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High Proportions of Proinflammatory Bacteria on the Colonic Mucosa in a Young Patient with Ulcerative Colitis as Revealed by Cloning and Sequencing of 16S rRNA Genes

Digestive Diseases and Sciences volume 52, pages 620–627 (2007)Cite this article

Abstract

The pathogenesis of ulcerative colitis (UC) remains unknown. It is thought to be due to an abnormal and uncontrolled immune response to normally occurring constituents of the intestine. Microbial agents appear to be involved in the pathogenesis and intestinal bacteria seem to be an important factor in the development and chronicity. The aim of this study was to investigate the colonic microbiota of a patient with UC. The colonic tissues were taken during surgery from a 12-year-old girl suffering from UC. The microbiota on the colonic samples was studied by cloning and sequencing of amplified 16S rRNA genes. Compared with healthy subjects, alteration of the dominant bacterial group was observed in the UC patient. We found a high incidence of Enterobacteriaceae, Bacteroides fragilis, and the single phylotype of the Faecalibacterium prausnitzii-like “Butyrate-producing bacterium” L2-6. Furthermore, there was a substantial presence of Pseudomonas aeruginosa in the present case of UC. The high proportion of adverse proinflammatory species is striking in the present case compared with more normal situations. Even if those bacteria are not the cause of the UC, they most probably enhance the symptoms of the disease.

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Acknowledgment

The authors thank Bertil Pettersson at the Department of Biotechnology, Royal Institute of Technology, Sweden, for sequencing bacterial clones.

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Affiliations

  1. Department of Food Technology, Engineering and Nutrition, Lund University, 221 00 Lund, Sweden

    Mei Wang, Göran Molin & Siv Ahrné

  2. Department of Surgery, University Hospital Malmö, Lund University, S-205 02, Malmö, Sweden

    Diya Adawi & Bengt Jeppsson

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  1. Mei Wang
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  2. Göran Molin
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  3. Siv Ahrné
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  4. Diya Adawi
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  5. Bengt Jeppsson
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Correspondence to Bengt Jeppsson.

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Wang, M., Molin, G., Ahrné, S. et al. High Proportions of Proinflammatory Bacteria on the Colonic Mucosa in a Young Patient with Ulcerative Colitis as Revealed by Cloning and Sequencing of 16S rRNA Genes. Dig Dis Sci 52, 620–627 (2007). https://doi.org/10.1007/s10620-006-9461-1

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  • DOI: https://doi.org/10.1007/s10620-006-9461-1

Keywords

  • Ulcerative colitis
  • Colonic microbiota
  • 16S rRNA gene
  • Cloning and sequencing
  • Inflammatory bowel disease