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Effect of Rebamipide on the Colonic Barrier in Interleukin-10-Deficient Mice

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Abstract

Our aim was to study the effect of a mucosal protective agent, rebamipide, on the colonic barrier and the immune response in colitis-prone interleukin-10-deficient (IL-10−/−) C57BL/6 mice infected with Helicobacter hepaticus. After sacrifice, in all mice, control, or previously infected with H. hepaticus, or previously infected and treated with rebamipide enema, a histological examination of colonic samples was performed, intestinal permeability was studied in Ussing chamber, and mesenteric lymph node proliferation and cytokine secretion were measured. Mice treated with rebamipide presented a reinforcement of the distal colonic epithelial barrier, an increase of mesenteric lymph node cells proliferation, and of IFNγ and IL-12 secretion. These results indicate that in IL-10−/− mice with mild colitis, rectally administered rebamipide reinforces the distal colonic barrier and has a slight Th1 immuno-stimulatory effect on mesenteric lymph node cells. These properties could be helpful in the management of some inflammatory bowel diseases.

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Acknowledgment

This work was supported by Otsuka Pharmaceutical, Tokyo, Japan.

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Authors and Affiliations

  1. INSERM-ERI 10, Université Victor Segalen, Bordeaux, France

    David Laharie, Corinne Asencio, Frank Zerbib & Francis Mégraud

  2. INSERM U793, Faculté Necker-René Descartes, Bordeaux, France

    Sandrine Ménard, Teresita Vidal-Martinez, Céline Candalh, Martine Heyman & Tamara Matysiak-Budnik

  3. Service d’Anatomo-pathologie, Hôpital Pellegrin, Bordeaux, France

    Anne Rullier

  4. INSERM U793, Faculté Necker-René Descartes, 156 rue de Vaugirard, 75730, Paris, France

    Tamara Matysiak-Budnik

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  1. David Laharie
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  2. Sandrine Ménard
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Correspondence to Tamara Matysiak-Budnik.

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Laharie, D., Ménard, S., Asencio, C. et al. Effect of Rebamipide on the Colonic Barrier in Interleukin-10-Deficient Mice. Dig Dis Sci 52, 84–92 (2007). https://doi.org/10.1007/s10620-006-9183-4

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  • DOI: https://doi.org/10.1007/s10620-006-9183-4

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