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Heat Shock Preconditioning Induces Protein Carbonylation and Alters Antioxidant Protection in Superficially Injured Guinea Pig Gastric Mucosa In Vitro

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Abstract

According to our previous studies, heat shock preconditioning of gastric mucosa requires modulation of protein synthesis and eicosanoid pathways to induce protection against superficial injury. This may be caused by heat shock–induced oxidative stress. We studied the effect of heat shock preconditioning with normothermic recovery on redox status in superficially injured (1.25 mmol NaCl for 5 min) Ussing chamber perfused guinea pig gastric mucosa allowed to recover for 3 hr after injury. Protein oxidation, lipid peroxidation, level of superoxide dismutase, level of heat shock protein 72 (HSP72), and level of oxygen radical absorbance capacity were measured. Superficial injury increased lipid peroxidation. Heat shock preconditioning decreased oxygen radical absorbance capacity and increased protein carbonyl and HSP72 levels, but inhibited electrophysiologic recovery. Exposure to indomethacin and arachidonic acid (AA) partially abolished this pro-oxidative and inhibitory effect on recovery, but maintained HSP72 levels and decreased protein carbonyls, lipid peroxidation, and oxygen radical absorbance capacity. In conclusion, superficial injury increased lipid peroxidation. Heat shock preconditioning alone induced oxidative stress via indomethacin- and AA-sensitive mechanisms. The development of optimal cytoprotective strategy may therefore require control of oxidative stress and modulation of the eicosanoid pathways.

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Acknowledgements

This work has been supported by The Mary and Georg C. Ehrnrooth Foundation; The Pirkanmaa Regional Fund of Finnish Cultural Foundation; Finnish Ministry of Education; Yrjö Jahnsson Foundation, Helsinki, Finland; and High Technology Foundation of Eastern Finland. We thank Ms. Taina Simonen and Taija Vaarala for skillful technical assistance.

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Authors and Affiliations

  1. Department of Surgery, Kuopio University Hospital, Kuopio, Finland

    Niku K. J. Oksala & Esko Alhava

  2. Department of Surgery, Helsinki University Central Hospital, Jorvi Hospital, Helsinki, Finland

    Hannu Paimela

  3. Department of Physiology, Institute of Biomedicine, University of Kuopio, Kuopio, Finland

    Niku K. J. Oksala & Mustafa Atalay

  4. Department of Surgery, Tampere University Hospital, Teiskontie 35, P.O. Box 2000, FIN-33521, Tampere, Finland

    Niku K. J. Oksala

  5. Department of Surgery, w Hospital, Narvik, Norway

    Hannu Paimela

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  1. Niku K. J. Oksala
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Correspondence to Niku K. J. Oksala.

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Oksala, N.K.J., Paimela, H., Alhava, E. et al. Heat Shock Preconditioning Induces Protein Carbonylation and Alters Antioxidant Protection in Superficially Injured Guinea Pig Gastric Mucosa In Vitro. Dig Dis Sci 52, 1897–1905 (2007). https://doi.org/10.1007/s10620-006-9214-1

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