsano R, Eshchar J, Scapa E (1996) Superoxide dismutase activity in duodenal ulcer patients. Eur J Gastroenterol Hepatol 8:1151–1155
Kountouras J, Chatzopoulos D, Zavos C (2000) Reactive oxygen metabolites and upper gastrointestinal diseases. Hepatogastroenterology 48:743–751
Coyle P, Philcox JC, Carey LC, Rofe AM (2002) Metallothionein: the multipurpose protein. Cell Mol Life Sci 59:627–647
De SK, McMaster MT, Dey SK, Andrews GK (1989) Cell-specific metallothionein gene expression in mouse decidua and placentae. Development 107:611–621
Andrews GK, Adamson ED, Gedamu L (1984) The ontogeny of expression of murine metallothionein: comparison with the alpha-fetoprotein gene. Dev Biol 103:294–303
Carey LC, Coyle P, Philcox JC, Rofe AM (2000) Maternal ethanol exposure is associated with decreased plasma zinc and increased fetal abnormalities in normal but not metallothionein-null mice. Alcohol Clin Exp Res 24:213–219
Minoura T, Kato S, Otsu S, Fujioka T, Iinuma K, Nishizono A (2003) Childhood Helicobacter pylori infection in a murine model: maternal transmission and eradication by systemic immunization using bacterial antigen-aluminium hydroxide. Clin Exp Immunol 134:32–37
Mannick EE, Schurr JR, Zapata A, Lentz JJ, Gastanaduy M, Cote RL, Delgado A, Correa P, Correa H (2004) Gene expression in gastric biopsies from patients infected with Helicobacter pylori. Scand J Gastroenterol 39:1192–1200
Acknowledgments
This work was supported by the National Health and Medical Research Council Industry Fellowship to Dr Tran. The work was performed at the Gastroenterology Unit, Children, Youth and Women’s Health Service, North Adelaide, SA, Australia.
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Tran, C.D., Gøbel, R. & Symonds, E.L. Metallothionein Expression in Helicobacter-Infected Pregnant Mice and Their Fetuses and Pups. Dig Dis Sci 52, 1527–1532 (2007). https://doi.org/10.1007/s10620-006-9611-5
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DOI: https://doi.org/10.1007/s10620-006-9611-5