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Polymeric Formula Has Direct Anti-Inflammatory Effects on Enterocytes in an in VitroModel of Intestinal Inflammation

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Abstract

Exclusive enteral nutrition using polymeric formula (PF) is a well-established therapeutic option for active Crohn’s disease; however, its mechanisms of action are unknown. We investigated the anti-inflammatory effects of PF in an in vitro model of epithelial cell inflammation. PF did not affect cell viability over a range of dilutions, but when PF was added to the culture medium the interleukin (IL)-8 response to proinflammatory stimuli was significantly reduced. This effect was due to PF acting directly on the cells as the IL-8 response was still reduced when PF was separated from the proinflammatory stimuli in a 2-compartment system. In the presence of PF, nuclear factor (NF)-κB nuclear migration was not inhibited; however, IκBα degradation was delayed. PF has direct anti-inflammatory effects upon immortalized colonic enterocytes. Therefore PF may, in part, modulate gut inflammation by directly reducing the inflammatory response of the intestinal epithelium.

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Authors and Affiliations

  1. School of Women’s and Children’s Health, University of New South Wales, Sydney, Australia

    Naomi S. H. de Jong, Steven T. Leach & Andrew S. Day

  2. Department of Gastroenterology, Sydney Children’s Hospital, High Street, Randwick, New South Wales, 2031, Australia

    Andrew S. Day

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  1. Naomi S. H. de Jong
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  2. Steven T. Leach
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  3. Andrew S. Day
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Correspondence to Andrew S. Day.

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de Jong, N.S.H., Leach, S.T. & Day, A.S. Polymeric Formula Has Direct Anti-Inflammatory Effects on Enterocytes in an in VitroModel of Intestinal Inflammation. Dig Dis Sci 52, 2029–2036 (2007). https://doi.org/10.1007/s10620-006-9449-x

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  • DOI: https://doi.org/10.1007/s10620-006-9449-x

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